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Antonie van Leeuwenhoek

, Volume 78, Issue 2, pp 107–115 | Cite as

Actinoplanes capillaceus sp. nov., a new species of the genus Actinoplanes

  • Atsuko Matsumoto
  • Yoko Takahashi
  • Takuji Kudo
  • Akio Seino
  • Yuzuru Iwai
  • Satoshi Omura
Article

Abstract

Two motile actinomycete strains, K95–5561T and K95–5562, were isolated from a soil sample collected at Sayama City, Saitama Prefecture, Japan. They produced bell shaped spore vesicles (sporangia) with hairy surfaces on substrate hyphae. When released into water, the sporangiospores became motile by a tuft of polar flagella. The chemotaxonomic and morphological characteristics together with 16S rRNA gene sequence data indicated that the two isolates belonged to the genus Actinoplanes. The two strains were assigned to a single species on the basis of phenotypic, notably cultural, morphological and physiological characteristics, and DNA-DNA pairing data. The two strains were distinguished from representatives of all validly described species of Actinoplanes using a combination of genotypic and phenotypic properties. It is, therefore, proposed that strains K95–5561 and K95–5562 be recognized as a new species of the genus Actinoplanes with the name Actinoplanes capillaceus sp. nov. The type strain of the species is strain K95–5561T (=JCM 10268T =IFO 16408T). The invalidly proposed species `Ampullariella cylindrica', `Ampullariella pekinensis' and `Ampullariella pilifera' were assigned to Actinoplanes capillaceus on the basis of genotypic and phenotypic data.

Actinoplanes capillaceus sp. nov. hairy sporangium motile actinomycete polyphasic taxonomy 

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References

  1. Becker B, Lechevalier MP & Lechevalier HA (1965) Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl. Microbiol. 13: 236–243PubMedGoogle Scholar
  2. Brosius J, Palmer ML, Kennedy PJ & Noller HF (1978) Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75: 4801–4805PubMedGoogle Scholar
  3. Collins MD, Goodfellow M & Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J. Gen. Microbiol. 100: 221–230PubMedGoogle Scholar
  4. Couch JN (1950) Actinoplanes, a new genus of the Actinomycetales. J. Elisha Mitchell Sci. Soc. 66: 87–92Google Scholar
  5. Couch JN (1963) Some new genera and species of the Actinoplanaceae. J. Elisha Mitchell Sci. Soc. 79: 53–70Google Scholar
  6. Couch JN (1964) A proposal to replace the name Ampullaria Couch with Ampullariella. J. Elisha Mitchell Sci. Soc. 89: 29Google Scholar
  7. Euzéby JP (1997) List of bacterial names with standing in nomenclature: a folder available on the Internet. Int. J. Syst. Bacterol. 47: 590–592Google Scholar
  8. Ezaki T, Hashimoto Y & Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39: 224–229Google Scholar
  9. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791Google Scholar
  10. Gordon RE, Barnett DA, Handerhan JE & Pang CH (1974) Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int. J. Syst. Bacteriol. 24: 54–63Google Scholar
  11. Hasegawa T, Takizawa M & Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J. Gen. Appl. Microbiol. 29: 319–322Google Scholar
  12. Hayakawa M, Tamura T & Nonomura H (1991) Selective isolation of Actinoplanes and Dactylosporangium from soil by using ?-collidine as the chemoattractant. J. Ferment. Bioeng. 72: 426–432Google Scholar
  13. Jiang C & Ruan J (1982) Two new species and a new variety of Ampullariella. Acta Microbiol. Sinica 22: 207–211Google Scholar
  14. Jiang C, Xu L & Ruan J (1983) New species of Actinoplanes and Ampullariella. Acta Microbiol. Sinica 23: 210–215Google Scholar
  15. Juan C & Zhang Y (1974) A taxonomic study of Actinoplanaceae. 1. Classification of Ampullariella. Acta Microbiol. Sinica 14: 31–41Google Scholar
  16. Lechevalier MP, De Biévre C & Lechevalier HA (1977) Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem. Syst. Ecol. 5: 249–260Google Scholar
  17. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3: 208–218Google Scholar
  18. Minnikin DE, Patel PV, Alshamaony L & Goodfellow M (1977) Polar lipid composition in the classification of Nocardia and related bacteria. Int. J. Syst. Bacteriol. 27: 104–117Google Scholar
  19. Pridham TG & Gottlieb D (1948) The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J. Bacteriol. 56: 107–114Google Scholar
  20. Saitou N & Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425PubMedGoogle Scholar
  21. Shirling EB & Gottlieb D (1966) Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16: 313–340Google Scholar
  22. Stackebrandt E & Kroppenstedt M (1987) Union of genera Actinoplanes Couch, Ampullariella Couch, and Amorphosporangium Couch in a redefined genus Actinoplanes. Syst. Appl. Microbiol. 9: 110–114Google Scholar
  23. Suzuki K & Komagata K (1983) Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int. J. Syst. Bacteriol. 33: 188–200Google Scholar
  24. Tamaoka J, Katayama-Fujimura Y & Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J. Appl. Bacteriol. 54: 31–36Google Scholar
  25. Tamaoka J & Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25: 125–128Google Scholar
  26. Taylor HD, Knoche L & Granville WC (Eds.) (1958) Color Harmony Manual 4th Edn. Container Corporation of America, ChicagoGoogle Scholar
  27. Thompson JD, Higgins DG & Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence aligmnent through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673–4680PubMedGoogle Scholar
  28. Tomiyasu I (1982) Mycolic acid composition and thermally adaptative changes in Nocardia asteroides. J. Bacteriol. 151: 828–837PubMedGoogle Scholar
  29. Uchida K & Aida K (1977) Acyl type of bacterial cell wall: Its simple identification by colorimetric method. J. Gen. Appl. Microbiol. 23: 249–260Google Scholar
  30. Waksman SA (1961) The actinomycetes. Vol. 2, Classification, identification and description of genera and species. Williams and Wilkins Co., BaltimoreGoogle Scholar
  31. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP & Trüper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37: 463–464Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Atsuko Matsumoto
    • 1
  • Yoko Takahashi
    • 1
  • Takuji Kudo
    • 2
  • Akio Seino
    • 1
  • Yuzuru Iwai
    • 1
  • Satoshi Omura
    • 3
  1. 1.Research Center for Biological FunctionThe Kitasato InstituteTokyoJapan
  2. 2.Japan Collection of MicroorganismsRIKEN (The Institute of Physical and Chemical Research)SaitamaJapan
  3. 3.Research Center for Biological FunctionThe Kitasato InstituteTokyoJapan

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