Molecular Breeding

, Volume 6, Issue 5, pp 441–449

Mapping candidate genes in Eucalyptus with emphasis on lignification genes

  • Jean-Marc Gion
  • Philippe Rech
  • Jacqueline Grima-Pettenati
  • Daniel Verhaegen
  • Christophe Plomion
Article
  • 138 Downloads

Abstract

We used the single-strand conformation polymorphism (SSCP) technique to map eight genes on Eucalyptus urophylla and Eucalyptus grandis linkage maps. These included four genes involved in the common phenylpropanoid pathway (caffeic acid 3-0-methyltransferase, caffeoyl CoA 3-O-methyltransferase, 4-coumarate CoA ligase and phenylalanine ammonia-lyase), two genes involved in the `lignin specific' pathway (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase), and two symbiosis regulated genes (EgHypar and EgTubA1). A novel source of variation which affects the SSCP pattern, i.e. the presence or absence of electrophoresis buffer upon loading the samples into the polyacrylamide gel, was found. The placement of these genes on the Eucalyptus maps was carried out using an interspecific hybrid mapping population. This will further facilitate the identification or exclusion of `positional' candidate genes for characterizing quantitative trait loci (QTL) for wood quality and vegetative propagation related traits.

candidate gene Eucalyptus lignins mapping single-strand conformation polymorphism 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Jean-Marc Gion
    • 1
  • Philippe Rech
    • 1
    • 2
  • Jacqueline Grima-Pettenati
    • 2
  • Daniel Verhaegen
    • 1
  • Christophe Plomion
    • 3
  1. 1.Programme Arbres et PlantationsCIRAD-ForêtMontpellierFrance
  2. 2.Pôle de Biotechnologie VégétaleUMR CNRS/Université Paul Sabatier no. 5546Castanet TolosanFrance
  3. 3.Laboratoire de Génétique et Amélioration des Arbres ForestiersINRACestasFrance

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