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Biotechnology Letters

, Volume 25, Issue 18, pp 1509–1514 | Cite as

Genetic transformation of Monascus purpureus DSM1379

  • Jeong-Gu Kim
  • Yang Do Choi
  • Yung-Jin Chang
  • Soo-Un Kim
Article

Abstract

Monascus purpureus was transformed into hygromycin B resistance with hygromycin B phosphotransferase (hph) fused to Aspergillus nidulans trpC or a putative Monascus purpureus gpd1 promoter by electroporation. Among five strains, only M. purpureus DSM1397 was a competent recipient. Normal growth and sporulation on media containing up to 500 mg hygromycin B l−1 occurred up to five generations. Upon transformation of the strain with the green fluorescent protein gene (sgfp) as a model gene and hph as a selection marker, characteristic green fluorescence was observed under fluoromicroscopy indicating successful transformation.

electroporation glyceraldehyde-3-phosphate dehydrogenase promoter green fluorescent protein hygromycin B resistance Monascus purpureus 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Jeong-Gu Kim
    • 1
  • Yang Do Choi
    • 2
  • Yung-Jin Chang
    • 2
  • Soo-Un Kim
    • 2
    • 3
  1. 1.Bioinformatics DivisionNational Institute of Agricultural Biotechnology, Rural Development AdministrationSuwonKorea
  2. 2.School of Agricultural Biotechnology, College of Agriculture and Life SciencesSeoul National UniversitySuwonKorea
  3. 3.Plant Metabolism Research CenterKyung Hee UniversityYonginKorea

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