, Volume 14, Issue 6, pp 397–406 | Cite as

Microsomal Transformation of Organophosphorus Pesticides by White Rot Fungi

  • Juan Jauregui
  • Brenda Valderrama
  • Arnulfo Albores
  • Rafael Vazquez-Duhalt


The enzymatic mechanism for the transformationof organophosphorus pesticides (OPPs) by differentwhite-rot fungi strains was studied. With theexception of Ganoderma applanatum 8168,all strains from a collection of 17 different fungicultures were able to deplete parathion. Threestrains showing the highest activities were selectedfor further studies: Bjerkandera adusta 8258,Pleurotus ostreatus 7989 and Phanerochaetechrysosporium 3641. These strains depleted 50 to96% of terbufos, azinphos-methyl, phosmet andtribufos after four-days exposure to the pesticides.In order to identify the cellular localization of thetransformation activity, the extracellular andmicrosomal fractions of Pleurotus ostreatus7989 were evaluated in vitro. While the activitiesof ligninolytic enzymes (lignin peroxidase,manganese peroxidase and laccase) weredetected in the extracellular fraction, noenzymatic modification of any of the fivepesticides tested could be found, suggestingthe intracellular origin of the transformationactivity. In accordance with this observation themicrosomal fraction was found able to transformthree OPPs with the following rates:10 μmol mg prot-1 h-1 forphosmet, 5.7 μmol mg prot-1 h-1 forterbufos, and 2.2 μmol mg prot-1 h-1 forazinphos-methyl. The products from these reactions andfrom the transformation of trichlorfon and malathion,were identified by mass-spectrometry. These results,supported by specific inhibition experiments and thestringent requirement for NADPH during the in vitroassays suggest the involvement of a cytochrome P450.

biotransformation cytochrome P450 fungi microsomal organophosphorus pesticides 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Juan Jauregui
    • 1
  • Brenda Valderrama
    • 1
  • Arnulfo Albores
    • 2
  • Rafael Vazquez-Duhalt
    • 1
  1. 1.Instituto de BiotecnologiaCuernavaca, MorelosMexico
  2. 2.CINVESTAV-IPNMéxico D.FMexico

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