Glucotropaeolin and myrosinase production in hairy root cultures of Tropaeolum majus
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We obtained callus, cell suspension and hairy root cultures of Tropaeolum majus and we demonstrated their ability to produce glucotropaeolin and myrosinase. In hairy roots glucotropaeolin content and myrosinase activity were higher in comparison with callus, cell suspension and leaves of intact plants. In hairy root cultures the highest glucotropaeolin contents were detected on the 9th day of culture. In relation to dry weight glucotropaeolin production by hairy roots was stimulated markedly by cystein and less by phenylalanine and methyl jasmonate. Cystein inhibited biomass growth so in relation to culture volume stimulating effect of cystein on glucotropaeolin yield declined. Myrosinase activity was stimulated by methyl jasmonate. The ratio of myrosinase activity to glucotropaeolin content in cultures with methyl jasmonate was much higher than in cultures with cystein.
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- Bones A & Slupphaug G (1989) Purification, characterization and partial amino acid sequencing of β-thioglucosidase from Brassica napus L. J. Plant Physiol. 134: 722–729Google Scholar
- Danielak R (1973) Glukozynolaty i siarczki czosnku. In: Borkowski B (Ed.) Chromatografia Cienkowarstwowa w Analizie Farmaceutycznej (pp 123–125). PZWL, WarszawaGoogle Scholar
- Eilert U (1987) Elicitation methodology and aspects of application. In: Vasil IR & Constabel F (eds) Cell Culture and Somatic Cell Genetics of Plants, Vol. 4(pp 153–196). Academic Press, OrlandoGoogle Scholar
- Kirkland DF, Matsuo M & Underhill EW (1971) Detection of glucosinolates and myrosinase in plant tissue cultures. Cited from: Iqbal MCM, Röbbelen G & Möllers C (1995) Biosynthesis of glucosinolates by microspore derived embryoids and plantlets in vitro of Brassica napus L. Plant Sci. 112: 107–115Google Scholar
- Mayton HS, Olivier C, Vaughn SF & Loria R (1996) Correlation of fungicidal activity of Brassica species with allyl isothiocyanate production in macerated leaf tissue. Phytopathology 86: 267–271Google Scholar