Enzyme Aided Analysis of the Substituent Distribution along the Chain of Cellulose Acetates Regioselectively Modified by the Action of an Aspergillus niger Acetylesterase
Two cellulose acetates (CA) were regioselectively deacetylated by action of a pure Aspergillus niger acetylesterase from the carbohydrate esterase family 1. The action of acetyl esterase along the polymeric chain was monitored by a new enzyme-aided method. CA with and without esterase modification was hydrolysed with a pure endoglucanase. The fragments were deutero-acetylated and separated by preparative SEC in CHCl3. The partial degree of substitution of the individual fragments was determined by 1H-NMR spectroscopy. The investigation confirmed the uniform regioselective and regular deacetylation along the polysaccharide chain. The partial substitution in C2 seemed to be of major importance for the enzyme's mode of action.
Unable to display preview. Download preview PDF.
- Altaner C., Saake B. and Puls J. 2001. Mode of action of acetyles-terases associated with endoglucanases towards water-soluble and-insoluble cellulose acetates. Cellulose 8: 259–265.Google Scholar
- Altaner C., Saake B. and Puls J. 2003a. Specificity of anAspergillus niger esterase deacetylating cellulose acetate. Cellulose 10: 85–95.Google Scholar
- Altaner C., Saake B., Tenkanen M., Eyzaguine J., Faulds C. B., Biely P., Siika-aho M. and Puls J. 2003b. Regioselective deace-tylation of cellulose acetates by acetyl xylan esterases. J. Biotecbnol. (in press).Google Scholar
- Deus C., Friebolin H. and Siefert E. 1991. Paitiell acetylierte Cellulose-Synthese mid Bestimmung der Substituentenverteilung mit Hike der 111 NMR-Spektroskopie. Makromol. Chem. 192: 75–83.Google Scholar
- Goodlett V. M., Doughert I. T. and Patton H.W. 1971. Characterization of cellulose acetates by nuclear magnetic resonance. J. Polym. Sci. Part A-i 9: 155–161.Google Scholar
- Iijima H., Kowsaka K. and Kamide K. 1992. Detemñnation of sequence distribution of substituted and unsubstituted glucopyranose uthts in water-soluble cellulose acetate chain as revealed by enzymic degradation. Polym. J. (Tokyo) 24: 1077–1097.Google Scholar
- Lee S.-J., Altaner C., Puls J. and Saake B. (2003) Deteimination of the substituent distribution along cellulose acetate chains as revealed by enzymatic and chemical methods. Carbohydr. Polym. (in press).Google Scholar
- Puls J. and Dokk Glawiscimig T. 1997. A new approach for the preparation of lowest molecular weight cellulose. In: Leary G. J. and Gray D. G. Proceedings of the 9 International Symposium on Wood and Pulping Chemistry-Oral Presentatiois. ISV/PC, Montreal, C2–1–C2–4.Google Scholar