, Volume 16, Issue 3–4, pp 361–365 | Cite as

Pollen viability as a bio-indicator of air quality

  • Orietta Iannotti
  • Gianfranco Mincigrucci
  • Emma Bricchi
  • Giuseppe FrenguelliEmail author


Many air pollutants cause plant deterioration. In this study pollen viability was used as bio-indicator of air quality. The study was carried out in the city of Perugia where road traffic is the most important cause of air pollution.

Three areas, with different intensity of road traffic (very high, medium and absent) but all characterized by the presence of the same plant species, were selected.

Eight species were studied: Hedera helix L., Convolvulus sepium L., Cynodon dactylon (L.) Pers., Quercus ilex L., Dactylis glomerata L., Parietaria diffusa M. et K., Daucus carota L. and Tilia cordata Miller. The pollen of these species was treated with TTC (2, 3, 5 Tryphenil-Tetrazolium-Chloride) staining solution and viability was then estimated by light microscopy. The results showed that the pollen viability was inversely proportioned with pollution. The highest difference in pollen viability between the areas was registered in Tilia cordata. Quercus ilex instead showed that there was no difference in pollen viability between the three different areas. Parietaria diffusa showed a particular behaviour; the highest pollen viability percentage was in polluted areas.

The statistical analysis (ANOVA) showed that the main source of variability of the pollen viability depends on the plant but also the site and the interaction between plant and site were very important with a high significant level (p < 0.0001).

air quality bio-indicator Perugia pollen viability 


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  1. Alexander M.P.: 1969, Differential staining of aborted and nonaborted pollen. Stain Technology 44(3), 117–122.Google Scholar
  2. Bellani L.M., Paoletti E. and Cenni E.: 1988, Air pollution effects on pollen germination of Forest species. In: Cresti (ed), Sexual Reproduction in Higher Plants, pp. 265–270.Google Scholar
  3. Bricchi E., Mincigrucci G., Frenguelli G., Iannotti O., Cingolani L., Di Brizio M. and Morosi A.: 1998, Pollen: Bio-indicator of Air Quality. Abs. of 6th International Congress of Aerobiology, Perugia (Italy).Google Scholar
  4. Comtois P. and Schemenauer R.S.: 1991, Tree pollen viability in areas subject to high pollutant deposition. Aerobiologia 7, 144–151.CrossRefGoogle Scholar
  5. Comtois P.: 1994, Airborne pollen dispersal and survival on Mount Sutton (Canada). Aerobiologia 10, 31–37.CrossRefGoogle Scholar
  6. Franchi G.G., Pacini E. and Rottoli P.: 1984, Pollen grain viability in Parietaria judaica L. during the long blooming period and correlation with meteorological conditions and allergic diseases. Giorn. Bot. Ital. 118, 163–178.Google Scholar
  7. Melati M.R., Alaimo M.G., Bruno A., Palmeri E. and Todaro C.: 1997, Riscontro di piombo in piante erbacee con piccoli pollini (Parietaria). Not. Allergol. 16, 91–96.Google Scholar
  8. Sarvella P.: 1964, Vital-stain testing of pollen viability in cotton. Journal of Heredity 55, 154–158.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Orietta Iannotti
    • 1
  • Gianfranco Mincigrucci
    • 2
  • Emma Bricchi
    • 2
  • Giuseppe Frenguelli
    • 3
    Email author
  1. 1.Department of Plant BiologyUniversity of PerugiaPerugiaItaly
  2. 2.Department of Plant BiologyUniversity of PerugiaItaly
  3. 3.Department of Plant BiologyUniversity of PerugiaItaly (Author for correspondence, e-mail:

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