Pharmaceutical Research

, Volume 17, Issue 10, pp 1159–1167 | Cite as

Protein Instability in Poly(Lactic-co-Glycolic Acid) Microparticles

  • Marco van de Weert
  • Wim E. Hennink
  • Wim Jiskoot


In this review the current knowledge of protein degradation during preparation, storage and release from poly(lactic-co-glycolic acid) (PLGA) microparticles is described, as well as stabilization approaches. Although we have focussed on PLGA microparticles, the degradation processes and mechanisms described here are valid for many other polymeric release systems. Optimized process conditions as well as stabilizing excipients need to be used to counteract several stress factors that compromise the integrity of protein structure during preparation, storage, and release. The use of various stabilization approaches has rendered some success in increasing protein stability, but, still, full preservation of the native protein structure remains a major challenge in the formulation of protein-loaded PLGA microparticles.

controlled release microparticles PLGA protein stability stabilization 


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Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Marco van de Weert
    • 1
  • Wim E. Hennink
    • 1
  • Wim Jiskoot
    • 1
    • 2
  1. 1.Department of Pharmaceutics, Utrecht Institute for Pharmaceutical SciencesUtrecht UniversityUtrechtThe Netherlands
  2. 2.Department of Pharmaceutics, Utrecht Institute for Pharmaceutical SciencesUtrecht UniversityUtrechtThe Netherlands

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