Conservation Genetics

, Volume 1, Issue 2, pp 163–168

Cross-species amplification of microsatellite primers in passerine birds

  • Peter Galbusera
  • Stefan van Dongen
  • Erik Matthysen


Developing species specific microsatellite primers can be avoidedby using existing markers which amplify across species. However,for passerines, such ‘cross-species’ markers are mostly lackingand few guidelines exist for selecting them from the wide rangeof existing markers. Here cross-species amplification tests of 40microsatellite primers in 13 passerine species show an increasein probability of amplification and polymorphism with decreasingphylogenetic distance. Primers which successfully amplified inmany species had a higher chance to be polymorphic. However,since the amplification success, across a broad range of species,of particular primersets remains difficult to predict it iscrucial to identify such markers empirically. Here we describesuch widely applicable bird (passerines) microsatellite markers.

cross-amplification microsatellite passerine 


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Copyright information

© Springer Science+Business Media Dordrecht 2000

Authors and Affiliations

  • Peter Galbusera
    • 1
  • Stefan van Dongen
    • 2
  • Erik Matthysen
    • 2
  1. 1.Laboratory of Animal Ecology, Department of BiologyUniversity of Antwerp (UIA)WilrijkBelgium
  2. 2.Laboratory of Animal Ecology, Department of BiologyUniversity of Antwerp (UIA)WilrijkBelgium

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