Hydrobiologia

, Volume 437, Issue 1–3, pp 133–137 | Cite as

A routine method of DNA-extraction from extremely small metazoans, e.g. single rotifer specimens for RAPD-PCR analyses

  • Christine Leutbecher
Article

Abstract

A simple and efficient method has been developed for isolating genomic DNA from single specimens of extremely small metazoan animals, e.g. rotifers (Rotifera: Monogononta). The amount and quality of the extracted DNA allows the Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) to be employed with up to three random primers per specimen. Reproducibility of RAPD-fingerprints has been checked and verified. Exemplarily, RAPD patterns of three Brachionus species (B. angularis, B. calyciflorus, B. urceolaris) amplified with the primer OPA 07 are demonstrated.

DNA-isolation single specimen analysis RAPD-PCR rotifer 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Christine Leutbecher
    • 1
  1. 1.Spezielle ZoologieUniversity of OsnabrückOsnabrückGermany

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