Plant and Soil

, Volume 226, Issue 2, pp 131–151 | Cite as

Mycelium of Arbuscular Mycorrhizal fungi (AMF) from different genera: form, function and detection

  • John C. Dodd
  • Claire L. Boddington
  • Alia Rodriguez
  • Carmen Gonzalez-Chavez
  • Irdika Mansur

Abstract

It is often assumed that all species of arbuscular mycorrhizal fungi (AMF) have the same function because of the ubiquity of the arbuscular mycorrhizal symbiosis and the fact that all AMF occupy the same plant/soil niche. Despite apparent differences in the timing of evolutionary divergence and the morphological characteristics of AMF from the different genera, the majority of studies on these fungi use only species of Glomus. There is increasing evidence, however, that the mechanisms involved in the establishment of a mycorrhiza may differ for species and genera of AMF and influence their subsequent function. The aim of this paper is to highlight the diversity in the form and function of AMF from different genera, knowledge of which is vital in understanding their ecological roles. Potential use of biochemical and molecular approaches to detect AMF in planta and ex planta is also discussed.

arbuscular mycorrhizas ecological function isozymes morphology mycelium architecture molecular probes 

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References

  1. Abbott L K 1982 Comparative anatomy of vesicular-arbuscular mycorrhizas formed on subterranean clover. Aust. J. Bot. 30, 485–499.CrossRefGoogle Scholar
  2. Abbott L K and Gazey C 1994 An ecological view of the formation of VA mycorrhizas. Plant Soil 159, 69–78.Google Scholar
  3. Abbott L K and Robson A D 1985 Formation of external hyphae in soil by four species of vesicular-arbuscular mycorrhizal fungi. New Phytol. 99, 245–255.CrossRefGoogle Scholar
  4. Abbott L K, Robson A D, Jasper D A and Gazey C 1992What is the role of VA Mycorrhizal Hyphae in soil? In Mycorrhizas in Ecosystems. Eds. DJ Read, DH Lewis, AH Fitter and IJ Alexander. pp 37–41. CAB International, University Press Cambridge.Google Scholar
  5. Addy H D, Miller M H and Peterson R L 1997 Infectivity of the propagules associated with extraradical mycelia of two AMfungi following winter freezing. New Phytol. 135, 745–753.CrossRefGoogle Scholar
  6. Alexander T, Toth R, Meier R and Weber H C 1989 Dynamics of arbuscule development and degeneration in onion, bean and tomato with particular reference to vesicular-arbuscular mycorrhizae in grasses. Can. J. Bot. 67, 2505–2513.Google Scholar
  7. Allen MF 1996 The ecology of arbuscular mycorrhizas: a look back into the 20th century and a peek into the 21st. Mycol. Res. 100, 769–782.Google Scholar
  8. Ames R N, Ingham E R and Reid C P P 1982 Ultraviolet-induced autofluorescence of arbuscular mycorrhizal root infections: an alternative to cleaning and staining methods for assessing infections. Can. J. Microbiol. 28, 351–355.CrossRefGoogle Scholar
  9. Amijee F, Tinker P B and Stribley D P 1989 The development of endomycorrhizal root systems. VII. A detailed study of effects of soil phosphorus on colonisation. New Phytol. 111, 435–446.CrossRefGoogle Scholar
  10. Amijee F, Stribley D P and Lane P W 1993 The susceptibility of roots to infection by an arbuscular mycorrhizal fungus in relation to age and phosphorus supply. New Phytol. 125, 581–586.CrossRefGoogle Scholar
  11. Bago B, Azcon-Aguilar C and Piche Y 1998 Architecture and developmental dynamics of the external mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown under monoaxenic conditions. Mycologia 90, 52–62.Google Scholar
  12. BEG 1999 - WWW page URL: wwwbio.ukc.ac.uk/begGoogle Scholar
  13. Bentivenga S P and Morton J B 1995 A monograph of the genus Gigaspora, incorporating developmental patterns of morphological characters. Mycologia 87, 719–731.Google Scholar
