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Plant Molecular Biology

, Volume 44, Issue 5, pp 649–657 | Cite as

Circadian and senescence-enhanced expression of a tobacco cysteine protease gene

  • Tadamasa Ueda
  • Shigemi Seo
  • Yuko Ohashi
  • Junji Hashimoto
Article

Abstract

A cDNA clone encoding a cysteine protease was isolated from a tobacco cDNA library, utilizing as a probe a PCR fragment obtained from degenerated primers based on the conserved sequences of plant cysteine protease genes. A putative protein encoded by the clone NTCP-23 had an amino acid sequence with significant similarities to those of plant senescence-associated cysteine proteases and mammalian cathepsin H. Northern blot analysis showed that NTCP-23 mRNA is expressed in all organs and the mRNA and protein expression is enhanced during natural senescence. We propose that NTCP-23 is responsible for amino acid remobilization especially in senescencing leaves. Furthermore, it was found that the mRNA expression follows a circadian rhythm and is reduced by continuous darkness, wounding and hypersensitive reaction (HR). NTCP-23 is the first cysteine protease whose mRNA expression has been shown to be temporarily reduced by wounding.

circadian rhythm cysteine protease (cysteine endopeptidases EC 3.4.22) senescence tobacco (Nicotiana tabacumwounding 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Tadamasa Ueda
    • 1
    • 2
  • Shigemi Seo
    • 1
    • 2
  • Yuko Ohashi
    • 1
    • 2
  • Junji Hashimoto
    • 1
    • 2
  1. 1.National Institute of Agrobiological ResourcesIbarakiJapan
  2. 2.CRESTJapan Science and Technology Corporation, ChiyodakuTokyoJapan

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