Biologia Plantarum

, Volume 43, Issue 1, pp 153–155 | Cite as

Primer Design and Optimization for RAPD Analysis of Nepenthes

  • S.H. Lim
  • D.C.Y. Phua
  • H.T.W. Tan
Article

Abstract

Primers with higher G+C content produced better random amplified polymorphic DNA (RAPD) profiles in Nepenthes. The occurrence of clustered G's and C's in the center of the primer seemed also to influence the banding patterns. It was also observed that for certain polymerases, the use of different buffers other than that recommended by the manufacturer provided a better amplification profile for Nepenthes.

GC content PCR buffer RAPD markers 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • S.H. Lim
    • 1
  • D.C.Y. Phua
    • 1
  • H.T.W. Tan
    • 1
  1. 1.Department of Biological SciencesThe National University of SingaporeSingaporeRepublic of Singapore e-mail

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