Advertisement

Photosynthetica

, Volume 38, Issue 1, pp 143–148 | Cite as

Linear Dichroism, Fluorescence Polarization, and Path of the Thermal Deactivation of Excited Cyanobacterial (Synechococcus Elongatus) Photosystem 1 Immobilized and Oriented in Polymer Films

  • G.E. Białek-Bylka
  • D. Sofrová
  • J. Szurkowski
  • R. Skwarek
  • B. Sopko
  • H. Manikowski
Article

Abstract

Pigment-protein complexes enriched in photosystem 1 (PS1) and, for comparison, enriched in photosystem 2 (PS2) were isolated from the cyanobacterium Synechococcus elongatus Nag. f. thermalis Geitl. They were immobilized and oriented in the polyvinyl alcohol (PVA) films, and studied by linear dichroism (LD), fluorescence polarization (FP), photoacoustic spectroscopy (PAS), and polarized photoacoustic spectroscopy (PAS and PAS). The LD signal of β-carotene in the region with maximum at 500 nm was positive in the PS1 complex. The maximum value of fluorescence polarization (FP) in the measured photosynthetic pigment region was 1.25 and was similar to higher plant values. Carotenoids exhibited different efficiencies of thermal deactivation (max. at 500 nm) in PS1 and PS2. The thermal deactivation efficiency of carotenoids in comparison with that of chlorophyll (Chl) a at its red absorbance maximum was much higher in PS1 than in PS2 complexes. Cyanobacterial complexes did not contain Chl b, interpretation of the LD, PAS, and FP results is thus easier and can be compared with PS1 and PS2 values of higher plants, especially with Chl b-less mutant values.

