Protein fraction of barley spent grain as a new simple medium for growth and sporulation of soil actinobacteria
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A cheap value-added product, the protein fraction of barley spent grains is proposed as a source of a potential and economical cultivation medium. We showed that medium composed of protein fraction extract allows the isolation of actinobacteria, especially Streptomyces, from soil samples, and enhances the sporulation. It was used for the screening and production of the biologically active substances from actinobacteria.
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- Cresswell N, Saunders VA, Wellington EMH (1991) Detection and quantification of Streptomyces violaceolatous plasmid DNA in soil. Lett. Appl. Microbiol. 13: 193–197.Google Scholar
- Hayakawa M, Nonomura H (1987) Efficacy of artificial humic acid as a selective nutrient in HV agar used for the isolation of soil actinomycetes. J. Ferment. Technol. 65: 609–616.Google Scholar
- Hayakawa M, Takeuchi T, Yamazaki T (1996) Combined use of trimethoprim with nalidixic acid for the selective isolation and enumeration of actinomycetes from soil. Actinomycetologica 10: 80–90.Google Scholar
- Ishiwaki N, Muruyama H, Awayama H, Kanauchi O, Sato T (2000) Development of high value uses of spent grain by fractionation technology. Tech. Quart. 37: 261–265.Google Scholar
- Ishizuka H, Horinouchi S, Kieser HM, Hopwood DA, Beppu T (1992) A putative two-component regulatory system involved in secondary metabolism in Streptomyces spp. J. Bacteriol. 174: 7585–7594.Google Scholar
- Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces Genetics. Norwich, UK: The John Innes Foundation.Google Scholar
- Lasztity R (1984). Barley proteins. In: The Chemistry of Cereal Proteins, CRC Press, pp. 117–126.Google Scholar
- Szwajcer Dey E, Rasmussen J, Breddam K (1992) Proline specific endopeptidases from microbial sources: isolation of an enzyme from Xanthomonas sp. J. Bacteriol. 174: 2454–2459.Google Scholar
- Takano E, Gramajo HC, Strauch E, Andres N, White J, Bibb MJ (1992) Transcriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2). Mol. Microbiol. 6: 2797–2804.Google Scholar
- Tsao SW, Rudd BA, He XG, Chang CJ, Floss HG (1985) Identification of a red pigment from Streptomyces coelicolor A3(2) as a mixture of prodigiosin derivatives. J. Antibiot. (Tokyo) 38: 128–131.Google Scholar
- Wellington EMH, Cresswell N, Saunders VA (1990) Growth and survival of streptomycete inoculants and the extent of plasmid transfer in sterile and non-sterile soil. Appl. Environ. Microbiol. 56: 1413–1419.Google Scholar