Antonie van Leeuwenhoek

, Volume 84, Issue 4, pp 289–299 | Cite as

Evolutionary and structural diversity of fungal laccases

  • Brenda Valderrama
  • Patricia Oliver
  • Arturo Medrano-Soto
  • Rafael Vazquez-Duhalt


Fungal laccases have been extensively exploited for industrial purposes and there is a wealth of information available regarding their reaction mechanism, biological role and several molecular aspects, including cloning, heterologous expression and transcriptional analyses. Here we present the reconstruction of the fungal laccase loci evolution inferred from the comparative analysis of 48 different sequences. The topology of the phylogenetic trees indicate that a single monophyletic branch exists for fungal laccases and that laccase isozyme genes may have evolved independently, possibly through duplication-divergence events. Laccases are copper-containing enzymes generally identified by the utilization of substituted p-diphenol substrates. Interestingly, our approach permitted the assignment of two copper-containing oxidases, preliminarily catalogued as laccases, to a different evolutionary group, distantly related to the main branch of bona fide laccases.

Ascomycete Basidiomycete Laccase Multi copper oxidases White-rot fungi 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Brenda Valderrama
    • 1
  • Patricia Oliver
    • 1
  • Arturo Medrano-Soto
    • 2
  • Rafael Vazquez-Duhalt
    • 1
  1. 1.Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavaca, MorelosMexico
  2. 2.Centro de Investigación sobre Fijación de NitrógenoUniversidad Nacional Autónoma de MéxicoCuernavaca, MorelosMexico

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