Evolutionary and structural diversity of fungal laccases
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Fungal laccases have been extensively exploited for industrial purposes and there is a wealth of information available regarding their reaction mechanism, biological role and several molecular aspects, including cloning, heterologous expression and transcriptional analyses. Here we present the reconstruction of the fungal laccase loci evolution inferred from the comparative analysis of 48 different sequences. The topology of the phylogenetic trees indicate that a single monophyletic branch exists for fungal laccases and that laccase isozyme genes may have evolved independently, possibly through duplication-divergence events. Laccases are copper-containing enzymes generally identified by the utilization of substituted p-diphenol substrates. Interestingly, our approach permitted the assignment of two copper-containing oxidases, preliminarily catalogued as laccases, to a different evolutionary group, distantly related to the main branch of bona fide laccases.
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- Berka R.M., Schneider P., Golightly E.J., Brown S., Madden M., Brown K.M., Halkier T., Mondorf K. and Xu F.1997. Characterization of the gene encoding an extracellular laccase of Myceliophthora thermophila and analysis of the recombinant enzyme expressed in Aspergillus oryzae. Appl. Environ. Microbiol.63: 3151–3157.PubMedGoogle Scholar
- Felsenstein J.1989. PHYLIP–Phylogeny Inference Package (Version 3.2). Cladistics5: 164–166.Google Scholar
- Ferandez-Larrea J. and Stahl U.1996. Isolation and characterization of a laccase gene from Podospora anserina. Mol. Gen. Genet.252: 539–551.Google Scholar
- Genetics Computer Group 2002. Wisconsin Package Version 10.Google Scholar
- Gomez-Alarcon G., Lahoz R. and Molina D.1989. Production of extracellular enzymes during growth and autolysis of Pycnoporus cinnabarinus. J. Basic Microbiol.29: 23–29.Google Scholar
- Messerschmidt A., Rossi A., Ladenstein R., Huber R., Bolognesi M., Gatti G., Marchesini A., Petruzzelli R. and Finazzi-Agro A.1989. X-ray crystal structure of the blue oxidase ascorbate oxidase from zucchini. Analysis of the polypeptide fold and a model of the copper sites and ligands. J. Mol. Biol.206: 513–529.PubMedCrossRefGoogle Scholar
- Yaver D.S., Overjero M.D., Xu F., Nelson B.A., Brown K.M., Halkier T., Bernauer S., Brown S.H. and Kauppinen S.1999. Molecular characterization of laccase genes from the basidiomycete Coprinus cinereus and heterologous expression of the laccase lcc1. Appl. Environ. Microbiol.65: 4943–4948.PubMedGoogle Scholar
- Yaver D.S., Xu F., Golightly E.J., Brown K.M., Brown S.H., Rey M.W., Schneider P., Halkier T., Mondorf K. and Dalboge H.1996. Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa. Appl. Environ. Microbiol.62: 834–841.PubMedGoogle Scholar