Biotechnology Letters

, Volume 25, Issue 20, pp 1709–1712 | Cite as

Antifreeze activities of poly(γ-glutamic acid) produced by Bacillus licheniformis


Various enantiomeric isomers, metals salts and molecular sizes of poly(γ-glutamic acid), γ-PGA, produced by Bacillus licheniformis CCRC 12826, were prepared and their antifreeze activities were studied by differential scanning calorimetry. The antifreeze activity of γ-PGA increased as its molecular weight decreased but was indifferent to its d/l-glutamate composition. The antifreeze activity was cation dependent decreasing in the order Mg2+>>Ca2+Na+>>K+ which follows that of inorganic chlorides in that high ionic charge leads to high antifreeze activity. The mechanism by which the cryoprotective effects of γ-PGA can be explained is still yet to be determined.

antifreeze activity Bacillus licheniformis poly(γ-glutamic acid) 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Department of Environmental EngineeringDa-Yeh UniversityChang-HwaTaiwan

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