Behavior Genetics

, Volume 27, Issue 3, pp 251–257 | Cite as

DNA by Mail: An Inexpensive and Noninvasive Method for Collecting DNA Samples from Widely Dispersed Populations

  • Bernard Freeman
  • John Powell
  • David Ball
  • Linzy Hill
  • Ian Craig
  • Robert Plomin
Article

Abstract

As specific genes are identified that are associated with behavior, it becomes increasingly important for behavioral geneticists to be able to incorporate these genes in their research. Rather than using blood, DNA can be extracted from cheek swabs, which makes it possible to obtain DNA inexpensively by mail from large, widely dispersed individuals. The purpose of this paper is to recommend this technique to the behavioral genetics community and to present results of our use of this technique to obtain DNA by mail for 114 2-year-olds and 116 adults.

Genes DNA genotyping behavioral genetics mail 

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REFERENCES

  1. Edwards, A., Civitello, A., Hammond, H. A., and Caskey, C. T. (1991a). DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am. J. Hum. Genet. 49:746–756.Google Scholar
  2. Edwards, M. C., Clemens, P. R., Tristan, M., Pizzuti, A., and Gibbs, R. A. (1991b). Pentanucleotide repeat length polymorphism at the human CD4 locus. Nucleic Acids Res. 19:4791.Google Scholar
  3. Gyapay, G., Morissette, J., Vignal, A., Dib, C., Fizames, C., Millasseau, P., Marc, S., Bernardi, G., Lathrop, M., and Weissenbach, J. (1994). The 1993–1994 Genethon Human Genetic Linkage Map. Nature Genet. 7:246–339.Google Scholar
  4. Hata, A., and Lalouel, J.-M. (1991). Micro VNTR (CTTT)n at the apo C-III locus. Nucleic Acids Res. 19:5098.Google Scholar
  5. Kimpton, C. P., Gill, P., Walton, A., Urquhart, A., Millican, E. S., and Adams, M. (1993). Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR Methods Appl. 3:13–22.Google Scholar
  6. McGuffin, P., Owen, M. J., O'Donovan, M. C., Thapar, A., and Gottesman, I. I. (1994). Seminars in Psychiatric Genetics, Gaskell Press, London.Google Scholar
  7. Meulenbelt, I., Droog, S., Trommelen, G. J., Boomsma, D. I., and Slagboom, P. E. (1995). High yield noninvasive human genomic DNA isolation method for genetic studies in geographically dispersed families and populations. Am. J. Hum. Genet. 57:1252–1254. ai]Plomin, R., DeFries, J. C., McClearn, G. E., and Rutter, M. (1997). Behavioral Genetics, 3rd ed., W. H. Freeman, New York.Google Scholar
  8. Plomin, R., and Rutter, M. (1997). Child development and molecular genetics: What do we do with genes once they are found? Child Dev. (in press).Google Scholar
  9. Plomin, R., Owen, M. J., and McGuffin, P. (1994). The genetic basis of complex human behaviors. Science 264:1733–1739.Google Scholar
  10. Polymeropoulos, M. H., Rath, D. S., Xiao, H., and Merril, C. R. (1990a). Trinucleotide repeat polymorphism at the human intestinal fatty acid binding protein gene (FABP2). Nucleic Acids Res. 18:7198.Google Scholar
  11. Polymeropoulos, M. H., Rath, D. S., Xiao, H., and Merril, C. R. (1990b). Trinucleotide repeat polymorphism at the human pancreatic phospholipase A-2 gene (PLA2). Nucleic Acids Res. 18:7468.Google Scholar
  12. Polymeropoulos, M. H., Xiao, H., Rath, D. S., and Merril, C. R. (1991a). Tetranucleotide repeat polymorphism at the human aromatase cytochrome P-450 gene (CYP19). Nucleic Acids Res. 19:195.Google Scholar
  13. Polymeropoulos, M. H., Xiao, H., Rath, D. S., and Merril, C. R. (1991b). Tetranucleotide repeat polymorphism at the human tyrosine hydroxylase gene (TH). Nucleic Acids Res. 19:3753.Google Scholar
  14. Polymeropoulos, M. H., Xiao, H., Rath, D. S., and Merril, C. R. (1991c). Tetranucleotide repeat polymorphism at the human dihydrofolate reductase psi-2 pseudogene (DHFRP2). Nucleic Acids Res. 19:4792.Google Scholar
  15. Richards, B., Skoletsky, J., Shuber, A. P., Balfour, R., Stern, R. C., Dorkin, H. L., Parad, R. B., Witt, D., and Klinger, K. W. (1993). Multiplex PCR amplification from the CFTR gene using DNA prepared from buccal brushes/swabs. Hum. Mol. Genet. 2:159–163.Google Scholar
  16. Riemann, R., and Angleitner, A. (1997). Genetic and environmental influences on personality: A study of twins reared together using the self-and peer report NEO-FFI scales. J. Pers. (in press).Google Scholar
  17. Zulliano, G., and Hobbs, H. (1990). Tetranucleotide repeat polymorphism in the op-lipoprotein C-III gene. Nucleic Acids Res. 18:4299.Google Scholar

Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Bernard Freeman
    • 1
  • John Powell
    • 1
  • David Ball
    • 1
  • Linzy Hill
    • 1
  • Ian Craig
    • 1
  • Robert Plomin
    • 1
  1. 1.Social, Genetic and Developmental Psychiatry Research CentreInstitute of PsychiatryLondonUnited Kingdom

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