Plant Growth Regulation

, Volume 41, Issue 1, pp 13–24 | Cite as

Cytokinins in macroalgae

  • W.A. Stirk
  • O. Novák
  • M. Strnad
  • J. van Staden


Thirty-one seaweeds were collected from the warmer KwaZulu-Natal coast and the cooler Cape waters (South Africa). Plant material was extracted with 70% ethanol supplemented with deuterium labelled standards of all known isoprenoid cytokinins. The samples were then centrifuged and purified by combined DEAE-Sephadex×octadecylsilica column and immunoaffinity chromatography and finally analysed for cytokinins by HPLC-linked mass spectrometry and a photodiode array detector. The cytokinin profiles were similar in all the macroalgae regardless of their taxonomy and growing locality. The main type of isoprenoid cytokinins present were zeatins with cis forms being more common than trans forms and isopentenyladenine (iP) derivatives. Only a few dihydrozeatin-type cytokinins were detected at very low levels in only nine species. Aromatic cytokinins were also present but at lower levels and were represented by benzyladenine (BA) and ortho- and meta-topolin derivatives. The topolins were present in greater diversity and concentrations than BA. For all the cytokinin types, the free bases, O-glucosides and nucleotides were the most common with no N-glucosides being detected and ribosides present at very low levels. The results suggest that different pathways for regulating cytokinin concentrations operate in macroalgae than in higher plants.

