Conservation Genetics

, Volume 1, Issue 2, pp 163–168

Cross-species amplification of microsatellite primers in passerine birds

  • Peter Galbusera
  • Stefan van Dongen
  • Erik Matthysen
Article

Abstract

Developing species specific microsatellite primers can be avoidedby using existing markers which amplify across species. However,for passerines, such ‘cross-species’ markers are mostly lackingand few guidelines exist for selecting them from the wide rangeof existing markers. Here cross-species amplification tests of 40microsatellite primers in 13 passerine species show an increasein probability of amplification and polymorphism with decreasingphylogenetic distance. Primers which successfully amplified inmany species had a higher chance to be polymorphic. However,since the amplification success, across a broad range of species,of particular primersets remains difficult to predict it iscrucial to identify such markers empirically. Here we describesuch widely applicable bird (passerines) microsatellite markers.

cross-amplification microsatellite passerine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bensch S, Price T, Kohn J (1997) Isolation and characterization of microsatellite loci in a Phylloscopus warbler. Mol. Ecol., 6, 91-92.CrossRefPubMedGoogle Scholar
  2. Brooks T, Lens L, Barnes J, Barnes R, Kihuria JK, Wilder C (1998) The conservation status of the forest birds of the Taita Hills, Kenya. Bird Conservation International, 8, 119-139.CrossRefGoogle Scholar
  3. Dallimer M (1999) Cross-species amplification success of avian microsatellites in the redbilled quelea Quelea quelea. Mol. Ecol., 8, 685-702.CrossRefGoogle Scholar
  4. Dawson RJG, Gibbs HL, Hobson KA, Yzerinac SM (1997) Isolation of microsatellite DNA markers from a passerine bird, Dendroica petechia (the yellow warbler), and their use in population studies. Heredity, 79, 506-514.CrossRefPubMedGoogle Scholar
  5. Degnan SM, Robertson BC, Clegg SM, Moritz CC (1999) Microsatellite primers for studies of gene flow and mating systems in white-eyes (Zosterops). Mol. Ecol., 8, 159-160.CrossRefPubMedGoogle Scholar
  6. Double MC, Dawson D, Burke T, Cockburn A (1997) Finding the fathers in the least faithful bird: a microsatellite-based genotyping system for the superb fairy-wren Malurus cyaneus. Mol. Ecol., 6, 691-693.CrossRefGoogle Scholar
  7. Estoup A, Solignac M, Harry M, Cornuet J-M (1993) Characterization of (GT)n and (CT)n microsatellites in two insect species: Apis mellifera and Bombus terrestris. Nucleic Acids Research, 21, 1427-1431.CrossRefPubMedPubMedCentralGoogle Scholar
  8. Field RL, Scribner KT (1997) Isolation and characterization of novel waterfowl microsatellite loci: cross-species comparisons and research applications. Mol. Ecol., 6, 199-202.CrossRefGoogle Scholar
  9. Fridolfsson A, Gyllensten UB, Jakobsson S (1997) Microsatellite markers for paternity testing in the Willow warbler Phylloscopus trochilus: high frequency of extra-pair young in an island population. Hereditas, 126, 127-132.CrossRefGoogle Scholar
  10. Galbusera P, Lens L, Waiyaki E, Schenck T, Matthysen E (2000) Genetic variability and gene flow in the globally, criticallyendangered Taita thrush. Conservation Genetics, 1, 45-55.CrossRefGoogle Scholar
  11. Griffiths R, Double MCY, Orr K, Dawson RJG (1998) A DNA test to sex most birds. Mol. Ecol., 7, 1071-1075.CrossRefPubMedGoogle Scholar
  12. Griffith SC, Stewart IRK, Dawson DA, Owens IPF, Burke T (1999) Contrasting levels of extra-pair paternity in mainland and island populations of the house sparrow (Passer domesticus): is there an ‘island effect’? Biological Journal of the Linnean Society, 68, 303-316.Google Scholar
  13. Hughes CR, Kavlie R, Johnson K (1998) Characterization of polymorphic trinucleotide microsatellite loci in the great-tailed grackle, Quiscalus mexicanus. Mol. Ecol., 7, 783-784.CrossRefGoogle Scholar
  14. Ishibashi Y, Mikami O, Abe S (2000) Isolation and characterization of microsatellite loci in the Japanese marsh warbler Locustella pryeri. Mol. Ecol., 9, 373-375.CrossRefPubMedGoogle Scholar
