Random walks with thin filaments: application of in vitro motility assay to the study of actomyosin regulation
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The in vitro motility devised by Kron and Spudich (Kron and Spudich, 1986; Kron et al., 1991) has proved a very valuable technique for studying the motor properties of myosin of all kinds but it is equally useful for the study of the thin filaments of muscle and their regulation. The movement of a population of thin filaments over immobilised myosin appears to be random but it does in fact yield a large amount of information about contractility and its regulation. The key to extracting useful information from in vitro motility assay experiments is the logical and comprehensive analysis of filament movements.
KeywordsMotility Assay Nemaline Myopathy Familial Hypertrophic Cardiomyopathy Muscle Tropomyosin Human Cardiac Troponin
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