, Volume 38, Issue 1, pp 143–148 | Cite as

Linear Dichroism, Fluorescence Polarization, and Path of the Thermal Deactivation of Excited Cyanobacterial (Synechococcus Elongatus) Photosystem 1 Immobilized and Oriented in Polymer Films

  • G.E. Białek-Bylka
  • D. Sofrová
  • J. Szurkowski
  • R. Skwarek
  • B. Sopko
  • H. Manikowski


Pigment-protein complexes enriched in photosystem 1 (PS1) and, for comparison, enriched in photosystem 2 (PS2) were isolated from the cyanobacterium Synechococcus elongatus Nag. f. thermalis Geitl. They were immobilized and oriented in the polyvinyl alcohol (PVA) films, and studied by linear dichroism (LD), fluorescence polarization (FP), photoacoustic spectroscopy (PAS), and polarized photoacoustic spectroscopy (PAS and PAS). The LD signal of β-carotene in the region with maximum at 500 nm was positive in the PS1 complex. The maximum value of fluorescence polarization (FP) in the measured photosynthetic pigment region was 1.25 and was similar to higher plant values. Carotenoids exhibited different efficiencies of thermal deactivation (max. at 500 nm) in PS1 and PS2. The thermal deactivation efficiency of carotenoids in comparison with that of chlorophyll (Chl) a at its red absorbance maximum was much higher in PS1 than in PS2 complexes. Cyanobacterial complexes did not contain Chl b, interpretation of the LD, PAS, and FP results is thus easier and can be compared with PS1 and PS2 values of higher plants, especially with Chl b-less mutant values.

carotenoids photoacoustic and emission spectroscopy polarized absorption reaction centres 


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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • G.E. Białek-Bylka
    • 1
  • D. Sofrová
    • 2
  • J. Szurkowski
    • 3
  • R. Skwarek
    • 4
  • B. Sopko
    • 2
  • H. Manikowski
    • 1
  1. 1.Faculty of Technical Physics, PoznańUniversity of TechnologyPoznań, Piotrowo Str. 3Poland
  2. 2.Biochemistry Department, Faculty of ScienceCharles UniversityPrague, AlbertovCzech Republic
  3. 3.Institute of PhysicsUniversity of GdanskPoland
  4. 4.Faculty of Technical Physics, PoznańUniversity of TechnologyPoznań, Piotrowo Str. 3Poland

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