Journal of Analytical Chemistry

, Volume 58, Issue 11, pp 1038–1042 | Cite as

Determination of the Dissociation Constant and Stoichiometry of a Complex of the Protein Interferon α-2b with Cibacron Blue F3G-A

  • Zh. Bumeliene
  • I. Sereikaite
  • V.-A. Bumelis
  • V. Smirnovas
  • G. Gedminiene
  • L. Braziunaite
  • E. Bajorunaite
Article

Abstract

The nonlinear least-squares technique was used for determining the complexation parameters of the recombinant human cytokine interferon α-2b with a pseudobiospecific ligand, Cibacron Blue F3G-A. The dissociation constant of the complex and the number of ligand–protein binding sites were found.

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Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • Zh. Bumeliene
    • 1
  • I. Sereikaite
    • 1
  • V.-A. Bumelis
    • 1
  • V. Smirnovas
    • 1
  • G. Gedminiene
    • 1
  • L. Braziunaite
    • 1
  • E. Bajorunaite
    • 1
  1. 1.Faculty of Fundamental SciencesGediminas Technical UniversityVilniusLithuania

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