Behavior Genetics

, Volume 27, Issue 1, pp 55–66

Short-Term Selective Breeding as a Tool for QTL Mapping: Ethanol Preference Drinking in Mice

  • J. K. Belknap
  • S. P. Richards
  • L. A. O'Toole
  • M. L. Helms
  • T. J. Phillips
Article

DOI: 10.1023/A:1025615409383

Cite this article as:
Belknap, J.K., Richards, S.P., O'Toole, L.A. et al. Behav Genet (1997) 27: 55. doi:10.1023/A:1025615409383

Abstract

Short-term selective breeding starting from an F2 intercross of two inbred strains is a largely unexploited but potentially useful tool for quantitative trait locus (QTL) mapping. The selection lines can also serve as a valuable confirmation test of recornbinant inbred (RI) QTL results when the same two progenitor strains are used. Starting from an F2 from a C57BL/6J (B6) × DBA/2J (D2) cross (B6D2F2), this approach was used in a population of ~72 mice per generation bidirectionally selected for two-bottle choice 10% ethanol (alcohol) preference for four generations. The high-preference line diverged significantly from the low line in the first generation with a realized heritabittty of .32. By generation 4, the preference ratios in the high line were double those seen in the low line. Regions of the genome previously implicated by BXD RI QTL analysis as containing QTLs were searched using microsatellite markers. The test for the presence of QTLs was based on the divergence of marker allele frequencies in the two oppositely selected lines significantly exceeding that expected from random (genetic) drift and allele frequency estimation error. Combining the BXD and two-way selection line results, the most probable QTL was found on chromosome 3 (near the AdhI locus; LOD ~2.9), other probable QTLs were found with LOD 2.4–2.6.

Quantitative trait locus (QTL) C57BL/6J DBA/2J ethanol preference alcohol drinking gene mapping selective breeding mouse inbred strains 

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • J. K. Belknap
    • 1
  • S. P. Richards
    • 1
  • L. A. O'Toole
    • 1
  • M. L. Helms
    • 1
  • T. J. Phillips
    • 1
  1. 1.Research Service (151W), VA Medical Center, and Department of Behavioral NeuroscienceOregon Health Sciences UniversityPortland

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