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Genetic Resources and Crop Evolution

, Volume 47, Issue 3, pp 323–334 | Cite as

Genetic diversity in populations of wild diploid wheat Triticum urartu Tum. ex. Gandil. revealed by isozyme markers

  • Mohammad Moghaddam
  • Bahman Ehdaie
  • J. Giles Waines
Article

Abstract

Genetic variation and its distribution within and among 23 populations of Triticum urartu collected from Syria, Lebanon, Turkey, Armenia, and Iran was estimated using isozyme markers at eight polymorphic loci. The number of alleles per locus (A= 1.21), percentage polymorphic loci (P= 20.1%), and mean gene diversity (He= 0.024) were relatively low. In a population from Lebanon, a high number of alleles per locus (A= 2.13) and percentage polymorphic loci (P= 87.5%) was found. On average, genetic variation among populations (GST= 0.407) was smaller than within-population variation (0.593). However, different patterns of genetic structure were found among various geographic regions. Interpopulation variation was highest for the Iranian populations (0.89) followed by the Turkish populations (0.66). A reverse pattern was observed for the Syrian (0.11) and for the Lebanese (0.13) populations. The Armenian populations exhibited similar interpopulation and within-population variation. Principal component and cluster analyses resulted in distinct grouping of the geographically proximal populations, with the exception of the two Iranian populations. The Turkish populations were different from the neighboring Armenian populations compared to other countries. The populations from southern Syria and those from Lebanon also exhibited a high degree of genetic diversity. The two most heterozygous loci, Mdh-2 and Pgi-2, separated the populations along the first and second principal components, respectively. Most of the rare alleles were scattered sporadically throughout the geographic regions. Rare alleles with high frequencies were found in the Turkish and Armenian populations. These results indicated that different geographic regions require specific sampling procedures in order to capture the range of genetic variation observed in T. urartu populations.

biodiversity germplasm collection isozyme markers Triticum urartu Tum. ex. Gandil 

