GRP94 reduces cell death in SH-SY5Y cells perturbated calcium homeostasis
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The endoplasmic reticulum (ER) resident—94 kDa glucose-regulated protein (GRP94), plays a pivotal role in cell death due to ER stress. In our study expression of GRP94 was increased in human neuroblastoma SH-SY5Y cells due to exposure to calcium ionophore A23187. A23187-mediated cell death was associated with activation of the major cysteine proteases, caspase-3 and calpain. Pretreatment with adenovirus-mediated antisense GRP94 (AdGRP94AS) reduced viability of SH-SY5Y cells subjected to A23187 treatment compared with wild type cells or cells with adenovirus-mediated overexpression of GRP94 (AdGRP94S). These results indicated that suppression of GRP94 is associated with accelerated cell death. Moreover, expression of GRP94 suppressed A23187-induced cell death and stabilized calcium homeostasis.
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- 2.Yu ZF, Luo H, Fu W, Mattson MP. The endoplasmic reticulum stress-responsive protein GRP78 protects neurons against excitotoxicity and apoptosis: Suppression of oxidative stress and stabilization of calcium homeostasis. Exp Neurology 1999; 155: 302–314.Google Scholar
- 15.Lee AS. The glucose-regulated proteins: Stress induction and clinical applications. TRENDS in Biochem Sci 2001; 26: 504–510.Google Scholar
- 18.Gregory JZ, Jin ML, Dennis WC. Reducing calcium overload in the ischemic brain. The New Eng J Med 1999; 341, 20: 1543–1544.Google Scholar
- 28.Altznauer F, Simon HU. Calpain is a major regulator of neurotrophils apoptosis. Scientific World Journal 2001; 1(S3): 91.Google Scholar