  14. Boddington C L and Dodd J C 1998 A comparison of the development and metabolic activity of mycorrhizas formed by arbuscular mycorrhizal fungi from different genera on two tropical forage legumes. Mycorrhiza 8, 149–157.CrossRefGoogle Scholar
  15. Boddington C L and Dodd J C 1999 Evidence that differences in phosphate metabolism in mycorrhizas formed by species of Glomus and Gigaspora may be related to their life-cycle strategies. New Phytol.Google Scholar
  16. Boddington C L, Bassett E E, Jakobsen I and Dodd J C 1999 Comparison of techniques for the extraction and quantification of extra-radical mycelium of arbuscular mycorrhizal fungi from two soils containing fine particulate matter. Soil Biol. Biochem. 31, 479–482.CrossRefGoogle Scholar
  17. Braunberger P G, Abbott L K and Robson A D 1994 The effect of rain in the dry season on the formation of vesicular-arbuscular mycorrhizas in the growing season of annual clover-based pastures. New Phytol. 127, 107–114.CrossRefGoogle Scholar
  18. Brundrett M C, Piche Y and Peterson R L 1984 A new method for observing the morphology of vesicular-arbuscular mycorrhizae. Can. J. Bot. 62, 2128–2134.Google Scholar
  19. Brundrett M and Kendrick B 1988 The mycorrhizal status, root anatomy and phenology of plants in a sugar maple forest. Can. J. Bot. 66, 1153–1173.Google Scholar
  20. Brundrett M and Kendrick B 1990 The roots and mycorrhizae of herbaceous woodland plants. II. Structural aspects of morphology. New Phytol. 114, 469–479.CrossRefGoogle Scholar
  21. Brundrett M and Abbott L K 1994 Mycorrhizal fungus propagules in the jarrah forest I. Seasonal study of inoculum levels. New Phytol. 127, 539–546.CrossRefGoogle Scholar
  22. Brundrett M, Bougher N, Dell B, Grove T and Malajczuk N 1996 Working with Mycorrhizas in Forestry and Agriculture, ACIAR Monograph 32. 373 p.Google Scholar
  23. Claassen V P, Zasoski R J and Tyler B M 1996 A method for direct soil extraction and PCR amplification of endomycorrhizal fungal DNA. Mycorrhiza 6, 447–450.CrossRefGoogle Scholar
  24. Clapp J P, Young J P W, Merryweather J and Fitter A H 1995 Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community. New Phytol. 130, 259–265.CrossRefGoogle Scholar
  25. Clapp J P, Fitter A H and Young J P W 1999 Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp. Molecular Ecology 8, 915–922.PubMedCrossRefGoogle Scholar
  26. Daniels-Hetrick B A, Bloom J and Feyerherm S M 1985 Root colonization pattern of Glomus epigaeum in nine host species. Mycologia 77, 825–828.Google Scholar
  27. Declerck S, Strullu D G and Plenchette C 1996 In vitro massproduction of the arbuscular mycorrhizal fungus, Glomus versiforme, associated with Ri T-DNA transformed carrot roots. Mycol. Res. 100, 1237–1242.Google Scholar
  28. Di Bonito R, Elliot M L and Des Jardin E A 1995 Detection of an arbuscular mycorrhizal fungus in roots of different plant species with PCR. Appl. Environ Microbiol. 61, 2809–2810.PubMedGoogle Scholar
  29. Dodd J C 1994 Approaches to the study of the extraradical mycelium of arbuscular mycorrhizal fungi. In Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystems. Eds. S Gianinazzi and H Schüepp. pp 147-166. Birkhäuser Verlag Basel, Switzerland.Google Scholar
  30. Dodd J C, Arias I, Koomen I and Hayman D S 1990 The management of vesicular-arbuscular mycorrhizal populations in acidinfertile soils of a savanna ecosystem. I. The effect of precropping and VAMF inoculation on plant growth and nutrition in the field. Plant Soil 122, 229–240.Google Scholar
  31. Edwards S G, Fitter A H and Young J P W 1997 Identification of an arbuscular mycorrhizal fungus, Glomus mosseae, within plant roots by competitive polymerase chain reaction. Mycol. Res. 101, 1440–1444.CrossRefGoogle Scholar
