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Glycoconjugate Journal

, Volume 17, Issue 3–4, pp 205–214 | Cite as

Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm

  • Kaoru Ohta
  • Chihiro Sato
  • Tsukasa Matsuda
  • Masaru Toriyama
  • Victor D. Vacquier
  • William J. Lennarz
  • Ken Kitajima
Article

Abstract

The low density, detergent-insoluble membrane fraction (LD-DIM), where gangliosides are likely to be highly enriched, was prepared from sperm of two sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus. Immunoblotting showed the presence in the LD-DIM of two receptors for egg ligands, a glycosylphosphatidylinositol (GPI)-anchored protein, and four proteins which may be involved in signal transduction. Co-immunoprecipitation revealed that at least three proteins, the speract receptor, the 63[emsp4 ]kDa GPI-anchored protein and the α subunit of a heterotrimeric Gs protein, are localized in the LD-DIM. This suggests that the LD-DIM fraction may be a membrane microdomain for speract–speract receptor interaction, as well as the subsequent signal transduction pathway involved in induction of sperm respiration, motility and possibly the acrosome reaction.

sea urchin sperm ganglioside detergent-insoluble membrane signal transduction fertilization acrosome reaction 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Kaoru Ohta
    • 1
  • Chihiro Sato
    • 1
  • Tsukasa Matsuda
    • 1
  • Masaru Toriyama
    • 2
  • Victor D. Vacquier
    • 3
  • William J. Lennarz
    • 4
  • Ken Kitajima
    • 1
  1. 1.Department of Applied Molecular Biosciences, Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan
  2. 2.Department of Applied Biological Chemistry, Faculty of AgricultureShizuoka University, OhyaShizuokaJapan
  3. 3.Scripps Institution of OceanographyUniversity of California, San DiegoLa JollaU.S.A
  4. 4.Department of Biochemistry and Cell BiologyState University of New York at Stony BrookNew YorkU.S.A

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