European Journal of Plant Pathology

, Volume 109, Issue 8, pp 841–850 | Cite as

Eyespot of Cereals Revisited: ITS phylogeny Reveals New Species Relationships

  • Pedro W. Crous
  • J.Z. (Ewald) Groenewald
  • Walter Gams

Abstract

Four species so far classified in Pseudocercosporella or Ramulispora (hyphomycetes) are associated with eyespot disease symptoms of cereals. Two of these have been linked to teleomorphs that were described in Tapesia. Sequence data derived from the Internal Transcribed Spacer region (ITS1, 5.8S and ITS2) of the rDNA operon showed, however, that the eyespot fungi associated with Tapesia are not congeneric with Ramulispora sorghi, the type of Ramulispora. The genus name Tapesia is now rejected in favour of the conserved name Mollisia, which appears to comprise heterogeneous fungi. Tapesia yallundae is not closely related to the type of Mollisia, M. cinerea, but clusters separately, being more closely allied to species with Cadophora anamorphs. A new holomorph genus, Oculimacula, is therefore proposed for teleomorphs of the eyespot fungi, while the anamorphs are accommodated in Helgardia gen. nov.

anamorph–teleomorph relationships eyespot disease Helgardia Oculimacula phylogeny Ramulispora systematics Tapesia 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aebi B (1972) Untersuchungen über Discomycetes aus der Gruppe Tapesia-Trichobelonium. Nova Hedwigia 23: 49-112Google Scholar
  2. Arx JA von (1983) Mycosphaerella and its anamorphs. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen, Series C 86(1): 15-54Google Scholar
  3. Baral HO (1985) Bausteine zu einer Askomyzeten-Flora der BR Deutschland: In Süddeutschland gefundene Inoperculate Discomyzeten mit taxonomischen, ökologischen und chorologischen Hinweisen. Beihefte zur Zeitschrift für Mykologie 6: 1-160Google Scholar
  4. Baral HO (1994) Comments on 'Outline of the Ascomycetes-1993'. Systema Ascomycetum 13: 113-128Google Scholar
  5. Boerema GH, Pieters R and Hamers MEC (1992) Check-list for scientific names of common parasitic fungi. Supplement Series 2b (additions and corrections): Fungi on field crops: Cereals and grasses. Netherlands Journal of Plant Pathology 98(Suppl 1): 1-32Google Scholar
  6. Bouldier E (1884) Nouvelle classification naturelle des discomycètes charnus, connus généralement sous le nom de Pezizes. Bulletin of the Mycological Society of France 1: 91-120Google Scholar
  7. Braun U (1995) A Monograph of Cercosporella, Ramularia and Allied Genera (Phytopathogenic Hyphomycetes), Vol 1. IHW Verlag, Eching, GermanyGoogle Scholar
  8. Crous PW, Aptroot A, Kang JC, Braun U and Wingfield MJ (2000) The genus Mycosphaerella and its anamorphs. In: Seifert KA, Gams W, Crous PW and Samuels G (eds) Molecules, Morphology and Classification: Towards Monophyletic Genera in the Ascomycetes. Studies in Mycolology 45: 107-121Google Scholar
  9. Crous PW, Kang JC and Braun U (2001) A phylogenetic redefinition of anamorph genera in Mycosphaerella based on ITS rDNA sequence and morphology. Mycologia 93: 1081-1101Google Scholar
  10. Daniels A, Lucas JA and Peberdy JF (1991) Morphology and ultrastructure of W and R pathotypes of Pseudocercosporella herpotrichoides on wheat seedlings. Mycological Research 95: 385-397Google Scholar
  11. Deighton FC (1973) Studies on Cercospora and allied genera. IV. Cercosporella Sacc., Pseudocercosporella gen. nov. and Pseudocercosporidium gen. nov. Mycological Papers 133: 1-62Google Scholar
  12. Dennis RWG (1968) British Ascomycetes. J. Cramer, Lehre, GermanyGoogle Scholar
  13. Dyer PS, Papaikonomou M, Lucas JA and Peberdy JF (1994) Isolation of R-type progeny of Tapesia yallundae from apothecia on wheat stubble in England. Plant Pathology 43: 1039-1044Google Scholar
  14. Eriksson T (1998) AutodecayVersion 4.0. Department of Botany, Stockholm University, StockholmGoogle Scholar
  15. Fernandez FA, Glawe DA and Sinclair JB (1991) Microcycle conidiation and nuclear behavior during conidiogenesis in Cercospora kikuchii. Mycologia 83: 752-757Google Scholar
  16. Fitt BDL, Goulds A, Hollins TW and Jones DR (1990) Strategies for control of eyespot (Pseudocercosporella herpotrichoides) in UK winter wheat and winter barley. Annals of Applied Biology 117: 473-486Google Scholar
  17. Fron G (1912) Contribution à l'étude de la maladie de 'pied noir des cereales' ou 'maladie du piétin'. Annales de la Science Agronomique Française et Étrang ère, série 4, 1: 3-29Google Scholar
