Determination of the Dissociation Constant and Stoichiometry of a Complex of the Protein Interferon α-2b with Cibacron Blue F3G-A
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The nonlinear least-squares technique was used for determining the complexation parameters of the recombinant human cytokine interferon α-2b with a pseudobiospecific ligand, Cibacron Blue F3G-A. The dissociation constant of the complex and the number of ligand–protein binding sites were found.
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