Glycoconjugate Journal

, Volume 16, Issue 2, pp 147–159

Enzymatic synthesis of nucleotide sugars

  • Thomas Bülter
  • Lothar Elling
Article
  • 476 Downloads

Abstract

The present review gives a survey on the biosynthetic pathways of nucleotide sugars which are important for the in vitro synthesis of mammalian glycoconjugates. With respect to the use of these enzymes in glycotechnology the availability as recombinant enzymes from different sources, the large-scale synthesis of nucleotide sugars and their in situ regeneration in combination with glycosyltransferases are summarized and evaluated.

enzymatic synthesis nucleotide sugars ADP Adenosine 5′9-diphosphate CDP Cytidine 5′9-diphosphate CMP Cytidine 5′9-monophosphate CMP-Neu5Ac Cytidine 5′9-monophospho-N-acetyl-β-D-neuraminic acid CTP Cytidine 5′9-triphosphate dTDP 2′9-deoxythymidine 5′9-diphosphate dUDP 2′9-deoxyuridine 5′9-diphosphate EMR enzyme membrane reactor Fru D-fructose Fru-6-P D-fructose 6-phosphate Fuc L-fucose Fuc-1-P β-L-fucose 1-phosphate Gal D-galactose Gal-1-P α-D-galactose 1-phosphate GalN D-galactosamine GalN-1-P α-D-galactosamine 1-phosphate GalNAc N-acetyl-D-galactosamine GalNAc-1-P N-acetyl-α-D-galactosamine 1-phosphate GDP Guanosine 5′9-diphosphate GDP-6-d-4-k-L-Gal Guanosine 5′9-diphospho-6-deoxy-4-keto-β-L-galactose GDP-6-d-4-k-Man Guanosine 5′9-diphospho-6-deoxy-4-keto-α-D-mannose GDP-Fuc Guanosine 5′9-diphospho-β-L-fucose GDP-Fuc PP Guanosine 5′9-diphospho-β-L-fucose pyrophosphorylase GDP-Man Guanosine 5′9-diphospho-α-D-mannose GDP-Man DHy GDP-mannose-4,6-dehydratase (EC 4.2.1.47) GDP-Man PP GDP-man pyrophosphorylase (EC 2.7.7.13) Glc-1-P D-glucose 1-phosphate Glc-6-P D-glucose 6-phosphate GlcA-1-P α-D-glucuronic acid 1-phosphate GlcN D-glucosamine GlcN-1-P α-D-glucosamine 1-phosphate GlcN-6-P D-glucosamine 6-phosphate GlcNAc N-acetyl-D-glucosamine GlcNAc-1-P N-acetyl-α-D-glucosamine 1-phosphate GlcNAc-6-P N-acetyl-D-glucosamine 6-phosphate GlmU glucosamine uridyltransferase GTP Guanosine 5′9-triphosphate LacNAc N-acetyllactosamine Lex Lewis X antigen Man D-mannose Man-1-P α-D-mannose 1-phosphate Man-6-P D-mannose 6-phosphate ManNAc N-acetyl-D-mannose ManNAc-6-P N-acetyl-D-mannose 6-phosphate NAD1 nicotinamide adenine dinucleotide NADH nicotinamide adenine dinucleotide hydride NADP1 nicotinamide adenine dinucleotide phosphate NADPH nicotinamide adenine dinucleotide phosphate hydride NAS N-acetoxysuccinimide Neu5Ac N-acetylneuraminic acid Neu5Ac-9-P N-acetylneuraminic acid 9-phosphate NeuAc aldolase N-acetylneuraminic acid aldolase (EC 4.1.3.3) NMPK nucleoside 5′9-monophosphate kinase (EC 2.7.7.4) PEP phosphoenolpyruvate PP pyrophosphorylase Pi inorganic phosphate PPi inorganic pyrophosphate PGM phosphoglucomutase (EC 2.7.5.1) PK pyruvate kinase (EC 2.7.1.40) PMI phosphomannose isomerase (EC 5.3.1.8) PMM phosphomannomutase (EC 5.4.2.8) Pyr pyruvate UDP uridine 5′9-diphosphate UDP-Gal uridine 5′9-diphospho-α-D-galactose UDP-GalNAc uridine 5′9-diphospho-N-acetyl-α-D-galactosamine UDP-GalN uridine 5′9-diphospho-α-D-galactosamine UDP-Glc uridine 5′9-diphospho-α-D-glucose UDP-Glc DH UDP-glucose dehydrogenase UDP-GlcA uridine 5′9-diphospho-α-D-glucuronic acid UDP-GlcNAc uridine 5′9-diphospho-N-acetyl-α-D-glucosamine UDP-Xyl uridine 5′9-diphospho-α-D-xylose UMP uridine 5′9-monophosphate 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Thomas Bülter
    • 1
  • Lothar Elling
    • 1
  1. 1.Institute of Enzyme TechnologyUniversity of Düsseldorf, Research Center JülichJülichGermany

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