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Journal of Biomolecular NMR

, Volume 27, Issue 4, pp 341–349 | Cite as

Measurement of residual dipolar couplings from 1Hα to 13Cα and 15N using a simple HNCA-based experiment

  • Perttu Permi
Article

Abstract

Novel NMR pulse schemes for simultaneous measurement of 1DCαHαand 2DNHαresidual dipolar couplings in proteins is presented. We show that 2DNHαcoupling can be very useful for protein structure determination. The 2DNHαcoupling can be measured from 15N dimension with good accuracy on a slowly relaxing TROSY resonance, utilizing HNCA-TROSY-based experiments, which concomitantly supply large 1DCαHαcoupling. The dynamic range of 2DNHαcoupling is comparable to 1DNC′ coupling, but instead, it also serves non-redundant information on the course of protein backbone, thanks to rotational degree of freedom with respect to peptide bond. The HNCA-TROSY-based experiments are optimal for measuring residual dipolar couplings at high magnetic fields owing to absence of rapid transverse relaxation of carbonyl carbon. The reliability of the proposed approach was tested on 15N/13C human ubiquitin. A very good correlation with ubiquitin solution as well as crystal structure, for both 1DCαHαand 2DNHαcouplings, was obtained.

dipolar couplings HNCA NMR proteins scalar couplings spin-state-selection 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.NMR Laboratory, Structural Biology and Biophysics Programme, Institute of BiotechnologyUniversity of HelsinkiHelsinkiFinland

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