Journal of Assisted Reproduction and Genetics

, Volume 18, Issue 2, pp 110–113

PHYSIOLOGY: The Expression of Pseudogene Cyclin D2 mRNA in the Human Ovary May Be a Novel Marker for Decreased Ovarian Function Associated with the Aging Process

  • Dooseok Choi
  • Sarah Yoon
  • Eunyoung Lee
  • Seongsoo Hwang
  • Byungkoo Yoon
  • Jeho Lee
Article

DOI: 10.1023/A:1026538826392

Cite this article as:
Choi, D., Yoon, S., Lee, E. et al. J Assist Reprod Genet (2001) 18: 110. doi:10.1023/A:1026538826392

Abstract

Purpose: Our purpose was to investigate the expression pattern of cyclin D2 and pseudogene cyclin D2 mRNA in the human ovary with age.

Methods: After extraction of the total RNA from ovarian tissues of 23 premenopausal patients, cyclin D2 and pseudogene cyclin D2 mRNAs were measured by the reverse transcription–polymerase chain reaction technique using cyclin D2 and pseudogene cyclin D2 specific primers. Analysis of the cyclin D2 and pseudogene cyclin D2 mRNA expression pattern with age and correlation analysis were carried out.

Results: A 489-bp cyclin D2 band and a 441-bp pseudogene cycin D2 mRNA band were detected in the human ovarian tissue. While cyclin D2 mRNA expression showed a decreasing tendency with age (P = 0.17), pseudogene cyclin D2 mRNA expression increased with age (P < 0.05). Pseudogene cyclin D2 mRNA expression showed a negative correlation with cyclin D2 mRNA (R = −0.35, P < 0.03).

Conclusion: The expression of pseudogene cyclin D2 mRNA in the human ovary increases with age, which may be a novel marker for decreased ovarian function associated with the aging process.

Human ovary cyclin D2 pseudogene cyclin D2 

Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Dooseok Choi
    • 1
  • Sarah Yoon
    • 1
  • Eunyoung Lee
    • 1
  • Seongsoo Hwang
    • 1
  • Byungkoo Yoon
    • 1
  • Jeho Lee
    • 1
  1. 1.Department of Obstetrics and Gynecology, Samsung Medical CenterSungkyunkwan University School of MedicineKangnam-ku, SeoulKorea

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