  32. Ezawa, T, Kuwahara S, Sakamoto K, Yoshida T and Saito M 1999 Specific inhibitor and substrate specificity of alkaline phosphatase expressed in the symbiotic phase of the arbuscular mycorrhizal fungus, Glomus etunicatum. Mycologia 91, 636–641.Google Scholar
  33. Friese C F and Allen M F 1991 The spread of VA mycorrhizal fungi hyphae in the soil: inoculum types and external hyphal structure. Mycologia 83, 409–418.Google Scholar
  34. Gange A C, Bower E, Stagg P G, Aplin D M, Gillam A E and Bracken M 1999 A comparison of visualization techniques for recording arbuscular mycorrhizal colonization. New Phytol. 142, 123–132.CrossRefGoogle Scholar
  35. Gazey C, Abbott L K and Robson A D 1992 The rate of development of mycorrhizas affects the onset of sporulation and production of external hyphae by two species of Acaulospora. Mycol. Res. 96, 643–550.Google Scholar
  36. Gazey C, Abbott L K and Robson A D 1993 VA mycorrhizal spores from three species of Acaulospora: germination, longevity and hyphal growth. Mycol. Res. 97, 785–790.Google Scholar
  37. Graham J H, Hodge N C and Morton, J B 1995 Fatty acid methyl ester profiles for characterisation of Glomalean fungi and their endomycorrhizae. Appl. Environ. Microbiol. 61, 58–64.PubMedGoogle Scholar
  38. Graham J H, Duncan L W and Eissenstat D M 1997 Carbohydrate allocation patterns in citrus genotypes as affected by phosphorus nutrition, mycorrhizal colonization and mycorrhizal dependency. New Phytol. 135, 335–343.CrossRefGoogle Scholar
  39. Harrison M J 1998 Development of the arbuscular mycorrhizal symbiosis. Current Opinion in Plant Biology 1, 360–365.PubMedCrossRefGoogle Scholar
  40. Helgason T, Daniell T J, Husband R, Fitter A H and Young J P W 1998 Ploughing up the wood-wide web? Nature 394, 431.PubMedCrossRefGoogle Scholar
  41. Hepper C M 1985 Influence of age of roots on the pattern of vesicular arbuscular mycorrhizal infection in leek and clover New Phytol. 101, 685–693.Google Scholar
  42. Hepper C M, Sen R and Maskall C S 1986. Identification of vesicular-arbuscular mycorrhizal fungi in roots of leek (Allium porrum L.) and maize (Zea mays L.) on the basis of enzyme mobility during polyacrylamide gel electrophoresis. New Phytol. 102, 529–539.CrossRefGoogle Scholar
  43. Hepper C M, Azcon-Aguilar C, Rosendahl S and Sen R 1988 Competition between three species of Glomus used as spatially separated introduced and indigenous mycorrhizal inoculation for leek (Allium porrum L.). New Phytol. 110, 207–215.CrossRefGoogle Scholar
  44. INVAM 1999 WWWpage URL: http V ==invam:caf:wvu:edu= Google Scholar
  45. Jakobsen I, Abbott L K and Robson A D 1992a External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 1. Spread of hyphae and phosphorus inflow into roots. New Phytol. 120, 371–380.CrossRefGoogle Scholar
  46. Jakobsen I, Abbott L K and Robson A D 1992b External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Trifolium subterraneum L. 2. Hyphal transport of 32P over defined distances. New Phytol. 120, 509–516.CrossRefGoogle Scholar
  47. Jasper D A, Robson A D and Abbott L K 1988 Revegetation in an iron-ore mine - nutrient requirements for plant growth and the potential role of vesicular arbuscular (VA) mycorrhizal fungi. Aust. J. Soil Res. 26, 497–507.CrossRefGoogle Scholar
  48. Johnson N C, Graham J H and Smith F A 1997 Functioning of mycorrhizal associations along the mutualism-parasitism continuum. New Phytol. 135, 575–585.CrossRefGoogle Scholar
  49. Kjoller R and Rosendahl S. 1996 Arbuscular mycorrhizal fungi in roots from a Danish pea field determined by polyacrylamide gel electrophoresis of specific fungal enzymes. Abstract of oral presentation at 1st International Conference onMycorrhizae, San Francisco, CA, USA. July 31- August 3 1996.Google Scholar