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References

  1. Brown, A.D.H., 1978. Isozymes, plant population genetic structure and genetic conservation. Theor. Appl. Genet. 52: 145–157.Google Scholar
  2. Brown, A.H.D. & J.J. Burdon, 1987. Mating systems and colonizing success in plants. In: Gray, A.J., M.J. Crawley & P.J. Edwards (Eds.), Colonization, Succession and Stability, pp. 115–131. Blackwell Scientific Publication, U.K.Google Scholar
  3. Castagna, R., S. Gnocchi, M. Perenzin & M. Heun, 1997. Genetic variability of the wild diploid wheat Triticum urartu revealed by RFLP and RAPD markers. Theor. Appl. Genet. 94: 424–430.Google Scholar
  4. Chapman, V., T.E. Miller & R. Riley, 1976. Equivalence of the A genome of bread wheat and that of T. urartu. Genet. Res. 27: 69–76.Google Scholar
  5. Cox, T.S., 1991. The contribution of introduced germplasm to the development of U.S. wheat cultivars. In: Shands, H.L. & L.E. Wiesner (Eds.), Use of Plant Introductions in Cultivar Development, pp. 25-47, Part 1, CSSA Special Publication No. 17, Madison, WI, USA.Google Scholar
  6. Dhaliwal, H.S. & B.L. Johnson, 1976. Anther morphology and the origin of the tetraploid wheats. Am. J. Bot. 63: 363–368.Google Scholar
  7. Dhaliwal, H.S., J.S. Sidhu & J.L. Minocha, 1993. Genetic diversity in diploid and hexaploid wheats as revealed by RAPD markers. Crop. Improv. 20: 17–20.Google Scholar
  8. Dudnikov, A.J.U., 1998. Allozyme variation in Transcaucasian populations of Aegilops squarrosa. Heredity 80: 248–258.Google Scholar
  9. D'Ovidio, R., O.A. Tanzarella, A. Cenci, E. Iacono & E. Porceddu, 1994. RFLP analysis in wheat. Isolation and chromosomal assignment of digoxigenin-labelled clones. J. Genet. Breed. 48: 73–80.Google Scholar
  10. Dvorák, J., 1976. The relationship between the genome of Triticum urartu and the A and B genomes of Triticum aestivum. Can. J. Genet. Cytol. 18: 371–377.Google Scholar
  11. Dvorák, J., P.E. McGuire & B. Cassidy, 1988. Apparent sources of the A genomes of wheats inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. Genome 30: 680–689.Google Scholar
  12. Dvorák, J., P. Di Terlizzi, H.B. Zhang & P. Resta, 1993. The evolution of polyploid wheats: identification of the A genome donor species. Genome 36: 21–31.Google Scholar
  13. Ellstrand, N.C., 1984. Multiple paternity within the fruits of the wild radish, Raphanus sativus. Am. Nat. 123: 819–828.Google Scholar
  14. Firat, A.E. & A. Tan, 1998. Ecogeography and distribution of wild cereals in Turkey. In: Zencirci, N., K. Kaya, Y. Anikster & W.T. Adams (Eds.), Proc. Int. Symp. In Situ Conservation of Plant Genetic Diversity, pp. 81–85. Central Res. Inst. For Field Crops, Ankara, Turkey.Google Scholar
  15. Gale, M.D. & P.J. Sharp, 1988. Genetic markers in wheat-developments and prospects, pp. 469-475. Proc. 7th. Int. Wheat Genet. Symp., Cambridge, U.K.Google Scholar
  16. Garvin, D.F., M.L. Roose & J.G. Waines, 1989. Isozyme genetics and linkage in tepary bean, Phaseolus acutifolis A. Gray. J. Hered. 80: 373–376.Google Scholar
  17. Hamrick, J.L., Y.B. Linhart & J.B. Mitton, 1979. Relationships between life history characteristics and electrophoreticallydetectable genetic variation in plants. Annu. Rev. Ecol. Syst. 10: 173–200.Google Scholar
  18. Harlan, J.R, 1976. Genetic resources in wild relatives of crops. Crop Sci. 16: 329–333.Google Scholar
  19. Jaaska, V., 1993. Isoenzymes in the evaluation of germplasm diversity in wild diploid relatives of cultivated wheat. In: Damania, A.B. (Ed.), Biodiversity and Wheat Improvement, pp. 247–257. John Wiley & Sons, New York, NY.Google Scholar
  20. Jakubizner, M.M., 1959. New wheat species. In: Jenkins, BC (Ed.), pp. 207-217. Proc. 1st Int. Wheat Genet. Symp., Winnipeg, Canada.Google Scholar
  21. Johnson, B.L., 1975. Identification of the apparent B-genome donor of wheat. Can. J. Genet. Cytol. 17: 21–39.Google Scholar
  22. Johnson, B.L. & H.S. Dhaliwal, 1978. Triticum urartu and genome evolution in tetraploid wheats. Am. J. Bot. 65: 907–918.Google Scholar
  23. Kimber, G. & E.R. Sears, 1987. Evolution in the genus Triticum and the origin of cultivated wheat. In: Heyne, E.G. (Ed.), Wheat and Wheat Improvement, pp. 154–163. American Society of Agronomy, Madison, WI, USA.Google Scholar
  24. Le Corre, V. & M. Bernard, 1995. Assessment of the type and degree of restriction fragment length polymorphism (RFLP) in diploid species of the genus Triticum. Theor. Appl. Genet. 90: 1063–1067.Google Scholar
  25. Lewis, P.O., 1992. GeneStat-PC 3.3. Department of Statistics, Raleigh, North Carolina University, NC, USA.Google Scholar
  26. Marshall, D.R. & R.W. Allard, 1970. Maintenance of isozyme polymorphism in natural populations of Avena barbata. Genetics 66: 393–399.Google Scholar
  27. Nei, M., 1973. Analysis of gene diversity in sub-divided populations. Proc. Natl. Acad. Sci. USA 70: 3321–3323.Google Scholar
  28. Nei, M., 1978. Estimation of average heterozygosity and genetic distance from small number of individuals. Genetics 89: 583–590.Google Scholar
  29. Poulik, M.D., 1957. Starch gel electrophoresis in discontinuous system of buffers. Nature 180: 1477–1479.Google Scholar
  30. Rafi, M.M., B. Ehdaie & J.G. Waines, 1992. Quality traits, carbon isotope discrimination and yield components in wild wheats. Ann. Bot. 69: 467–474.Google Scholar
  31. Rohlf, F.J., 1993. Numerical taxonomy and multivariate analysis system, version 1.80. 100 North Country Road, Setauket, NY.Google Scholar
  32. Skovmand, B. & S. Rajaram, 1990. Utilization of genetic resources in the improvement of hexaploid wheat. In: Srivastava, J.P. & A.B. Damania (Eds.), Wheat genetic resources: meeting diverse needs, pp. 259–268. John Wiley & Sons, New York, NY.Google Scholar
  33. Slatkin, M. & N.H. Barton, 1989. A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43: 1349–1368.Google Scholar
  34. Smith-Huerta, N.L., A.J. Huerta, D. Barnhart & J.G. Waines, 1989. Genetic diversity in wild diploid wheats Triticum monococcum var. boeoticum and T. urartu (Poaceae). Theor. Appl. Genet. 78: 260–264.Google Scholar
  35. Soltis, D.E., C.H. Haufler, D.C. Darrow & G.J. Gastony, 1983. Starch gel electrophoresis of fern: a compilation of grinding buffers, gel and electrode buffers, and staining schedules. Am. Fern. J. 73: 9–27.Google Scholar
  36. Takumi, S., S. Nasuda, Y.G. Liu & K. Tsunewaki, 1993. Wheat phylogeny determined by RFLP analysis of nuclear DNA. 1. Einkorn wheat. Jpn. J. Genet. 68: 73–79.Google Scholar
  37. Vierling, R.A. & H.T. Nguyen, 1992. Use of RAPD markers to determine the genetic diversity of diploid wheat genotypes. Theor. Appl. Genet. 84: 835–838.Google Scholar
  38. Waines, J.G. & P.I. Payne, 1987. Electrophoretic analysis of the high-molecular-weight glutenin subunits of Triticum monococcum, T. urartu and the A genome of bread wheat (T. aestivum). Theor. Appl. Genet. 74: 71–76.Google Scholar
  39. Waines, J.G., B. Ehdaie & D. Barnhart, 1987. Variability in Triticum and Aegilops species for seed characteristics. Genome 29: 41–46.Google Scholar
  40. Wendel, J.F. & N.F. Weeden, 1989. Visualization and interpretation of plant isozymes. In: Soltis, D.E. & P.S. Soltis (Eds.), Isozymes in Plant Biology, pp. 5–45. Dioscorides Press, OR, USA.Google Scholar
  41. Yaghoobi-Saray, J., 1979. An electrophoretic analysis of genetic variation within and between populations of five species in Triticum-Aegilops complex. Ph.D. Thesis, University of California, Davis, CA, USA.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Mohammad Moghaddam
    • 1
  • Bahman Ehdaie
    • 2
  • J. Giles Waines
    • 3
  1. 1.Department of Agronomy, College of AgricultureTabriz UniversityTabrizIran
  2. 2.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideU.S.A.
  3. 3.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideU.S.A.

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