Biotechnology Letters

, Volume 25, Issue 21, pp 1827–1831 | Cite as

Bioconversion of compactin into pravastatin by Streptomyces sp.

  • Joo-Woong Park
  • Joo-Kyung Lee
  • Tae-Jong Kwon
  • Dong-Hee Yi
  • Young-Jun Kim
  • Seong-Hoon Moon
  • Hyun-Hyo Suh
  • Sang-Mo Kang
  • Yong-Il Park

Abstract

Streptomyces sp. Y-110, isolated from soil, modified compactin to pravastatin, a therapeutic agent for hypercholesterolemia. In a batch culture, the highest production of pravastatin was 340 mg l−1 from 750 mg compactin l−1 in 24 h. By intermittent feeding of compactin into the culture medium, both the compactin concentration and its conversion increased to 2000 mg l−1 and 1000 mg pravastatin l−1, respectively, with the conversion rate of 10 mg l−1 h−1. Continuous feeding of compactin increased production of pravastatin to 15 mg l−1 h−1.

bioconversion compactin cytochrome P-450 pavastatin Streptomyces sp. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Haruyuki Y, Tsubokawa S, Endo A (1985) Microbial hydroxylation of compactin (ML-236B) and monacolin K. J. Antibiotics 38: 605–609.Google Scholar
  2. Holt JG (1994) Bergey's Manual of Determinative Bacteriology, 9th edn. Baltmore: Williams and Wilkins Co.Google Scholar
  3. Masahiko H, Ogawa K, Yoshikawa H (1993) Application of computer to monitoring and control of fermentation process: microbial conversion of ML-236B Na to pravastatin. Biotechnol. Bioeng. 42: 815–820.CrossRefGoogle Scholar
  4. Omura T, Sato R (1964) The carbon monooxide-binding pigment of liver microsomes. J. Biol. Chem. 239: 2370–2378.PubMedGoogle Scholar
  5. Park JW, Lee JK, Kwon TJ, YI DH, Park YI, Kang SM (2001) Purification and characterization of a cytochrome P-450 from pravatstatin-producing Streptomyses sp. Y-110. J. Microbiol. Biotechnol. 11: 1011–1017.Google Scholar
  6. Shepherd J, Cobbe SM, Ford I, Isles CG, Lorimer AR, Macferlane PW, Mckillop JH, Packard CJ (1995) Prevention of coronary heart disease with pravastatin in men with hypercholesterolemia. New Engl. J. Med. 333: 1301–1309.PubMedGoogle Scholar
  7. Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16: 313–340.Google Scholar
  8. Willcox WB, Vapage SP, Bascomb S, Curtis MA (1973) Identification of bacteria by computer: theory and programming. J. Gen. Microbiol. 77: 317–310.PubMedGoogle Scholar
  9. Williams ST, Goodfellow M, Alderson G, Wellington EMH, Sneath PHA, Sackin M (1983) Numerical classification of Streptomyces and related genera. J. Gen. Microbiol. 129: 1743–1813.PubMedGoogle Scholar
  10. Williams ST, Sharpe ME, Holt JG, Murray RG, Brener DJ, Krieg NR, Mouldar JW, Pfenning N, Sneath PHA, Staley JT (1989) Bergey's Mannual of Systematic Bacteriology, Vol.4. Baltimore: William & Willkins Co.Google Scholar
  11. Yulin P, Yashphe J, Demain AL (1997) Biotransformation of compactin to pravastatin by Actinomadura sp. 2966. J. Antibiotics 50: 1032–1035.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Joo-Woong Park
    • 1
  • Joo-Kyung Lee
    • 1
  • Tae-Jong Kwon
    • 2
  • Dong-Hee Yi
    • 2
  • Young-Jun Kim
    • 3
  • Seong-Hoon Moon
    • 3
  • Hyun-Hyo Suh
    • 4
  • Sang-Mo Kang
    • 2
  • Yong-Il Park
    • 3
  1. 1.Biotechnology LaboratoryYoungjin Pharmaceutical Co. Ltd.Pyungtaek-SiKorea
  2. 2.Department of Microbial EngineeringKon Kuk UniversitySeoulKorea
  3. 3.Division of Biotechnology and The Collaborative Institute of Science and TechnologyThe Catholic University of KoreaWonmi-gu, Bucheon City, Gyonggi-doKorea
  4. 4.Department of Environmental EngineeringChinju National UniversityChinjuKorea

Personalised recommendations