  50. Kjoller R and Rosendahl S 2000 - Plant Soil 226, 189–196.CrossRefGoogle Scholar
  51. Klironimos J N and Kendrick B 1993 Research on mycorrhizas: trends in the past 40 years as expressed in the 'MYCOLIT' database. New Phytol. 125, 595–600.CrossRefGoogle Scholar
  52. Kormanik P P and McGraw A-C 1982 Quantification of vesiculararbuscular mycorrhizae in plant roots. In Methods and Principles of Mycorrhizal Research. Ed. NC Schenck. pp 37–45. St. Paul, MN, USA. American Phytopathological Society.Google Scholar
  53. Lanfranco L, Delpero M and Bonfante P 1999 Intrasporal variability of ribosomal sequences in the endomycorrhizal fungus Gigaspora margarita. Molecular Ecology 8, 37–45.PubMedCrossRefGoogle Scholar
  54. Larsen J, Olsson P A and Jakobsen I 1998 The use of fatty acid signatures to study mycelial interactions between the arbuscular mycorrhizal fungus Glomus intraradices and the saprotrophic fungus Fusarium culmorum in root-free soil. Mycol. Res. 102, 1491–1496.CrossRefGoogle Scholar
  55. Lee P-J and Koske R E Gigaspora gigantea: seasonal abundance and ageing of spores in a sand dune. Mycol. Res. 98, 453- 457.Google Scholar
  56. Lloyd-Macgilp S A, Chambers S M, Dodd J C, Fitter A H, Walker C and Young J P W 1996 Diversity of the internal transcribed spacers within and among isolates of Glomus mosseae and related arbuscular mycorrhizal fungi. New Phytol. 133, 103–112.CrossRefGoogle Scholar
  57. Merryweather J and Fitter A 1991 A modified method for elucidating the structure of the fungal partner in a vesicular-arbuscular mycorrhiza. Mycol. Res. 95, 1435–1437.CrossRefGoogle Scholar
  58. Merryweather J and Fitter A 1998 The arbuscular mycorrhizal fungi of Hyacinthoides non-scripta. I. Diversity of fungal taxa. New Phytol. 138, 117–129.CrossRefGoogle Scholar
  59. Miller R M and Jastrow J D 1992 The role of mycorrhizal fungi in soil conservation. In Proceedings of a Symposium on Mycorrhizae in Sustainable Agriculture. Eds. GJ Bethlenfalvay and RG Linderman. pp 29-44. ASA Special Publication No. 54. Madison, Wisconsin, USA.Google Scholar
  60. Morton J B 1985 Underestimation of most probable numbers of vesicular arbuscular endophytes because of non-staining mycorrhizae. Soil Biol. Biochem. 17, 383–384.CrossRefGoogle Scholar
  61. Morton J B and Benny G L 1990 Revised classification of arbuscular mycorrhizal fungi (Zygomycetes): a new order, Glomales, two new suborders, Glomineae and Gigasporineae, and two new families, Acaulosporaceae and Gigasporinaceae, with an emendation of Glomaceae. Mycotaxon 37, 471–491.Google Scholar
  62. Morton J B and Bentivenga S P 1994 Levels of diversity in endomycorrhizal fungi (Glomales, Zygomycetes) and their role in defining taxonomic and non-taxonomic groups. Plant Soil 159, 47–59.Google Scholar
  63. Morton J B 1995 Taxonomic and phylogenetic divergence among five Scutellospora species based on comparative developmental sequences. Mycologia 87, 127–137.Google Scholar
  64. Nicolson T H 1959 Mycorrhiza in the Graminae. I. Vesicular- arbuscular endophytes, with special reference to the external phase. Trans. Brit. Mycol. Soc. 42, 421–438.Google Scholar
  65. Olsson P A, Baath E, Jakobsen I and Soderstrom B 1995 The use of phospholipid and neutral fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil. Mycol. Res. 99, 623–629.Google Scholar
  66. Pearson J N and Jakobsen I 1993 Symbiotic exchange of carbon and phosphorus between cucumber and three arbuscular mycorrhizal fungi. New Phytol. 124, 481–488.CrossRefGoogle Scholar
  67. Pearson J N and Schweiger P 1993 Scutellospora calospora (Nicol. And Gerd.) Walker and Sanders associated with subterranean clover: dynamics of colonisation, sporulation and soluble carbohydrates. New Phytol. 124, 215–219.CrossRefGoogle Scholar
