Prevalence and Antimicrobial Resistance Pattern of Salmonella Isolates from Apparently Healthy Slaughtered Cattle in Ethiopia

  • D. Alemayehu
  • B. Molla
  • A. Muckle


The prevalence and antimicrobial resistance pattern of Salmonella isolates was determined from apparently healthy slaughtered cattle at Debre Zeit (Ethiopia). A total of 323 cattle were examined for the presence of Salmonella in faeces, mesenteric lymph nodes, abdominal and diaphragmatic muscles. Salmonellae were cultured from 23 (7.1%) of the animals. Salmonellae were isolated from 2 (3.1%) and 3 (4.5%) of 65 pooled faecal and mesenteric lymph node samples, respectively. Nine (2.8%) abdominal muscle and 10 (3.1%) diaphragmatic muscle samples (n = 323 of each) were contaminated by Salmonella. About 60% of the serovars identified in the abdominal and diaphragmatic muscles were also detected from faeces and mesenteric lymph node samples. The five different serovars isolated were Salmonella mishmarhaemek (48%), S. typhimurium (20%), S. enteritidis (12%), S. guildford (12%) and S. dublin (48%). The antimicrobial resistance profiles of 25 of the Salmonella isolates with 17 antimicrobials showed that 52% (13/25) of them were resistant to three or more antimicrobials. Both strains of Salmonella (S. mishmarhaemek and S. typhimurium) showed multiple resistance to ampicillin, sulfamethoxazole and ticarcillin.

