Virus Genes

, Volume 27, Issue 1, pp 11–16

Sequence Analysis of the 3′ end of Three Grapevine Fleck Virus-like Viruses from Grapevine

  • Nina Abou Ghanem-Sabanadzovic
  • Sead Sabanadzovic
  • Giovanni P. Martelli
Article

Abstract

The 3′ end of the genome of three Grapevine fleck virus-like viruses, i.e. Grapevine redglobe virus (GRGV), Grapevine asteroid mosaic-associated virus (GAMaV), and an unidentified virus from a Greek grapevine (accession GR8-19) was amplified from reverse transcribed total nucleic acid extracts from infected grapevine tissues and sequenced. The analysed genome portions differed in size and organization. The 3′ ends of GAMaV (1852 nt) and of GR8-19 (1791 nt) resembled that of marafiviruses, as both encoded a single putative polyprotein containing the conserved “marafibox” sequence and lacked the stop codon between the replicase and coat protein genes. By contrast, the replicase and coat protein genes present in the terminal 2006 nt of GRGV genome were clearly separated and there was a 3′-proximal open reading frame encoding a putative proline rich protein with molecular mass of c. 17 kDa. The genome of all three viruses was polyadenylated. The organization of the 3′ terminal genomic region and phylogenetic analysis of viral replicases and coat proteins suggest that GAMaV and the Greek virus GR8-19 belong in the genus Marafivirus, and GRGV in the genus Maculavirus, family Tymoviridae. Virus GR8-19 had molecular traits differing enough from GAMaV and other marafiviruses to be regarded as a new putative species in the genus Marafivirus, for which the name of Grapevine rupestris vein feathering virus is proposed.

grapevine phylogenetic analysis sequencing taxonomy Tymoviridae 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sabanadzovic S., Abou Ghanem-Sabanadzovic N., Saldarelli P., and Martelli G.P., J Gen Virol 82, 2009-2015, 2001.Google Scholar
  2. 2.
    Martelli G.P., Sabanadzovic S., Abou Ghanem-Sabanadzovic N., and Saldarelli P., Arch Virol 147, 1847-1853, 2002.Google Scholar
  3. 3.
    Martelli G.P., Sabanadzovic S., Abou Ghanem-Sabanadzovic N., Edwards M.C., and Dreher T., Arch Virol 147, 1837-1846, 2002.Google Scholar
  4. 4.
    Kyriakopoulou P.E., Tzortzakakis E.A., and Tsagris M., Ext abstr 11 Meeting ICVG, Montreux, 41, 1993.Google Scholar
  5. 5.
    El Beaino T., Sabanadzovic S., Digiaro M., Abou Ghanem-Sabanadzovic N., Rowhani A., Kyriakpouolou P.E., and Martelli G.P., Vitis 40, 65-68, 2001.Google Scholar
  6. 6.
    Sabanadzovic S., Abou-Ghanem N., Castellano M.A., Digiaro M., and Martelli G.P., Arch Virol 145, 553-565, 2000.Google Scholar
  7. 7.
    Boscia D., Sabanadzovic S., Savino V., Kyriakopoulou P.E., Martelli G.P., and Lafortezza R., Vitis 33, 101-102, 1994.Google Scholar
  8. 8.
    Foissac X., Svanella-Dumas L., Dulucq M.J., Candresse T., and Gentit P., Acta Hort 550, 37-44, 2001.Google Scholar
  9. 9.
    Marck C., Nucleic Acids Res 16, 1829-1836, 1988.Google Scholar
  10. 10.
    Thompson J.D., Higgins D.G., and Gibson T.J., Nucleic Acids Res 22, 4673-4680, 1994.Google Scholar
  11. 11.
    Page R.D.M., Computer Appl Biosci 12, 357-358, 1996.Google Scholar
  12. 12.
    Idzapanah K., Zhang Y.P., Daubert S., and Rowhani A., Virus Genes 24, 131-134, 2002.Google Scholar
  13. 13.
    Ding S.W., Howe J., Keese P., MacKenzie A., Meek D., Osorio-Keese M., Skotnicki M., Srifah P., Torronen M., and Gibbs A.J., Nucleic Acids Res 18, 1181-1187, 1990.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Nina Abou Ghanem-Sabanadzovic
    • 1
  • Sead Sabanadzovic
    • 1
  • Giovanni P. Martelli
    • 2
  1. 1.Istituto Agronomico MediterraneoValenzano (Bari)Italy
  2. 2.Dipartimento di Protezione delle piante e Microbiologia ApplicataUniversità degli Studi and Istituto di Virologia Vegetate del CNRsezione di BariItaly

Personalised recommendations