Journal of Mammary Gland Biology and Neoplasia

, Volume 2, Issue 4, pp 355–364 | Cite as

Mammary Gland Development in Prolactin Receptor Knockout Mice

  • Christopher J. Ormandy
  • Nadine Binart
  • Paul A. Kelly
Article

Abstract

A germ line null mutation of the prolactin receptor gene has been produced by replacing exon 5 with the Tk-NEO3 cassette. Heterozygous females showed almost complete failure of lactation following their first pregnancy when mated at 6–8 weeks of age. The severity of this phenotype was reduced when heterozygotes were mated at 20 weeks and was absent following a second pregnancy when assessed by pup survival, although a longer lag time prior to weight increase at normal rates was seen in these litters. Histological and whole mount analysis of virgin mammary glands showed that heterozygous glands were smaller in size due to a less developed ductal structure with fewer branch points. Virgin knockout glands were small, composed of sparse, large, poorly branched ducts. This work provides an ideal model to further study the role of the prolactin receptor and its ligands in mammary development and physiology.

Prolactin prolactin receptor mammary gland development knockout 

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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Christopher J. Ormandy
    • 1
    • 2
  • Nadine Binart
    • 1
    • 2
  • Paul A. Kelly
    • 1
    • 2
  1. 1.Cancer Research ProgramGarvan Institute of Medical ResearchDarlinghurstAustralia
  2. 2.INSERM UnitéParisFrance

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