Journal of Fluorescence

, Volume 13, Issue 5, pp 403–420

Autofluorescence of Plant Secreting Cells as a Biosensor and Bioindicator Reaction

Article

DOI: 10.1023/A:1026164922760

Cite this article as:
Roshchina, V.V. Journal of Fluorescence (2003) 13: 403. doi:10.1023/A:1026164922760

Abstract

Autofluorescence of intact secretory cell of plants is considered as a possible biosensor for the cellular biomonitoring. The fluorescence of secretory cells was due to the chemical composition of their secretions. This phenomenon could be recommended: 1. for the diagnostics of secretory cells among non-secretory ones; 2. for the express-analysis of the content of secretory cells at norm and under various factors; 3. for the analysis of cell-cell interactions. Identification of intact secretory cells filled with products of secondary metabolism is based on their fluorescence characteristics. The filling of the cells with a secretion and the removing of the secretions are easily observed under the luminescence microscope—from confocal microscope to various microspectrofluorimeters. The cellular fluorescence can serve as an indicator of the cell state in vivo. The following development of the earlier diagnostics based on the autofluorescence may be the area of the remote monitoring of agriculture crops and yields of medicinal plants as well as the remote sensing of the environmental stress.

Autofluorescence confocal microscopy luminescence microscopy microspectrofluorimetry plants secondary metabolites secretory cells spectral analysis 

Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  1. 1.Laboratory of Microspectral Analysis of Cells and Cellular SystemsInstitute of Cell Biophysics RAN, PushchinoMoscowRussia

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