Aromatic cytokinins Cytokinin content Isoprenoid cytokinins Macroalgae 


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  1. Auer C.A.1997. Cytokinin conjugation: recent advances and patterns in plant evolution. Plant Growth Regul.23: 17–32.Google Scholar
  2. åstot C., Dolezal K., Nordström A., Wang Q., Kunkel T., Moritz T., Chau N. and Sandberg G.2000. An alternative cytokinin biosynthesis pathway. Proc. Natl. Acad. Sci. USA97: 14778–14783.Google Scholar
  3. Binns A.N.1994. Cytokinin accumulation and action: Biochemical, genetic, and molecular approaches. Annu. Rev. Plant Physiol. Plant Mol. Biol.45: 173–196.Google Scholar
  4. Burkiewicz K.1987. The influence of gibberellins and cytokinins on the growth of some unicellular Baltic algae. Bot. Mar.30: 63–69.Google Scholar
  5. Chen C.M.1997. Cytokinin biosynthesis and interconversion. Physiol. Plant.101: 665–673.Google Scholar
  6. de Nys R., Jameson P.E., Chin N., Brown M.T. and Sanderson K.J.1990. The cytokinins as endogenous growth regulators in Macrocystis pyrifera (L.) C.Ag. (Phaeophyceae). Bot. Mar.33: 467–475.Google Scholar
  7. de Nys R., Jameson P.E. and Brown M.T.1991. The influence of cytokinins on the growth of Macrocystis pyrifera. Bot. Mar.34: 465–467.Google Scholar
  8. Duan D., Lui X., Pan F., Lui H., Chen N. and Fei X.1995. Extraction and identification of cytokinin from Laminaria japonica Aresch. Bot. Mar.38: 409–412.Google Scholar
  9. Faiss M., Zalubilová J., Strnad M. and Schmulling T.1997. Conditional expression of the ipt gene indicates a function for cytokinins in paracrine signalling in whole tobacco plants. Plant J.12: 401–415.Google Scholar
  10. Featonby-Smith B.C. and van Staden J.1984. Identification and seasonal variation of endogenous cytokinins in Ecklonia maxima (Osbeck) Papenf. Bot. Mar.27: 527–531.Google Scholar
  11. Holub J., Hanuš J., Hanke D.E. and Strnad M.1998. Biological activity of cytokinins derived from ortho-and meta-hydroxybenzyladenine. Plant Growth Regul.26: 109–115.Google Scholar
  12. Jennings R.C.1969. Cytokinins as endogenous growth regulators in the algae Ecklonia (Phaeophyta) and Hypnea (Rhodophyta). Aus. J. Biol. Sci.22: 621–627.Google Scholar
  13. Jennings R.C., Broughton W.J. and McComb A.J.1972. Effect of kinetin on the phycoerythrin and chlorophyll content of a red algae. Phytochemistry11: 1937–1943.Google Scholar
  14. Kakimoto T.2001. Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases. Plant Cell Physiol.42: 677–685.Google Scholar
  15. Letham D.S. and Palni L.M.S.1983. The biosynthesis and metabolism of cytokinins. Annu. Rev. Plant Physiol.34: 163–197.Google Scholar
  16. MacDonald E.M.S., Akiyoishi D.E. and Morris R.O.1981. Combined high-performance liquid chromatography-radioimmunoassay for cytokinins. J. Chromatogr.214: 101–109.Google Scholar
  17. Martin R.C., Mok M.C., Shaw G. and Mok D.W.S.1989. An enzyme mediating the conversion of zeatin to dihydrozeatin in Phaseolus embryos. Plant Physiol.90: 1630–1635.Google Scholar
  18. Martin R.C., Mok M.C., Habben J.E. and Mok D.W.S.2001. A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-zeatin. Proc. Natl. Acad. Sci. USA98: 5922–5926.Google Scholar
  19. Mok M.C., Martin R.C. and Mok D.W.S.2000. Cytokinins: Biosynthesis, metabolism and perception. In vitro Cell. Develop. Biol.36: 102–107.Google Scholar
  20. Mok D.W.S. and Mok M.C.2001. Cytokinin metabolism and action. Annu. Rev. Plant Physiol. Plant Mol. Biol.52: 89–118.Google Scholar
  21. Mooney P.A. and van Staden J.1984. Seasonal changes in the levels of endogenous cytokinins in Sargassum heterophyllum (Phaeophyceae). Bot. Mar.27: 437–442.Google Scholar
  22. Novák O., Tarkowski P., Tarkowská D., Dolezal K., Lenobel R. and Strnad M.2003. Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry. Anal. Chim. Acta480: 207–218.Google Scholar
  23. Pedersén M.1973. Identification of a cytokinin, 6-(3-methyl-2-butenylamino)purine, in seawater and the effect of cytokinins on brown algae. Physiol. Plant.28: 101–105.Google Scholar
  24. Rhijn J.A., Heskamp H.H., Davelaar E., Jordi W., Leloux M.S. and Brinkman U.A.T.2001. Quantitative determination of glucosylated and aglycon isoprenoid cytokinins at subpicomolar levels by microcolumn liquid chromatography combined with electrospray tandem mass spectrometry. J. Chromatogr.A929: 31–42.Google Scholar
  25. Sakakibara H. and Takei K.2002. Identification of cytokinin biosynthesis genes in Arabidopsis: A breakthrough for understanding the metabolic pathway and the regulation in higher plants. J. Plant Growth Regul.21: 17–23.Google Scholar
  26. Schwender J., Gemünden C. and Lichtenthaler H.K.2001. Chlorophyta exclusively use the 1-deoxyxylulose 5-phosphate/2-C-methylerythritol 4-phosphate for the biosynthesis of isoprenoids. Planta212: 416–423.Google Scholar
  27. Stirk W.A. and van Staden J.1997. Isolation and identification of cytokinins in a new commercial seaweed product made from Fucus serratus L. J. Appl. Phycol.9: 327–330.Google Scholar
  28. Strnad M.1997. The aromatic cytokinins. Physiol. Plant.101: 674–688.Google Scholar
  29. Takei K., Sakakibara H. and Sugiyama T.2001. Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana. J. Biol. Chem.276: 26405–26410.Google Scholar
  30. Tay S.A.B., MacLeod J.K., Palni L.M.S. and Letham D.S.1985. Detection of cytokinins in a seaweed extract. Phytochemistry23: 2611–2614.Google Scholar
  31. Tay S.A.B., Palni L.M.S. and MacLeod J.K.1987. Identification of cytokinin glucosides in a seaweed extract. Plant Growth Regul.5: 133–138.Google Scholar
  32. van Staden J. and Drewes F.E.1991. The biological activity of cytokinin derivatives in the soybean callus bioassay. Plant Growth Regul.10: 109–115.Google Scholar
  33. Zazímalová E., Kamínek M., Brezinová A. and Motyka V.1999. Control of cytokinin biosynthesis and metabolism. In: Hooykaas P.J.J., Hall M.A. and Libbenga K.R. (eds), Biochemistry and Molecular Biology of Plant Hormones. Elsevier Sciences, Amsterdam, The Netherlands, pp. 141–160.Google Scholar
  34. Zhang W., Yamane H., Takahashi N., Chapman D.J. and Phinney B.O.1989. Identification of a cytokinin in the green alga Chara globularis. Phytochemistry28: 337–338.Google Scholar
  35. Zhang W., Chapman D.J., Phinney B.R. and Spray C.R.1991. Identification of cytokinins in Sargassum muticum (Phaeophyta) and Porphyra perforata (Rhodophyta). J. Phycol.27: 87–91.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • W.A. Stirk
    • 1
  • O. Novák
    • 1
  • M. Strnad
    • 1
  • J. van Staden
    • 1
  1. 1.Research Centre for Plant Growth and DevelopmentUniversity of Natal PietermaritzburgScottsvilleSouth Africa

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