  15. Jeffrey KJ, Keller LF, Arcese P, Bruford MW (2000) The development of microsatellite loci in the song sparrow (Melospiza melodia) and genotyping errors associated with good quality DNA. Mol. Ecol. (in press).Google Scholar
  16. Kashi Y, Soller M (1999) Functional roles of microsatellites and minisatellites. In: Microsatellites: Evolution and Applications (eds. Goldstein B, Schlötterer C) pp. 10-23. Oxford University Press, New York.Google Scholar
  17. Li S-H, Yi-Jiun H, Brown JL (1997) Isolation of tetranuleotide microsatellites from the Mexican jay Aphelocoma ultramarina. Mol. Ecol., 6, 499-501.CrossRefPubMedGoogle Scholar
  18. Littell RC, Milliken GA, Stroup WW, Wolfinger RD (1996) SASr System for Mixed Models. SAS Institute Inc., New York.Google Scholar
  19. Martinez JG, Soler JJ, Soler M, Miller AP, Burke T (1999) Comparative population structure and gene flow of a brood parasite, the great spotted cuckoo (Clamator glandarius), and its primary host, the magpie (Pica pica). Evolution, 53, 269-278.CrossRefGoogle Scholar
  20. McDonald DB, Potts W (1994) Cooperative display and relatedness among males in a lek-mating bird. Science, 266, 1030-1032.CrossRefPubMedGoogle Scholar
  21. Moore SS, Sargeant LL, King TJ (1991) The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics, 10, 654-660.CrossRefPubMedGoogle Scholar
  22. Mundy NI, Woodruff DS (1996) Polymorphic microsatellite markers in the loggerhead shrike Lanius ludovicianus isolated from a library enriched for CA repeats. Mol. Ecol., 5, 811-814.CrossRefPubMedGoogle Scholar
  23. Neumann K, Wetton JH (1996) Highly polymorphic microsatellites in the house sparrow Passer domesticus. Mol. Ecol., 5, 307-309.CrossRefPubMedGoogle Scholar
  24. Painter J, Crozier RH, Crozier YC, Clarke MF (1997) Characterization of microsatellite loci for a co-operatively breeding honeyeater. Mol. Ecol., 6, 1103-1105.CrossRefPubMedGoogle Scholar
  25. Petren K (1998) Microsatellite primers from Geospiza fortis and cross-species amplification in Darwin's finches. Mol. Ecol., 7, 1771-1788.CrossRefGoogle Scholar
  26. Piertney SB, Marquiss M, Summers R (1998) Characterization of tetranucleotide microsatellite markers in the Scottish crossbill (Loxia scotica). Mol. Ecol., 7, 1247-1263.CrossRefGoogle Scholar
  27. Primmer CR, Møller AP, Ellegren H (1995) Resolving genetic relationships with microsatellite markers: a parentage testing system for the swallow Hirundo rustica. Mol. Ecol., 4, 493-498.CrossRefPubMedGoogle Scholar
  28. Primmer CR, Møller AP, Ellegren H (1996) A wide-range survey of cross-species microsatellite amplification in birds. Mol. Ecol., 5, 365-378.CrossRefPubMedGoogle Scholar
  29. Primmer CR, Raudsepp T, Chowdhary BP, Moller AP, Ellegren H (1997) Low frequency of microsatellites in the avian genome. Genome Research, 7, 471-482.PubMedGoogle Scholar
  30. Scribner KT, Pearce JM (2000) Microsatellites: evolutionary and methodological background and empirical applications at individual, population, and phylogenetic levels. In: Molecular Methods in Ecology (ed. Baker A), pp. 235-271. Blackwell Science Limited, London, England.Google Scholar
  31. Sibley CG, Ahlquist JE (1990) Phylogeny and Classification of Birds: A study in Molecular Evolution. Yale University Press, New Haven.Google Scholar
  32. Verbeke G, Molenberghs G (1997) Linear Mixed Models in Practice: A SAS Oriented Approach. Lecture notes in statistics, Vol. 126. Springer, New York.Google Scholar
  33. Weber JL (1990) Informativeness of human (dC-dA)n.(dG-dT)n polymorphisms. Genomics, 7, 524-530.CrossRefPubMedGoogle Scholar
  34. Zardoya R, Vollmer DM, Craddock C, Streelman JT, Karl S, Meyer A (1996) Evolutionary conservation of microsatellite flanking regions and their use in resolving the phylogeny of cichlid fishes (Pisces: Perciformes). Proc. Royal Soc. London B, 263, 1589-1598.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2000

Authors and Affiliations

  • Peter Galbusera
    • 1
  • Stefan van Dongen
    • 2
  • Erik Matthysen
    • 2
  1. 1.Laboratory of Animal Ecology, Department of BiologyUniversity of Antwerp (UIA)WilrijkBelgium
  2. 2.Laboratory of Animal Ecology, Department of BiologyUniversity of Antwerp (UIA)WilrijkBelgium

Personalised recommendations