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Biotechnology Letters

, Volume 25, Issue 21, pp 1833–1835 | Cite as

Fed-batch fermentation of Lactobacillus lactis for hyper-production of l-lactic acid

  • Dong-Mei Bai
  • Qiang Wei
  • Zhi-Hui Yan
  • Xue-Ming ZhaoEmail author
  • Xin-Gang Li
  • Shi-Min Xu
Article

Abstract

A fed-batch fermentation of Lactobacillus lactis to produce l-lactic acid was developed in which the residual glucose concentration in the culture was used to control a continuous feeding strategy. Up to 210 g l-lactic acid l−1 (97% yield) was obtained. The maximal dry cell was 2.7 g l−1 and the average l-lactic acid productivity was 2.2 g l−1 h−1.

fed-batch fermentation glucose feeding Lactobacillus lactis l-lactic acid 

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References

  1. Bai DM, Jia MZ, Zhao XM, Ban R, Shen F, Li XG, Xu SM (2003) L(+)-Lactic acid production by pellet form Rhizopus oryzae R1021 in a stirred tank fermenter. Chem. Eng. Sci. 58: 785–791.CrossRefGoogle Scholar
  2. Chen R, Lee YY (1997) Membrane-mediated extractive fermentation for lactic acid production from cellulosic biomass. Appl. Biochem. Biotechnol. 63–65: 435–438.Google Scholar
  3. Datta R, Tsai SP, Bonsignore P, Moon SH, Frank JR (1995) The technologyical and economic potential of poly (lactic acid) and its derivatives. FEMS Microbiol. Rev. 16: 221–231.Google Scholar
  4. Roukas T, Kotzekidou P (1998) Lactic acid production from deproteinized whey by mixed cultures of free and coimmobilized Lactobacillus casei and Lactococcus lactis cells using fedbatch culture. Enzyme Microb. Technol. 22: 199–204.Google Scholar
  5. Siebold M, von Friling P, Joppien R, Rindfleisch D, Schügerl K, Röper H (1995) Comparison of the production of lactic acid by three different lactobacilli and its recovery by extraction and electrodialysis. Proc. Biochem. 30: 81–95.Google Scholar
  6. Tsai TS, Millard CS (1994) Improved Pretreatment Process for Lactic Acid Production. PCT Int. Appl. Patent, WO 94/13826; PCT/US93/11759.Google Scholar
  7. Tyree RW, Clausen EC, Gaddy JL (1990) The fermentative characteristics of Lactobacillus xylosus on glucose and xylose. Biotechnol. Lett. 12: 51–56.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Dong-Mei Bai
    • 1
    • 2
  • Qiang Wei
    • 1
    • 2
  • Zhi-Hui Yan
    • 3
  • Xue-Ming Zhao
    • 3
    Email author
  • Xin-Gang Li
    • 2
  • Shi-Min Xu
    • 3
  1. 1.Department of Biochemical Engineering, School of Chemical Engineering & TechnologyP.R. China
  2. 2.National Engineering Research Center for Distillation TechnologyTianjin UniversityTianjinP.R. China
  3. 3.Department of Biochemical Engineering, School of Chemical Engineering & TechnologyTianjin UniversityTianjinP.R. China

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