Immunogenicity of Ehrlichia ruminantium Grown in Tick Cell Lines
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Ehrlichia (previously Cowdria) ruminantium, the pathogen which causes heartwater in domestic and wild ruminants, can now be propagated in cell lines from one vector (Amblyomma variegatum) and five non-vector (Ixodes scapularis, I. ricinus, Boophilus decoloratus, B. microplus and Rhipicephalus appendiculatus) tick species. E. ruminantium isolates from West and South Africa and the Caribbean vary in their cell line preference, growth patterns and immunogenic capability. In laboratory trials, certain combinations of tick cell line and E. ruminantium isolate were highly immunogenic in sheep. These trial vaccines were grown under specific in vitro conditions and administered as a single intravenous dose of freshly harvested whole, live culture. Following immunisation and subsequent exposure to virulent E. ruminantium, protected sheep showed no clinical response and a range of serological responses.
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- Bell, L.J. 1983. Development of Theileria in tick tissue culture. MPhil Thesis, University of Edinburgh, 236 pp.Google Scholar
- Bell-Sakyi, L., Koney, E.B.M., Dogbey, O., Abbam, J.A. and Aning, K.G. 1997. Isolation and in vitro cultivation in Ghana of Cowdria ruminantium, the causative agent of heartwater. In: Proceedings of the W.A.C.V.A/G.V.M.A. Conference, E.B.M. Koney and K.G. Aning (eds), September 1996, pp. 46-51, Accra, Ghana.Google Scholar
- Bell-Sakyi, L., Paxton, E.A., Munderloh, U.G. and Sumption, K.J. 2000a. Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line. J. Clin. Microbiol. 8: 1238-1240.Google Scholar
- Bell-Sakyi, L., Paxton, E.A., Munderloh, U.G. and Sumption, K.J. 2000b. Morphology of Cowdria ruminantium grown in two tick cell lines pp. 131-137. In: Proceedings of the 3rd International Conference “Ticks and Tick-borne Pathogens: Into the 21st Century”, M. Kazimirova, M. Labuda, P.A. Nuttall (eds), Institute of Zoology, Slovak Academy of Sciences, Bratislava, Slovakia.Google Scholar
- Bezuidenhout, J.D., Paterson, C.L. and Barnard, B.J.H. 1985. In vitro cultivation of Cowdria ruminantium. Onderstepoort J. Vet. Res. 51: 113-120.Google Scholar
- Camus, E., Barre, N., Martinez, D. and Uilenberg, G. 1996. Heartwater (cowdriosis) a Review, 2nd edn., 177 pp. O.I.E., Paris.Google Scholar
- Dumler, J.S., Barbet, A.F., Bekker, C.P.J., Dasch, G.A., Palmer, G.H., Ray, S.C., Rikihisa, Y. and Rurangirwa, F.R. 2001. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int. J. Syst. Evol. Microbiol. 51: 2145-2165.PubMedGoogle Scholar
- Ewing, S.A., Munderloh, U.G., Blouin, E.F., Kocan, K.M. and Kurtti, T.J. 1995. Ehrlichia canis in tick cell culture. In: Proceedings of the 76th Conference of Research Workers in Animal Diseases, Chicago, USA, 13-14 November 1995. Iowa State University Press, Ames, Iowa, USA.Google Scholar
- Martinez, D. 1997. Analysis of the immune response of ruminants to Cowdria ruminantium infection. PhD Thesis, University of Utrecht, 206 pp.Google Scholar
- Mboloi, M.M., Bekker, C.P.J., Kruitwagen, C., Greiner, M. and Jongejan, F. 1999. Validation of the indirect MAP1-B enzyme-linked immunosorbent assay for diagnosis of experimental Cowdria ruminantium infection in small ruminants. Clin. Diag. Lab. Immunol. 6: 66-72.Google Scholar
- Munderloh, U.G., Jauron, S.D., Fingerle, V., Leitritz, L., Hayes, S.F., Hautman, J.M., Nelson, C.M., Huberty, B.W., Kurtti, T.J., Ahlstrand, G.G., Greig, B., Mellencamp, M.A. and Goodman, J.L. 1999. Invasion and intracellular development of the human granulocytic ehrlichiosis agent in tick cell culture. J. Clin. Microbiol. 37: 2518-2524.PubMedGoogle Scholar