Aquaculture International

, Volume 12, Issue 4, pp 457–466

Acute Toxicity and Anesthetic Effects of Clove Oil in Penaeus semisulcatus under Various Water Quality Conditions

  • M. Soltani
  • GH. Marmari
  • M.R. Mehrabi
Article

DOI: 10.1023/B:AQUI.0000042137.26705.7b

Cite this article as:
Soltani, M., Marmari, G. & Mehrabi, M. Aquaculture International (2004) 12: 457. doi:10.1023/B:AQUI.0000042137.26705.7b

Abstract

Acute toxicity and anesthetic effects of clove oil were studied in P. semisulcatus (1.8–2.1 g body weight). The EC50 1-h (the concentration effective for 50% of test animals), LC50 1-h (the concentration lethal to 50% of test animals after 1 h) and LC50 24-h (the concentration lethal to 50% of test animals after 24 h) were calculated at concentrations of 25, 130 and 30 mg/l, respectively, at 30°C, salinity 40 ppt, pH 8.6 and dissolved oxygen >6 mg/l. Generally, with increasing concentrations of clove oil, the times required for sedation and anesthesia decreased, while the recovery times increased. At concentrations 50, 100, 150 and 200 mg/l under temperature of 30°C and salinity of 40 ppt, the times required for sedation were 6 ± 0.2, 2.5 ± 0.3, 2 ± 0.08 and 0.5 ± 0.08 min, while times required for complete recovery were calculated to be 4.5 ± 0.3, 5.5 ± 0.17, 6.5 ± 0.25 and 11 ± 0.38 min, respectively. Also, the times required for deep anesthesia were 20 ± 1, 5 ± 0.5, 3 ± 0.4 and 2.2 ± 0.5 min in the above concentrations, while the times required for complete recovery were 10 ± 1, 11 ± 1.5, 14 ± 2.2 and 16 ± 3 min, respectively. Furthermore, considering the times to sedation, deep anesthesia and recovery at different temperatures of 20°C, 25°C, 30°C and 35°C and salinities of 25, 30, 35, 40 and 48 ppt; the combinations of salinity plus temperature and clove oil concentration plus salinity had the greatest and the least effects.

Acute toxicity Anesthesia Clove oil Penaeus semisulcatus Salinity Sedation Temperature 

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • M. Soltani
    • 1
  • GH. Marmari
    • 1
  • M.R. Mehrabi
    • 2
  1. 1.Department of Aquatic Animal Health, Faculty of Veterinary MedicineUniversity of TehranTehranIran (e-mail
  2. 2.Fisheries Research InstituteTehranIran

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