, Volume 9, Issue 4, pp 501–508 | Cite as

GRP94 reduces cell death in SH-SY5Y cells perturbated calcium homeostasis

  • Y. Bando
  • T. Katayama
  • A. N. Aleshin
  • T. Manabe
  • M. Tohyama


The endoplasmic reticulum (ER) resident—94 kDa glucose-regulated protein (GRP94), plays a pivotal role in cell death due to ER stress. In our study expression of GRP94 was increased in human neuroblastoma SH-SY5Y cells due to exposure to calcium ionophore A23187. A23187-mediated cell death was associated with activation of the major cysteine proteases, caspase-3 and calpain. Pretreatment with adenovirus-mediated antisense GRP94 (AdGRP94AS) reduced viability of SH-SY5Y cells subjected to A23187 treatment compared with wild type cells or cells with adenovirus-mediated overexpression of GRP94 (AdGRP94S). These results indicated that suppression of GRP94 is associated with accelerated cell death. Moreover, expression of GRP94 suppressed A23187-induced cell death and stabilized calcium homeostasis.

calcium homeostasis endoplasmic reticulum molecular chaperone neuronal cell death 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Y. Bando
    • 1
  • T. Katayama
    • 1
    • 2
  • A. N. Aleshin
    • 3
  • T. Manabe
    • 1
  • M. Tohyama
    • 1
    • 2
  1. 1.Department of Anatomy and NeuroscienceOsaka University Graduate School of MedicineOsaka
  2. 2.Core Research for Evolutional Science and TechnologyJapan Science and Technology (CREST JST)Saitama
  3. 3.Division of Cardiovascular Surgery, Department of SurgeryOsaka University Graduate School of MedicineOsakaJapan

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