Journal of Assisted Reproduction and Genetics

, Volume 17, Issue 10, pp 574–579

Clinical Correlation of Cyclin D2 mRNA Expression in Human Luteinized Granulosa Cells

  • Dooseok Choi
  • Eun Young Lee
  • Sarah Yoon
  • Seongsoo Hwang
  • Byung-Koo Yoon
  • Je-Ho Lee
Article

DOI: 10.1023/A:1026470825514

Cite this article as:
Choi, D., Lee, E.Y., Yoon, S. et al. J Assist Reprod Genet (2000) 17: 574. doi:10.1023/A:1026470825514

Abstract

Purpose: The aim was to evaluate whether the expression of cyclin D2 mRNA in human luteinized granulosa cells correlates with the clinical parameters of women undergoing in vitro fertilization–embryo transfer (IVF-ET) programs.

Methods: Using reverse transcriptase–polymerase chain reaction (RT-PCR) techniques, the cyclin D2 mRNA in human luteinized granulosa cells of large follicles was measured. Correlation analysis between cyclin D2 mRNA expression and clinical parameters [age; day3 follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2); E2 and P4 at the day of human chorionic gonadotropin (hCG); total amount of gonadotropin and the number of days of gonadotropin administration; pregnancy outcome] were performed.

Result(s): Cyclin D2 mRNA expression showed negative correlation with patient's age (R = −0.60, P < 0.005) and total amount of gonadotropins (R = −0.45, P < 0.03), and also showed positive correlation with serum E2 levels at the day of hCG administration (R = 0.55, P < 0.02). Other clinical parameters showed no statistical significance. Cyclin D2 mRNA expression of pregnant group was higher than that of nonpregnant group (P < 0.03).

Conclusions: Cyclin D2 mRNA expression in human luteinized granulosa cells positively correlates with clinical parameters of ovarian function and may be a novel marker for the health of human ovarian follicle.

cyclin D2 mRNA human luteinized granulosa cell clinical parameters 

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Dooseok Choi
    • 1
  • Eun Young Lee
    • 2
  • Sarah Yoon
    • 3
  • Seongsoo Hwang
    • 2
  • Byung-Koo Yoon
    • 1
  • Je-Ho Lee
    • 1
  1. 1.Department of Obstetrics and Gynecology, Samsung Medical CenterSungkyunkwan University School of MedicineSeoulKorea
  2. 2.IVF ClinicSamsung Medical CenterSeoulKorea
  3. 3.Clinical Research CenterSamsung Biomedical Research InstituteSeoulKorea

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