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Russian Journal of Genetics

, Volume 37, Issue 12, pp 1343–1347 | Cite as

High Frequency Conjugative Mobilization Accomplished by a Natural Bacillus subtilisStrain Carrying a Large Plasmid

  • O. V. Lotareva
  • V. Z. Nezametdinova
  • E. A. Fedorina
  • E. U. Poluektova
  • M. A. Titok
  • A. A. Prozorov
Article

Abstract

Conjugative properties of the strain Bacillus subtiliscarrying a large plasmid approximately 95 kb in size and isolated in Belarus from forest soil were described. The staphylococcal plasmid pUB110 that had previously been introduced into this strain was transferred to recipient cells of the Bacillus subtilis168 strain with a frequency of approximately 10–2. The transfer occurred with approximately the same frequency both upon donor and recipient cell contact on the surface of membranes and in a liquid medium. The latter fact makes this system suitable as a model for studying conjugative mobilization in bacilli. A large plasmid cannot be transferred to recipients. An optimal temperature for conjugation of donor and recipient cells was 37°C, but conjugation also proceeded at lower temperatures, up to 21°C.

Keywords

Liquid Medium Forest Soil Bacillus Subtilis168 Cell Contact Recipient Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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REFERENCES

  1. 1.
    Koretskaya, N.G., Svetoch, O.E., and Dobritsa, A.P., Conjugative Transfer of Plasmids between Bacillus spp., Dokl. Akad. Nauk SSSR, 1988, vol. 303, no. 2, pp. 488-491.Google Scholar
  2. 2.
    Reddy, A., Battisti, L., and Thorne, C.B., Identification of Self-Transmissible Plasmids in Four Bacillus thuringiensis Subspecies, J. Bacteriol., 1987, vol. 169, no. 11, pp. 52 263-52 270.Google Scholar
  3. 3.
    Andrup, L., Damgaard, J., and Wassermann, K., Mobilization of Small Plasmids in Bacillus thuringiensis subsp. israelensis Is Accompanied by Specific Aggregation, J. Bacteriol., 1993, vol. 175, no. 20, pp. 6530-6536.Google Scholar
  4. 4.
    Andrup, L., Bendixen, Y., and Jensen, G., Mobilization of Bacillus thuringiensis Plasmid PTX14-3, Plasmid, 1995, vol. 33, no. 3, pp. 159-167.Google Scholar
  5. 5.
    Andrup, L., Jorgensen, O., Wilcks, A., et al., Mobilization of “Nonmobilizable” Plasmids by the Aggregation-Mediated Conjugation System of Bacillus thuringiensis, Plasmid, 1996, vol. 36, no. 1, pp. 75-85.Google Scholar
  6. 6.
    Poluektova, E.U., Nezametdinova, V.Z., and Prozorov, A.A., A Large Conjugative Plasmid from a Soil Bacillus subtilis Strain, Genetika (Moscow), 2000, vol. 36, no. 7, pp. 1000-1002.Google Scholar
  7. 7.
    Kohler, T. and Thorne, C., Bacillus subtilis (natto) Plasmid PLS20 Mediates Intraspecies Plasmid Transfer, J. Bacteriol., 1987, vol. 169, no. 11, pp. 5271-5278.Google Scholar
  8. 8.
    Chang, S. and Cohen, S., High Frequency Transformation of Bacillus subtilis Protoplasts by Plasmid DNA, Mol. Gen. Genet., 1979, vol. 168, no. 1, pp. 111-115.Google Scholar
  9. 9.
    Nezametdinova, V.Z., Poluektova, E.U., and Prozorov, A.A., Construction of Plasmids Integrating in the Bacillus subtilis Chromosome through Homologous Recombination and Their Use as Integrative Vectors, Genetika (Moscow), 1987, vol. 23, no. 3, pp. 405-414.Google Scholar
  10. 10.
    Birnboim, H. and Doly, J., A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA, Nucleic Acids Res., 1979, vol. 7, no. 6, pp. 1513-1523.Google Scholar
  11. 11.
    Lotareva, O.V., Poluektova, E.U., Titok, M.A., and Prozorov, A.A., A Large Plasmid from a Soil Bacillus subtilis Strain Determines a High Frequency of Conjugative Mobilization, Dokl. Akad. Nauk (in press).Google Scholar
  12. 12.
    Clark, A. and Warren, G., Conjugative Transmission of Plasmids, Annu. Rev. Genet., 1979, vol. 19, pp. 99-125.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • O. V. Lotareva
    • 1
  • V. Z. Nezametdinova
    • 1
  • E. A. Fedorina
    • 1
  • E. U. Poluektova
    • 1
  • M. A. Titok
    • 2
  • A. A. Prozorov
    • 1
  1. 1.Vavilov Institute of General Genetics, Russian Academy of SciencesMoscowRussia
  2. 2.Department of MicrobiologyBelarussian State UniversityMinskBelarus

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