Cross-species amplification of microsatellite primers in passerine birds
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Developing species specific microsatellite primers can be avoidedby using existing markers which amplify across species. However,for passerines, such ‘cross-species’ markers are mostly lackingand few guidelines exist for selecting them from the wide rangeof existing markers. Here cross-species amplification tests of 40microsatellite primers in 13 passerine species show an increasein probability of amplification and polymorphism with decreasingphylogenetic distance. Primers which successfully amplified inmany species had a higher chance to be polymorphic. However,since the amplification success, across a broad range of species,of particular primersets remains difficult to predict it iscrucial to identify such markers empirically. Here we describesuch widely applicable bird (passerines) microsatellite markers.
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- Griffith SC, Stewart IRK, Dawson DA, Owens IPF, Burke T (1999) Contrasting levels of extra-pair paternity in mainland and island populations of the house sparrow (Passer domesticus): is there an ‘island effect’? Biological Journal of the Linnean Society, 68, 303-316.Google Scholar
- Jeffrey KJ, Keller LF, Arcese P, Bruford MW (2000) The development of microsatellite loci in the song sparrow (Melospiza melodia) and genotyping errors associated with good quality DNA. Mol. Ecol. (in press).Google Scholar
- Kashi Y, Soller M (1999) Functional roles of microsatellites and minisatellites. In: Microsatellites: Evolution and Applications (eds. Goldstein B, Schlötterer C) pp. 10-23. Oxford University Press, New York.Google Scholar
- Littell RC, Milliken GA, Stroup WW, Wolfinger RD (1996) SASr System for Mixed Models. SAS Institute Inc., New York.Google Scholar
- Scribner KT, Pearce JM (2000) Microsatellites: evolutionary and methodological background and empirical applications at individual, population, and phylogenetic levels. In: Molecular Methods in Ecology (ed. Baker A), pp. 235-271. Blackwell Science Limited, London, England.Google Scholar
- Sibley CG, Ahlquist JE (1990) Phylogeny and Classification of Birds: A study in Molecular Evolution. Yale University Press, New Haven.Google Scholar
- Verbeke G, Molenberghs G (1997) Linear Mixed Models in Practice: A SAS Oriented Approach. Lecture notes in statistics, Vol. 126. Springer, New York.Google Scholar