  68. Pearson J N, Abbott L K and Robson A D 1993 Mediation of competition between two colonising VA mycorrhizal fungi by the host plant. New Phytol. 123, 93–98.CrossRefGoogle Scholar
  69. Phillips J M and Hayman, D S 1970. Improved procedure for clearing roots and staining parasitic and vesicular arbuscular fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 55, 158–161.CrossRefGoogle Scholar
  70. Read D 1998 Plants on the Web. Nature 396, 22–23.CrossRefGoogle Scholar
  71. Riling M C, Wright S F, Allen M F and Field C B 1999 Rise in carbon dioxide changes soil structure. Nature 400, 628.CrossRefGoogle Scholar
  72. Rivillas C A 1995 The effects of arbuscular mycorrhizal fungi on two different coffee varieties from Colombia and their biochemical detection in roots. M.Sc. Thesis, University of Kent at Canterbury, UK. 87 p.Google Scholar
  73. Rivillas C and Dodd J C 1996 The effects of arbuscular mycorrhizal fungi on two different coffee varieties from Colombia and their biochemical detection in roots. InMycorrhizas in Integrated Systems from Genes to Plant Development. Proceedings of 4th European Symposium on Mycorrhizas, Granada, Spain 11- 14 July 1994. pp 47–50. EC Brussels.Google Scholar
  74. Rodriguez A and Dodd J C 1998 The use of isozyme markers to screen the intraspecific diversity of isolates of Glomus etunicatum in comparison with other species of Glomus. Abstract poster presented at ICOM2 in Uppsala, Sweden 1998.Google Scholar
  75. Rouhier H and Read D J 1998 The role of mycorrhiza in determining the response of Plantago lanceolata to CO2 enrichment. New Phytol. 139, 367–373.CrossRefGoogle Scholar
  76. Rozycka M 1994 Use of biochemical and immunological methods to distinguish arbuscular mycorrhizal fungi. Ph.D. Thesis, University of Kent at Canterbury, UK. 160 p.Google Scholar
  77. Sanders F E, Mosse B and Tinker PB (eds) 1975 Endomycorrhizas. Academic Press, London, UK. 626 p.Google Scholar
  78. Sanders I R, Alt M, Groppe K, Boller T and Wiemken A 1995 Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytol. 130, 419–427.CrossRefGoogle Scholar
  79. Sanders I R, Clapp J P and Wiemken A 1996 The genetic diversity of arbuscular mycorrhizal fungi in natural ecosystems - a key to understanding the ecology and functioning of the mycorrhizal symbiosis. New Phytol. 133, 123–134.CrossRefGoogle Scholar
  80. Sanders I R, Streitwolf-Engel R, Van der Heijden M G A, Boller T and Wiemken A 1998 Increased allocation to external hyphae of arbuscular mycorrhiza fungi under CO2 enrichment. Oecologia 117, 496–503.CrossRefGoogle Scholar
  81. Simon L, Lalonde M and Bruns T 1992 Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl. Environ. Microbiol. 58, 291–295.PubMedGoogle Scholar
  82. Simon L, Bousquet J, Lè vesque R C and Lalonde M 1993a Origin and diversification of endomycorrhizal fungi and coincidence with vascular land plants. Nature 363, 67–69.CrossRefGoogle Scholar
  83. Simon L, Vesque R and Lalonde M 1993b Identification of endomycorrhizal fungi colonizing roots by fluorescent single-strand conformation polymorphism-polymerase chain reaction. Appl. Environ. Microbiol. 59, 4211–4215.PubMedGoogle Scholar
  84. Smith F A and Smith S E 1995 Nutrient transfer in vesiculararbuscular mycorrhizas: a new model based on the distribution of ATPases on fungal and plant membranes. Biotropia 8, 1–10.Google Scholar
  85. Smith F A and Smith S E 1996 Mutualism and Parasitism: biodiversity in function and structure in the 'arbuscular' (VA) mycorrhizal symbiosis. Adv. Bot. Res. 22, 1–43.CrossRefGoogle Scholar
  86. Smith S E and Read D J (eds) 1997 Mycorrhizal Symbiosis (2nd Edn). Academic Press: London, UK. 605 p.Google Scholar
  87. Staddon P L 1998 Insights into mycorrhizal colonisation at elevated CO2: a simple carbon partitioning model. Plant Soil 205, 171–180.CrossRefGoogle Scholar
