Biology Bulletin of the Russian Academy of Sciences

, Volume 30, Issue 5, pp 464–467 | Cite as

Involvement of Oligosaccharides in Adaptation of Winter Wheat Seedlings to Subzero Temperatures

  • O. A. Zabotina
  • D. A. Ayupova
  • T. E. Toroshchina
  • A. I. Zabotin


We studied the dynamics of endogenous content of biologically active oligosaccharides in the roots of winter wheat seedlings. Previously, these oligosaccharides proved to mediate the development of frost resistance during the first days of hardening (Zabotinaet al., 1998). The changes in their endogenous content can be described by a curve with a single peak observed 6 h after the onset of frost hardening. The capacity of these polysaccharides to increase frost resistance (LT50was evaluated by leakage of electrolytes) when added to growth medium did not depend on the pretreatment duration (from 1.5 to 18 h) but decreased if they were introduced in the course of the adaptive response. Inhibition of the adaptive response by inhibitors of RNA and protein synthesis ceased in the presence of the oligosaccharides. We believe that the oligosaccharides that are products of metabolism of the cell wall polysaccharides are involved in adaptation to low temperature.


Cell Wall Polysaccharide Protein Synthesis Growth Medium Oligosaccharide 
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  1. Barysheva, T.S., Zabotina, O.A., and Zabotin, A.I., Effect of Cycloheximide on the Synthesis of Polysaccharides and Glycosidase Activities in Cell Walls of Wheat Roots during Cold Acclimation,Fiziol. Rast.(Moscow), 1999, vol. 456, pp. 633-638.Google Scholar
  2. Chaplin, M.F. and Kennedy, J.F.,Carbohydrate Analysis: A Practical Approach, IPL Press, 1988, p. 38.Google Scholar
  3. Graham, D. and Patterson, B.D., Responses of Plants to Low Nonfreezing Temperatures: Proteins, Metabolism and Acclimation,Annu. Rev. Plant Physiol., 1982, vol. 33, pp. 37-372.Google Scholar
  4. Guy, C.L., Cold Acclimation and Freezing Stress Tolerance: Role of Protein Metabolism,Annu. Rev. Plant Physiol. Mol. Biol., 1990, vol. 41, pp. 187-223.Google Scholar
  5. Guy, C.L. and Haskell, D., Induction of Freezing Tolerance in Spinach Is Associated with the Synthesis of Cold Acclimation-Induced Proteins,Plant Physiol., 1987, vol. 84, pp. 872-878.Google Scholar
  6. Knight, H., Trewavas, A.J., and Knight, M.R., Cold Calcium Signaling inArabidopsisInvolves Two Cellular Pools and a Change in Calcium Signature after Acclimation,Plant Cell1996, vol. 8, pp. 489-503.Google Scholar
  7. Larskaya, I.A. and Zabotin, A.I., Study of Time-Related Changes in cAMP Content during Low Temperature Adaptation,Tez. dokl. III Vseros. s''ezda fiziologov rastenii (Abstr. III All-Russia Conference of Plant Physiologists), 1993, p. 650.Google Scholar
  8. Thomashov, M.F., Molecular Genetics of Acclimation in Higher Plants,Adv. Genet., 1990, vol. 28, pp. 99-131.Google Scholar
  9. Titov, A.F., Talanov, V.V., and Drozdov, S.N., Effect of Specific Inhibitors of Transcription and Translation on Cold and Heat Hardening of Tomato,Fiziol. Rast.(Moscow), 1982, vol. 29, pp.790-793.Google Scholar
  10. Trunova, T.I., Winter Wheat Frost Hardiness and Protein Synthesis at Chilling Temperature, inPlant Cold Hardiness, Li, P., Ed., New York: Alan R. Liss, 1978, pp. 43-58.Google Scholar
  11. Veisz, O., Galoba, G., and Sutka, J., Effect of Abscisic Acid on the Cold Hardiness of Wheat Seedlings,J. Plant Physiol., 1991, vol. 149, nos.3-4, pp. 439-443.Google Scholar
  12. Zabotin, A.I., Barysheva, T.S., Zabotina, O.A., Larskaya, I.A., and Lozovaya, V.V., Plant Cell Wall and Development of the Hypothermia Syndrome,Dokl. Akad. Nauk SSSR1995, vol. 343, pp. 567-570.Google Scholar
  13. Zabotin, A.I., Barisheva, T.S., Zabotina, O.A., Larskaya, I.A., Lozovaya, V.V., Beldman, G., and Voragen, G.J., Alterations in Cell Walls of Winter Wheat Roots during Low Temperature Acclimation,J. Plant Physiol., 1998, vol. 152, pp. 473-479.Google Scholar
  14. Zabotina, O.A., Gur'yanov, O.P., Malikhov, R.G., Ayupova, D.A., Beldman, G., Voragen, A.Dzh., and Lozovaya, V.V., Effect of Oligosaccharides from Pea Shoots and Their Physiological Activity,Fiziol. Rast.(Moscow), 1995, vol. 42, pp. 416-422.Google Scholar
  15. Zabotina, O.A., Ayupova, D.A., Larskaya, I.A., Nikolaeva, O.G., Petrovicheva, G.A., and Zabotin, A.I., Physiologically Active Oligosaccharides Accumulating in the Roots of Winter Wheat during Adaptation to Low Temperature,Fiziol. Rast.(Moscow), 1998, vol. 45, pp. 262-267.Google Scholar
  16. Zvereva, G.N. and Trunova, T.I., Relationship between Frost Resistance of Winter Wheat and Protein Synthesis during Hardening,Fiziol. Rast.(Moscow), 1985, vol. 32, pp. 976-982.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • O. A. Zabotina
    • 1
  • D. A. Ayupova
  • T. E. Toroshchina
    • 1
  • A. I. Zabotin
    • 1
  1. 1.Russian Academy of SciencesKazan Institute of Biochemistry and BiophysicsKazanRussia

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