carotenoids photoacoustic and emission spectroscopy polarized absorption reaction centres 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andersson, P.O., Gillbro, T., Asato, A.E., Liu, R.S.H.: Dual singlet state emission in a series of mini-carotenes.-J. Luminescence 51: 11-20, 1992.CrossRefGoogle Scholar
  2. Bialek-Bylka, G.E.: Model of photosynthetic pigment-protein complex.-In: Murata, N. (ed.): Research in Photosynthesis. Vol. II. Pp. 849-852. Kluwer Academic Publ., Dordrecht-Boston-London 1992.Google Scholar
  3. Bialek-Bylka, G.E., Brown, J.S.: Spectroscopy of native chlorophyll-protein complexes embedded in polyvinyl alcohol films.-Photobiochem. Photobiophys. 13: 63-71, 1986.Google Scholar
  4. Bialek-Bylka, G.E., Brown, J.S., Manikowski, H.: Active pigment-protein photosystem 1 complex in artificial matrix.-Photosynthetica 21: 182-184, 1987.Google Scholar
  5. Bialek-Bylka, G.E., Fujii, R., Chen, C.-H., Oh-oka, H., Kamiesu, A., Satoh, K., Koike, H., Koyama, Y.: 15-Cis-carotenoids found in the reaction center of green sulfur bacterium Chlorobium tepidum in the Photosystem I reaction center of a cyanobacterium Synechococcus vulcanus.-Photosynth. Res. 58:135-142, 1998.CrossRefGoogle Scholar
  6. Bialek-Bylka, G.E., Hiyama, T., Yumoto, K., Koyama, Y.: 15-Cis-β-carotene found in the reaction center of spinach Photosystem I.-Photosynth. Res. 49: 245-250, 1996.CrossRefGoogle Scholar
  7. Bialek-Bylka, G.E., Shkuropatov, A.Ya., Kadoshnikov, S.I., Frąckowiak, D.: Excitation energy transfer between β-carotene and chlorophyll a in various systems.-Photosynth. Res. 3: 241-254, 1982.CrossRefGoogle Scholar
  8. Bialek-Bylka, G.E., Sofrova, D., Skwarek, R., Kucera, T., Manikowski, H.: Energy transfer in cyanobacterial (Synechococcus elongatus) photosystem I and II.-Curr. Topics Biophys. 21: 3-7, 1997.Google Scholar
  9. Bialek-Bylka, G.E., Tomo, T., Satoh, K., Koyama, Y.: 15-cis-β-carotene found in the reaction center of spinach photosystem II.-FEBS Lett. 363: 137-140, 1995.PubMedCrossRefGoogle Scholar
  10. Biggins, J., Svejkovský, J.: Linear dichroism of microalgae, developing thylakoids and isolated pigment-protein complexes in stretched poly(vinyl alcohol) films at 77 K-Biochim. biophys. Acta 592: 565-576, 1980.PubMedCrossRefGoogle Scholar
  11. Breton, J., Vermeglio, A.: Orientation of photosynthetic pigments in vivo.-In: Govindjee (ed.): Photosynthesis. Vol. I. Pp. 153-194. Acad. Press, New York-London-Paris-San Diego-San Francisco-São Paulo-Sydney-Tokyo-Toronto 1982.Google Scholar
  12. Brody, S.S.: The position of carotene in the D-1/D-2 sub-core complex of photosystem II.-Z. Naturforsch. 43 C: 226-230, 1988.Google Scholar
  13. Brown, J.S.: A new evolution of chlorophyll absorption in photosynthetic membranes.-Photosynth. Res. 4: 375-383, 1983.Google Scholar
  14. Burton, K.S., Chow, W.S., Jordan, B.R.: The chlorophyll b-containing CP1 complexes of spinach chloroplasts.-Physiol. vég. 22: 793-800, 1984.Google Scholar
  15. Cogdell, R.J.: Carotenoids in photosynthesis.-Phil. Trans. roy. Soc. London B 284: 569-579, 1978.Google Scholar
  16. Cogdell, R.J., Frank, H.A.: How carotenoids function in photosynthetic bacteria.-Biochim. biophys. Acta 895: 63-79, 1987.PubMedGoogle Scholar
  17. Cosgrove, S.A., Guite, M.A., Burnell, T.B., Christensen R.L.: Electronic relaxation in long polyenes.-J. phys. Chem. 94: 8118-8124, 1990.CrossRefGoogle Scholar
  18. DeCoster, B., Christensen, R.L., Gebhard, R., Lugtenburg, J., Farhoosh, R., Frank, H.A.: Low-lying electronic states of carotenoids.-Biochim. biophys. Acta 1102: 107-114, 1992.PubMedCrossRefGoogle Scholar
  19. Dexter, D.L.: A theory of sensitized luminescence in solids.-J. chem. Phys. 21: 836-850, 1953.CrossRefGoogle Scholar
  20. Fiksiński, K., Frąckowiak, D.: Comparison of various films used in biophysical investigations as anisotropic matrixes.-Spectrosc. Lett. 13: 873-889, 1980.CrossRefGoogle Scholar
  21. Frank, H.A., Cogdell, R.J.: The photochemistry and function of carotenoids in photosynthesis.-In: Young, A., Britton, G. (ed.): Carotenoids in Photosynthesis. Pp. 252-326. Chapman &; Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993.Google Scholar
  22. Frank, H.A., Cogdell, R.J.: Carotenoids in photosynthesis.-Photochem. Photobiol. 63: 257-264, 1996.PubMedCrossRefGoogle Scholar
  23. Gillbro, T., Cogdell, R.J.: Carotenoid fluorescence.-Chem. Phys. Lett. 158: 312-316, 1989.CrossRefGoogle Scholar
  24. Guikema, J.A., Sherman, L.A.: Electrophoretic profiles of cyanobacterial membrane polypeptide showing heme-dependent peroxidase activity.-Biochim. biophys. Acta 637: 189-201, 1981.CrossRefGoogle Scholar
  25. Hashimoto, H., Koyama, Y.: The 21Ag-state of carotenoid bound to spinach chloroplasts as revealed by picosecond transient Raman spectroscopy.-Biochim. biophys. Acta 1017: 181-186, 1990.CrossRefGoogle Scholar
