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Antonie van Leeuwenhoek

, Volume 85, Issue 3, pp 229–235 | Cite as

Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum

  • Leonardo Velasco
  • Socorro Mesa
  • Chang-ai Xu
  • María J. Delgado
  • Eulogio J. Bedmar
Article

Abstract

The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a PnosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK2.

Bradyrhizobium japonicum Denitrification Microerobiosis Respiratory nitrous oxide reductase 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Leonardo Velasco
    • 1
  • Socorro Mesa
    • 2
  • Chang-ai Xu
    • 3
  • María J. Delgado
    • 4
  • Eulogio J. Bedmar
    • 4
  1. 1.Departamento de Biotecnología. Centro de Investigación y Desarrollo AgroalimentarioMurciaSpain
  2. 2.Institut für MikrobiologieZürichSwitzerland
  3. 3.Agriculture and Agri-Food Canada, Potato Research CentreFredericton, NBCanada
  4. 4.Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSICGranadaSpain

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