Plant Cell, Tissue and Organ Culture

, Volume 49, Issue 1, pp 39–44 | Cite as

Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’

  • Geert-Jan De Klerk
  • Jolanda Ter Brugge
  • Svetla Marinova


We have examined in vitro rooting of apple ‘Jork 9‘ shoots exposed for three weeks to each of the three auxins commonly used for ex vitro rooting: indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA). During the initial five days of the rooting treatment, the cultures were incubated in darkness. In this period, the root initials are formed. Then, the cultures were moved to the light. NAA resulted in a low (ca. 8 roots), and IAA or IBA in a high (ca. 15 roots) maximal root number. The maximal root number was reached at a wide range of IAA concentrations (10-100 μM) but at only one concentration of IBA (10 μM) or NAA (3 μM). With NAA and IBA, growth of roots and shoots was much more inhibited than with IAA. For these reasons, IAA is the preferable auxin for in vitro rooting of apple ‘Jork 9’ shoots.

apple auxin micropropagation rooting woody plants 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Geert-Jan De Klerk
    • 1
  • Jolanda Ter Brugge
    • 1
  • Svetla Marinova
    • 1
  1. 1.Centre for Plant Tissue Culture ResearchLisseThe Netherlands

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