Biodegradation

, Volume 14, Issue 6, pp 367–372 | Cite as

Degradation of polycyclic aromatic hydrocarbons and long chain alkanes at 6070 °C by Thermus and Bacillus spp

  • Heiko Feitkenhauer
  • Rudolf Müller
  • Herbert MAuml;rkl

Abstract

Although polycyclic aromatic hydrocarbons (PAH) and alkanesare biodegradable at ambient temperature, in some cases low bioavailabilities are thereason for slow biodegradation. Considerably higher mass transfer rates and PAH solubilities and hence bioavailabilities can be obtained at higher temperatures. Mixed and pure cultures of aerobic, extreme thermophilic microorganisms (Bacillus spp., Thermus sp.) were used to degrade PAH compounds and PAH/alkane mixtures at 65 °C. The microorganismsused grew on hydrocarbons as sole carbon and energy source. Optimal growthtemperatures were in the range of 60–70 °C at pH values of 6–7. The conversion of PAH with 3–5 rings (acenaphthene, fluoranthene, pyrene, benzo[e]pyrene) was demonstrated. Efficient PAH biodegradation required a second, degradable liquid phase. Thermus brockii Hamburg metabolized up to 40 mg (l h)-1 pyrene and 1000 mg(1 h)-1 hexadecane at 70 °C. Specific growth rates of 0.43 h-1 were measured for this strain with hexadecane/pyrene mixtures as the sole carbon and energy source in a 2-liter stirred bioreactor. About 0.7 g cell dry weight were formed from 1 g hydrocarbon. The experiments demonstrate the feasibility and efficiency of extreme thermophilic PAH and alkane biodegradation.

aerobe bioavailability biodegradation polycyclic aromatic hydrocarbons alkanes extreme thermophilic microorganisms 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Heiko Feitkenhauer
    • 1
  • Rudolf Müller
    • 2
  • Herbert MAuml;rkl
    • 3
  1. 1.Laboratory of Chemical Engineering and Industrial ChemistryETH Zürich, HönggerbergZürichSwitzerland
  2. 2.Institute of Technical BiochemistryTechnical University of Hamburg-HarburgHamburgGermany
  3. 3.Institute of Bioprocess and Biochemical EngineeringTechnical University of Hamburg-HarburgHamburgGermany

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