Biotechnology Letters

, Volume 23, Issue 1, pp 55–60

Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze

  • Hye Kyong Kim
  • Sei-Ryang Oh
  • Hyeong-Kyu Lee
  • Hoon Huh


Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g−1 dry wt) during the stationary phase of culture, after 18 days of inoculation. The addition of yeast extract preparation (MW < 10 000) at 50 μg ml−1 elevated the rosmarinic acid content up to 5.7-fold of that found in non-elicited suspension cells. The elicitation of yeast extract preparation was further 2-fold enhanced by the presence of benzothiadiazole, a synthetic activator of plant systemic acquired resistance, as compared to yeast extract alone. These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures.

Agastache rugosa benzothiadiazole rosmarinic acid suspension culture yeast elicitor 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Hye Kyong Kim
    • 1
  • Sei-Ryang Oh
    • 2
  • Hyeong-Kyu Lee
    • 2
  • Hoon Huh
    • 1
  1. 1.Research Institute of Pharmaceutical Sciences, College of PharmacySeoul National UniversitySeoulKorea
  2. 2.Korea Research Institute of Bioscience and Biotechnology, KISTTaejonKorea

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