  88. Stutz J C and Morton J B 1996 Successive pot cultures reveal high species richness of arbuscular endomycorrhizal fungi in arid ecosystems. Can. J. Bot. 74, 1883–1889.Google Scholar
  89. Thomson B D, Robson A D and Abbott K 1990 Mycorrhizas formed by Gigaspora calospora and Glomus fasciculatum on subterranean clover in relation to carbohydrate concentration in roots. New Phytol. 114, 217–255.CrossRefGoogle Scholar
  90. Tisdall JM1991 Fungal hyphae and structural stability of soil. Aust. J. Soil Res. 29, 729–743.CrossRefGoogle Scholar
  91. Tisdall J M and Oades J M 1979 Organic matter and water-stable aggregates in soils. J Soil Sci. 33, 141–163.Google Scholar
  92. Tisserant B, Brenac V, Requena N, Jeffries P and Dodd J C 1998 The detection of Glomus spp. (arbuscular mycorrhizal fungi) forming mycorrhizas in three plants, at different stages of seedling development, using mycorrhiza-specific isozymes. New Phytol. 138, 225–239.CrossRefGoogle Scholar
  93. Van der Heijden M G A, Boller T, Wiemken A and Sanders I R 1998a Different arbuscular mycorrhizal fungal species are potential determinants of plant community structure. Ecology 79, 2082–2091.CrossRefGoogle Scholar
  94. Van der Heijden M G A, Klironimos J N, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A and Sanders I R 1998b Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396, 69–72.CrossRefGoogle Scholar
  95. Van Tuinen D, Jacquot E, Zhao B, Gollotte A and Gianinazzi-Pearson V 1998 Characterisation of root colonisation profiles by a microcosm community of arbuscular mycorrhizal fungi using 25 S rDNA-targeted nested PCR. Molecular Ecology 7, 879–887.PubMedCrossRefGoogle Scholar
  96. Vierheilig H, Coughlan A P, Wyss U and Piche Y 1998 Ink and Vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Appl. Env. Microbiol. 64, 5004–5007.Google Scholar
  97. Vosatka M and Dodd, J C 1998 The role of different arbuscular mycorrhizal fungi growth of Calamagrostis villosa and Deschampsia flexuosa in experiments with simulated acid rain. Plant Soil, 200, 251–263.CrossRefGoogle Scholar
  98. Weickel B 1998 Studies on the isolation, identification and biological characterisation of arbuscular mycorrhizal fungi. Dr. Agr. Thesis Rheinischen Friedrich-Wilhelms-Universitat, Bonn, Germany.Google Scholar
  99. Widden P 1996 The morphology of vesicular-arbuscular mycorrhizae in Clintona borealis and Medeola virginiana. Can. J. Bot. 74, 679–685.CrossRefGoogle Scholar
  100. Wilson G W T and Hartnett D C 1997 Effects of mycorrhizae on plant growth and dynamics in experimental tallgrass prairie micrcosms. Am. J. Bot. 84, 478–482.CrossRefGoogle Scholar
  101. Wright S F and Upadhyaya A 1996 Extraction of an abundant and unusual protein from soil and comparison with hyphal protein from arbuscular mycorrhizal fungi. Soil Sci. 161, 575–586.CrossRefGoogle Scholar
  102. Wright S F and Upadhyaya A 1998 A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant Soil 198, 97–107.CrossRefGoogle Scholar
  103. Wright S F, Upadhyaya A and Buyer J S 1998 Comparison of Nlinked oligosaccharides of glomalin from arbuscular mycorrhizal fungi and soils by capillary electrophoresis. Soil Biol. Biochem. 13, 1853–1857.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • John C. Dodd
    • 1
  • Claire L. Boddington
    • 2
  • Alia Rodriguez
    • 2
  • Carmen Gonzalez-Chavez
    • 2
  • Irdika Mansur
    • 2
  1. 1.International Institute of Biotechnology – Biotechnology MIRCEN/Department of BiosciencesUniversity of Kent CampusCanterbury, KentUK
  2. 2.International Institute of Biotechnology – Biotechnology MIRCEN/Department of BiosciencesUniversity of Kent CampusCanterbury, KentUK

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