antibiotics cattle drug resistance epidemiology Salmonella serovars 


  1. Acha, P.N. and Szyfres, B., 1989. Zoonoses and Communicable Diseases Common to Man and Animals, 2nd edn, (PAHO, Washington DC)Google Scholar
  2. Adesiyun, A.A. and Oni, O.O., 1989. Prevalence and antibiograms of salmonellae in slaughtered cattle, slaughter areas and effluents in Zaria abattoir, Nigeria. Journal of Food Protection, 52, 232-235Google Scholar
  3. Anderson, E.S., 1964. The phagetyping of Salmonella other than S. typhi. In: E. Van Oye (ed.), The World Problem of Salmonellosis, (Dr W. Junk Publishers, The Hague)Google Scholar
  4. Anderson, E.S. and Williams, R.E.O., 1956. Bacteriophage typing of enteric pathogens and staphylococci and its use in epidemiology. Journal of Clinical Pathology, 9, 94-114PubMedGoogle Scholar
  5. Anderson, E.S., Ward, L.R., De Saxe, M.J., De Sa., J.D.H., 1977. Bacteriophage-typing designations of Salmonella typhimurium. Journal of Hygiene, 78, 297-300PubMedGoogle Scholar
  6. Bennett, J.V., 1980. Antibiotic use in animals and human salmonellosis. Journal of Infectious Diseases, 142, 631-633PubMedGoogle Scholar
  7. Callow, B.R., 1959. A new phagetyping scheme for Salmonella typhimurium. Journal of Hygiene, 57, 346-359PubMedCrossRefGoogle Scholar
  8. D'Aoust, J.-Y., 1989. Salmonella. In: M.P. Doyle (ed.), Foodborne Bacterial Pathogens, (Marcel Dekker, New York)Google Scholar
  9. D'Aoust, J.-Y., Sewell, A.M., Daley, E. and Greco, P., 1992. Antibiotic resistance of agricultural and foodborne Salmonella isolates in Canada. Journal of Food Protection, 55, 428-434Google Scholar
  10. Duffy, G., Cloak, O.M., O'Sullivan, M.G., Guillet, A., Sheridan, J.J., Blair, I.S. and McDowell, D.A., 1999. The incidence and antibiotic resistance profiles of Salmonella spp. on Irish retail meat products. Food Microbiology, 16, 623-631CrossRefGoogle Scholar
  11. Ewing, W.H., 1986. Serologic identification of Salmonella. In: W.H. Ewing (ed.), Edwards and Ewing's Identification of Enterobacteriaceae, 4th edn, (Elsevier Science, New York)Google Scholar
  12. Fedorka-Cray, P.J., Dargatz, D.A., Thomas, L.A. and Gray, J.T., 1998. Survey of Salmonella serovars in feedlot cattle. Journal of Food Protection, 61, 525-530PubMedGoogle Scholar
  13. Gay, J.M., Rice, D.H. and Steiger, J.H., 1994. Prevalence of faecal Salmonella shedding by cull dairy cattle marketed in Washington State. Journal of Food Protection, 57, 195-197Google Scholar
  14. Gustafson, R.H. and Bowen, R.E., 1997. Antibiotic use in animal agriculture. Journal of Applied Microbiology, 83, 531-541PubMedCrossRefGoogle Scholar
  15. Hadfield, T.L., Monson, M.H. and Wachsmuth, I.K., 1985. An outbreak of antibiotic resistant Salmonella enteritidis in Liberia, West Africa. Journal of Infectious Diseases, 151, 790-795PubMedGoogle Scholar
  16. House, J.K. and Smith, B.P., 1998. Current strategies for managing Salmonella infections in cattle. Veterinary Medicine, 93, 756-764Google Scholar
  17. Hummel, P.H., 1979. Antibiotic resistance among salmonellae isolated from animals in Tanzania. Bulletin of Animal Health and Production in Africa, 27, 113-121Google Scholar
  18. ISO 6579, 1998. Microbiology of Food and Animal Feeding Stuff — Horizontal Method for the Detection of Salmonella, (International Organization for Standardization, Geneva)Google Scholar
  19. Leegaard, T.M., Van Gestel, M.H., Petit, P.L.C. and Van De Klundet, J.A.M., 1996. Antibiotic resistance mechanisms in Salmonella species causing bacteraemia in Malawi and Kenya. Acta Pathologica Microbiologica et Immunobiologica Scandinavica (APMIS), 104, 302-306Google Scholar
  20. Le Minor, L. and Popoff, M.Y., 1992. Antigenic Formulas of the Salmonella Serovars, 6th edn, (WHO Collaborating Centre for Reference and Research on Salmonella, Paris)Google Scholar
  21. Mache, A. and Mengistu, Y., 1998. Salmonella serogroups identified from adult diarrhoeal outpatients in Addis Ababa, Ethiopia: antibiotic resistance and plasmid profile analysis. The 7th Annual Conference of the Biological Society of Ethiopia, Faculty of Science, Addis Ababa UniversityGoogle Scholar
  22. Miller, A.S., 1971. Salmonellosis in Botswana: I. Incidence in cattle. Journal of Hygiene (Cambridge), 69, 491-495Google Scholar
  23. Molla, B., Kleer, J. and Sinell, H.-J., 1999a. Occurrence, distribution and level of Salmonella in selected food items in Addis Ababa (Ethiopia). Fleischwirtschaft International, 4, 37-39Google Scholar
  24. Molla, B., Kleer, J. and Sinell, H.-J., 1999b. Antibiotic resistance pattern of foodborne Salmonella isolates in Addis Ababa (Ethiopia). Berliner und Münchener Tierärztliche Wochenschrift, 112, 41-43PubMedGoogle Scholar
  25. Molomo, M.A., 1998. A study on prevalence and epidemiological key factors of Salmonella species in selected poultry farms in Debre-Zeit, Ethiopia, (MSc thesis, Addis Ababa University and Free University of Berlin)Google Scholar
  26. Motarjemi, Y., Kaeferstein, F., Moy, G., Miyagishima, K., Miyagawa, S. and Reilly, A., 1995. Food Technologies and Public Health, (Food Safety Issues, WHO/FNUF/FOS/95.12)Google Scholar
  27. NCCLS, 1990. Performance Standards for Antimicrobial Susceptibility Tests, 3rd edn, Approved Standard M2-A3, (National Committee for Clinical Laboratory Standards, Villanova, PA)Google Scholar
  28. Nyeleti, C., 1999. Epidemiological study and zoonotic importance of bovine salmonellosis in selected sites of Addis Ababa, Ethiopia, (MSc thesis, Addis Ababa University and Free University of Berlin)Google Scholar
  29. Oosterom, J., 1991. Epidemiological studies and proposed preventive measures in the fight against human salmonellosis. International Journal of Food Microbiology, 12, 41-52PubMedCrossRefGoogle Scholar
  30. Opuda-Asibo, J., Robinson, R.A. and Pullen, M.M., 1990. Prevalence of Salmonella in healthy calves following transporation to the stockyards and at slaughter. Bulletin of Animal Health and Production in Africa, 38, 101-102Google Scholar
  31. Puyalto, C., Colmin, C. and Laval, A., 1997. Salmonella typhimurium contamination from farm to meat in adult cattle: descriptive study. Veterinary Research, 28, 449-460PubMedGoogle Scholar
  32. Quinn, P.J., Carter, M.E., Markey, B., Carter, G.R., 1994. Clinical Veterinary Microbiology, (Wolfe, London)Google Scholar
  33. Samuel, J.L., O'Boyle, D.A., Mathers, W.J. and Frost, A.J., 1979. Isolation of Salmonella from mesenteric lymph nodes of healthy cattle at slaughter. Research in Veterinary Science, 28, 238-241Google Scholar
  34. Samuel, J.L., O'Boyle, D.A., Mathers, W.J. and Frost, A.J., 1980. Distribution of Salmonella in the carcasses of normal cattle at slaughter. Research in Veterinary Science, 28, 368-372PubMedGoogle Scholar
  35. Shipp, C.R. and Rowe, B., 1980. A mechanised microtechnique for Salmonella serotyping. Journal of Clinical Pathology, 33, 595-597PubMedGoogle Scholar
  36. Tauxe, R.V., 1991. Salmonella: a postmodern pathogen. Journal of Food Protection, 54, 563-568Google Scholar
  37. Ward, L.R., De Sa, J.D.H. and Rowe, B., 1987. A phage-typing scheme for Salmonella enteritidis. Epidemiology and Infection, 99, 291-294PubMedCrossRefGoogle Scholar
  38. Williams, D.R. and Bellhouse, R., 1978. The prevalence of salmonellae in healthy cattle at slaughter. The Veterinary Record, 103, 359-360PubMedGoogle Scholar
  39. Wray, C. and Davies, R.H., 2000. Salmonella infections in cattle. In: C. Wray and A. Wray (eds), Salmonella in Domestic Animals, (CABI Publishing, New York)Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • D. Alemayehu
    • 1
  • B. Molla
    • 1
  • A. Muckle
    • 2
  1. 1.Department of Microbiology, Infectious Diseases and Veterinary Public Health, Faculty of Veterinary MedicineAddis Ababa UniversityDebre ZeitEthiopia
  2. 2.OIE Reference Laboratory for Salmonellosis, Health Canada Laboratory for Foodborne ZoonosesOntarioCanada

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