Plant and Soil

, Volume 255, Issue 1, pp 19–26 | Cite as

Growth and cell wall changes in rice roots during spaceflight

  • Takayuki HosonEmail author
  • Kouichi Soga
  • Kazuyuki Wakabayashi
  • Seiichiro Kamisaka
  • Eiichi Tanimoto


We analyzed the changes in growth and cell wall properties of roots of rice (Oryza sativa L. cv. Koshihikari) grown for 68.5, 91.5, and 136 h during the Space Shuttle STS-95 mission. In space, most of rice roots elongated in a direction forming a constant mean angle of about 55° with the perpendicular base line away from the caryopsis in the early phase of growth, but later the roots grew in various directions, including away from the agar medium. In space, elongation growth of roots was stimulated. On the other hand, some of elasticity moduli and viscosity coefficients were higher in roots grown in space than on the ground, suggesting that the cell wall of space-grown roots has a lower capacity to expand than the controls. The levels of both cellulose and the matrix polysaccharides per unit length of roots decreased greatly, whereas the ratio of the high molecular mass polysaccharides in the hemicellulose fraction increased in space-grown roots. The prominent thinning of the cell wall could overwhelm the disadvantageous changes in the cell wall mechanical properties, leading to the stimulation of elongation growth in rice roots in space. Thus, growth and the cell wall properties of rice roots were strongly modified under microgravity conditions during spaceflight.

cell wall extensibility growth microgravity rice (Oryza sativa L.) root spaceflight 


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  1. Aarrouf J, Darbelley N, Demandre C, Razafindramboa N and Perbal G 1999 Effect of horizontal clinorotation on the root system development and on lipid breakdown in rapeseed (Brassica napus) seedlings. Plant Cell Physiol. 40, 396–405.PubMedGoogle Scholar
  2. Dubois M, Gilles K A, Hamilton J K, Rebers P A and Smith F 1956 Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356.Google Scholar
  3. Halstead T W and Dutcher F R 1987 Plants in space. Annu. Rev. Plant Physiol. 38, 317–345.PubMedGoogle Scholar
  4. Hilaire E, Paulsen A Q, Brown C S and Guikema J A 1995 Microgravity and clinorotation cause redistribution of free calcium in sweet clover columella cells. Plant Cell Physiol. 36, 831–837.PubMedGoogle Scholar
  5. Hoson T 1994 Automorphogenesis of maize roots under simulated microgravity conditions. Plant Soil 165, 309–314.Google Scholar
  6. Hoson T 1998 Apoplast as the site of response to environmental signals. J. Plant Res. 111, 167–177.PubMedGoogle Scholar
  7. Hoson T, Kamisaka S, Masuda Y and Yamashita M1992 Changes in plant growth processes under microgravity conditions simulated by a three-dimensional clinostat. Bot. Mag. 105, 53–70.Google Scholar
  8. Hoson T, Kamisaka S, Masuda Y, Yamashita M and Buchen B 1997 Evaluation of the three-dimensional clinostat as a simulator of weightlessness. Planta 203, S187–S197.PubMedGoogle Scholar
  9. Hoson T, Saiki M, Kamisaka S and Yamashita M 2001 Automorphogenesis and gravitropism of plant seedlings grown under microgravity conditions. Adv. Space Res. 27, 933–940.PubMedGoogle Scholar
  10. Hoson T, Soga K, Mori R, Saiki M, Nakamura Y, Wakabayashi K and Kamisaka S 2002 Stimulation of elongation growth and cell wall loosening in rice coleoptiles under microgravity conditions in space. Plant Cell Physiol. 43, 1067–1071.PubMedGoogle Scholar
  11. Hoson T, Soga K, Mori R, Saiki M, Wakabayashi K, Kamisaka S, Kamigaichi S, Aizawa S, Yoshizaki I, Mukai C, Shimazu T, Fukui K and Yamashita M 1999 Morphogenesis of rice and Arabidopsis seedlings in space. J. Plant Res. 112, 477–486.PubMedGoogle Scholar
  12. Johnsson A, Karlsson C, Iversen T H and Chapman D K 1996 Random root movements in weightlessness. Physiol. Plant. 96, 169–178.PubMedGoogle Scholar
  13. Kiss J Z, Edelmann R E and Wood P C 1999 Gravitropism of hypocotyls of wild-type and starch-deficient Arabidopsis seedlings in spaceflight studies. Planta 209, 96–103.PubMedGoogle Scholar
  14. Kordyum E L 1997 Biology of plant cells in microgravity and under clinostating. Int. Rev. Cytol. 171, 1–78.PubMedGoogle Scholar
  15. Levine L H, Heyenga A G, Levine H G, Choi J-W, Davin L B, Krikorian A D and Lewis N G 2001 Cell-wall architecture and lignin composition of wheat developed in a microgravity environment. Phytochemistry 57, 835–846.PubMedGoogle Scholar
  16. Lorenzi G and Perbal G 1990 Root growth and statocyte polarity in lentil seedling roots grown in microgravity or on a slowly rotating clinostat. Physiol. Plant. 78, 532–537.Google Scholar
  17. Masuda Y 1990 Auxin-induced cell elongation and cell wall changes. Bot. Mag. 103, 345–370.Google Scholar
  18. Masuda Y, Kamisaka S and Hoson T 1998 Growth behaviour of rice coleoptiles. J. Plant Physiol. 152, 180–188.Google Scholar
  19. Sakurai N 1991 Cell wall functions in growth and development – a physical and chemical point of view. Bot. Mag. 104, 235–251.Google Scholar
  20. Shibaoka H 1994 Plant hormone-induced changes in the orientation of cortical microtubules: alterations in the cross-linking between microtubules and the plasma membrane. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45, 521–544.Google Scholar
  21. Soga K, Wakabayashi K, Kamisaka S and Hoson T 2002 Stimulation of elongation growth and xyloglucan breakdown in Arabidopsis hypocotyls under microgravity conditions in space. Planta 215, 1040–1046.PubMedGoogle Scholar
  22. Tanimoto E 1988 Gibberellin regulation of root growth with change in galactose content of cell walls in Pisum sativum. Plant Cell Physiol. 29, 269–280.Google Scholar
  23. Tanimoto E, Fujii S, Yamamoto R and Inanaga S 2000 Measurement of viscoelastic properties of root cell walls affected by low pH in lateral roots of Pisum sativum L. Plant Soil 226, 21–28.Google Scholar
  24. Tanimoto E and Huber D J 1997 Effect of GA3 on the molecular mass of polyuronides in the cell walls of Alaska pea roots. Plant Cell Physiol. 38, 25–35.Google Scholar
  25. Volkmann D, Behrens H M and Sievers A 1986 Development and gravity sensing of cress roots under microgravity. Naturwissenschaften 73, 438–441.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Takayuki Hoson
    • 1
    Email author
  • Kouichi Soga
    • 1
  • Kazuyuki Wakabayashi
    • 1
  • Seiichiro Kamisaka
    • 1
  • Eiichi Tanimoto
    • 2
  1. 1.Department of Biology, Graduate School of ScienceOsaka City UniversitySugimoto, Sumiyoshi-ku, OsakaJapan
  2. 2.Graduate School of Natural SciencesNagoya City UniversityMizuho-ku, NagoyaJapan

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