  18. Gams W (1992) Report of the Committee for Fungi and Lichens: New series, 2. Taxon 41: 99-108Google Scholar
  19. Gams W (2000) Phialophora and some similar morphologically little-differentiated anamorphs of divergent ascomycetes. In: Seifert KA, Gams W, Crous PW and Samuels G (eds) Molecules, Morphology and Classification: Towards Monophyletic Genera in the Ascomycetes. Studies in Mycology 45: 187-199Google Scholar
  20. Hawksworth DL and David JC (1989) Proposal to conserve Mollisia (E.M. Fries) P. Karsten over Tapesia (Pers.: E.M. Fries) Fuckel (Fungi). Taxon 38: 496Google Scholar
  21. Hillis DM and Bull JJ (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42: 182-192Google Scholar
  22. Hütter R (1958) Untersuchungen über die Gattung Pyrenopeziza Fuck. Phytopathologische Zeitschrift 33: 1-54Google Scholar
  23. Lucas JA, Dyer PS and Murray TD (2000) Pathogenicity, hostspecificity, and population biology of Tapesia spp., causal agents of eyespot disease in cereals. Advances in Botanical Research 33: 226-258Google Scholar
  24. Mchau GRA, Crous PW and Nowell DC (1996) Newly recorded foliar diseases on pearl millet (Pennisetum glaucum) and sorghum (Sorghum bicolor) in South Africa. South African Journal of Science 92(Suppl 1): xivGoogle Scholar
  25. Nauta MM and Spooner B (2000) British Dematiaceae: 4B. Demateoideae Genera G-Z. Mycologist 14: 65-74Google Scholar
  26. Nirenberg HI (1981) Differentiation of Pseudocercosporella strains causing foot rot disease of cereals. 1. Morphology. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 88: 241-248Google Scholar
  27. Olive LS, Lefebvre CL and Sherwini HS (1946) The fungus that causes sooty stripe of Sorghum spp. Phytopathology 36: 190-200Google Scholar
  28. Page RDM (1996) TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357-358Google Scholar
  29. Priestley RA, Dewey FM, Nicholson P and Rezanoor HN (1992) Comparison of isoenzyme and DNA markers for differentiating W-, R-and C-pathotypes of Pseudocercosporella herpotrichoides. Plant Pathology 41: 591-599Google Scholar
  30. Rambaut A (2002) Sequence Alignment Editor v2.0 (programme distributed by the author). Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UKGoogle Scholar
  31. Rehm H (1891) Die Pilze Deutschlands, Österreichs und der Schweiz. Rabenhorst Kryptogamenflora 1: 1-1168Google Scholar
  32. Robbertse B, Campbell GF and Crous PW (1995) Revision of Pseudocercosporella-like species causing eyespot disease of wheat. South African Journal of Botany 61: 43-48Google Scholar
  33. Robbertse B, Crous PW and Holz G (1994) Tapesia yallundae collected from wheat stubble in South Africa. Mycopathologia 125: 23-28Google Scholar
  34. Saccardo PA (1880) Sylloge Fungorum 8: 1-1143. Pudua, ItalyGoogle Scholar
  35. Scott PR and Hollins TW (1974) Effects of eyespot on the yield of winter wheat. Annals of Applied Biology 78: 269-279Google Scholar
  36. Scott PR and Hollins TW (1980) Pathogenic variation in Pseudocercosporella herpotrichoides. Annals of Applied Biology 94: 297-300Google Scholar
  37. Stewart EL, Liu Z, Crous PW and Szabó L (1999) Phylogenetic relationships among some cercosporoid anamorphs of Mycosphaerella based on rDNA sequence analysis. Mycological Research 103: 1491-1499Google Scholar
  38. Swofford DL (2000) PAUP* 4.0: Phylogenetic Analysis Using Parsimony. Sinauer Associates, Sunderland, MA, USAGoogle Scholar
  39. Thompson JD, Higgins DG and Gibson TJ (1994) Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673-4680Google Scholar
  40. Wallwork H and Spooner B (1988) Tapesia yallundae-the teleomorph of Pseudocercosporella herpotrichoides. Transactions of the British Mycological Society 91: 703-705Google Scholar
  41. Webster J, Shearer CA and Spooner BM (1993) Mollisia casaresiae (Ascomycetes) the teleomorph of Casaresia sphagnorum, an aquatic fungus. Nova Hedwigia 57: 483-487Google Scholar
  42. White TJ, Bruns T, Lee S and Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ and White TJ (eds) PCR Protocols: A Guide to Methods and Applications (pp 315-322) Academic Press, San Diego, CA, USAGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Pedro W. Crous
    • 1
  • J.Z. (Ewald) Groenewald
    • 1
  • Walter Gams
    • 1
  1. 1.Centraalbureau Voor SchimmelculturesFungal Biodiversity CentreUtrechtThe Netherlands

Personalised recommendations