  26. Junge, W., Schaffernicht, H., Nelson, N.: On the mutual orientation of pigments in Photosystem I particles from green plants.-Biochim. biophys. Acta 462: 73-85, 1977.PubMedCrossRefGoogle Scholar
  27. Katoh, T., Nagashima, U., Mimuro, M.: Fluorescence properties of the allenic carotenoid fucoxanthin. Implication for energy transfer in photosynthetic systems.-Photosynth. Res. 27: 221-226, 1991.Google Scholar
  28. Koyama, Y.: Structures and functions of carotenoids in photosynthetic systems.-Photochem. Photobiol. B 9: 265-280, 1991.CrossRefGoogle Scholar
  29. Lichtenthaler, H.K.: Chlorophylls and carotenoids — pigments of photosynthetic membranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Acad. Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987.Google Scholar
  30. Mathis, P., Schenck, C.: The function of carotenoids in photosynthesis.-In: Britton, G., Goodwin, T.W. (ed.): Carotenoid Chemistry and Biochemistry. Pp. 339-351. Pergamon Press, Oxford-New York-Toronto-Sydney-Paris-Franfurt 1982.Google Scholar
  31. Mimuro, M., Katoh, T.: Carotenoids in photosynthesis: absorption, transfer and dissipation of light energy.-Pure appl. Chem. 63: 123-130, 1991.CrossRefGoogle Scholar
  32. Mullet, J.E., Burke, J.J., Arntzen, C.J.: A developmental study of photosystem I peripherial chlorophyll proteins.-Plant Physiol. 65: 823-827, 1980.PubMedCrossRefGoogle Scholar
  33. Noguchi, T., Kolaczkowski, S., Arbour, C., Aramaki, S., Atkinson, G.H., Hayashi, H., Tasumi, M.: Resonance Raman spectrum of the excited 21Ag state of β-carotene.-Photochem. Photobiol. 50: 603-609, 1989.CrossRefGoogle Scholar
  34. Rosencweig, A.: Photoacoustic Spectroscopy.-Wiley, New York 1980.Google Scholar
  35. Satoh, K.: Isolation and properties of the photosystem II reaction center.-In: Deisenhofer, J., Norris, J.R. (ed.).: The Photosynthetic Reaction Center. Vol. 1. Pp. 289-318. Acad. Press, San Diego-New York-Boston-London-Sydney-Tokyo-Toronto 1993.Google Scholar
  36. Shreve, A.P., Trautman, J.K., Owens, T.G., Albrecht, A.C.: Determination of the S2 lifetime of β-carotene.-Chem. Phys. Lett. 178: 89-96, 1991.CrossRefGoogle Scholar
  37. Siefermann-Harms, D.: Carotenoids in photosynthesis. I. Location in photosynthetic membranes and light-harvesting function.-Biochim. biophys. Acta 811: 325-355, 1985.Google Scholar
  38. Siefermann-Harms, D.: The light-harvesting and protective functions of carotenoids in photosynthetic membranes.-Physiol. Plant. 69: 561-568, 1987.CrossRefGoogle Scholar
  39. Siodmiak, J., Frąckowiak, D.: Polarization of fluorescence of riboflavin in anisotropic medium.-Photochem. Photobiol. 16: 173-182, 1972.PubMedCrossRefGoogle Scholar
  40. Sofrová, D., Kučera, T., Hladík, J.: Oligomeric state of cyanobacterial photosystem II.-In: Murata, N. (ed.): Research in Photosynthesis. Vol. I. Pp. 585-588. Kluwer Academic Publ., Dordrecht-Boston-London 1992.Google Scholar
  41. Szitó, T., Zimányi, L., Faludi-Dániel, A.: Fluorescence polarization spectra of granal and stromal membranes treated with linolenic acid. Orientation of the Photosystem I core complex within the membrane.-Biochim. biophys. Acta 808: 428-436, 1985.CrossRefGoogle Scholar
  42. Takahashi, Y., Koike, H., Katoh, S.: Multiple forms of chlorophyll-protein complexes from a thermophilic cyanobacterium Synechococcus sp.-Arch. Biochem. Biophys. 219: 209-218, 1982.PubMedCrossRefGoogle Scholar
  43. Tapie, P., Choquet, Y., Breton, J., Delepelaire, P., Wollman, F.-A.: Orientation of photosystem-I pigments. Investigation by low-temperature linear dichroism and polarized fluorescence emission.-Biochim. biophys. Acta 767: 57-69, 1984.CrossRefGoogle Scholar
  44. Tukaj, Z., Szurkowski, J.: Photoacoustic spectra affected by fuel oil in the chlorococcal alga Scenedesmus armatus.-Acta Physiol. Plant. 15: 219-226, 1993.Google Scholar
  45. Van Grondelle, R.: Excitation energy transfer, trapping and annihilation in photosynthetic systems.-Biochim. biophys. Acta 811: 147-195, 1985.Google Scholar
  46. Wasielewski, M.R., Kispert, L.D.: Direct measurement of the lowest excited singlet-state lifetime of all trans-β-carotene and related carotenoids.-Chem. Phys. Lett. 128: 238-243, 1986.CrossRefGoogle Scholar
  47. Young, A., Britton, G. (ed.): Carotenoids in photosynthesis.-Pp. 253-452. Chapman &; Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • G.E. Białek-Bylka
    • 1
  • D. Sofrová
    • 2
  • J. Szurkowski
    • 3
  • R. Skwarek
    • 4
  • B. Sopko
    • 2
  • H. Manikowski
    • 1
  1. 1.Faculty of Technical Physics, PoznańUniversity of TechnologyPoznań, Piotrowo Str. 3Poland
  2. 2.Biochemistry Department, Faculty of ScienceCharles UniversityPrague, AlbertovCzech Republic
  3. 3.Institute of PhysicsUniversity of GdanskPoland
  4. 4.Faculty of Technical Physics, PoznańUniversity of TechnologyPoznań, Piotrowo Str. 3Poland

Personalised recommendations