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Journal of Protein Chemistry

, Volume 16, Issue 5, pp 421–423 | Cite as

Combination of Micropreparative Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for Peptide Analysis

  • Ann-Charlotte Bergman
  • Tomas Bergman
Article

Abstract

Signal suppression is a problem in matrix-assisted laser desorption/ionization mass spectrometry of peptides prepared by capillary electrophoresis. Many common electrolytes that are efficient for separation, such as sodium phosphate, also are strongly suppressive during laser desorption/ionization. We have tested individual electrolytes for highest performance in each step of separation and collection, respectively. Suppression is not observed if citrate, trifluoroacetic acid, or hydrochloric acid is used for collection, while phosphate still can be employed in the capillary providing excellent resolution. Low concentrations of hydrochloric acid added to the sample/matrix mixture generate mass spectra with better ion intensities than if trifluoroacetic acid or citrate is used.

Capillary electrophoresis peptides micropreparation matrix-assisted laser desorption/ionization mass spectrometry 

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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Ann-Charlotte Bergman
    • 1
  • Tomas Bergman
    • 1
  1. 1.Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSweden

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