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Degradation of a tannery and textile dye, Navitan Fast Blue S5R by Pseudomonas aeruginosa

  • C. Valli Nachiyar
  • G. Suseela Rajkumar
Article

Abstract

The degradation of Navitan Fast Blue S5R, a very important commercial diazo dye in the tannery and textile industries was investigated. Pseudomonas aeruginosa decolourized this dye at concentrations upto 1200 mg l−1 and the organism was also able to decolourize various other tannery dyes at different levels. The organism required ammonium salts and glucose to co-metabolize the dye. Organic nitrogen sources did not support appreciable decolourization whereas, combined with inorganic nitrogen (NH4NO3) there was an increased effect on both growth and decolourization. Decolourization of this dye started when the organism reached late exponential growth phase and after 24 h of incubation nearly 90% of 100 mg l−1 of the dye was decolourized. An oxygen insensitive azoreductase was involved in the decolourization mechanism. HPLC analysis confirmed the formation of metanilic acid from the dye, which on further incubation was completely metabolized under shaken culture condition.

Azoreductase degradation kinetics metanilic acid Navitan Fast Blue S5R Pseudomonas aeruginosa 

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References

  1. Abubacker, M.N., Anbu, P., Hilda, A. & Senthil Kumar, S. 2001 Cellulose biodegradation. Potential of some fungi. Asian Journal of Microbiology, Biotechnology and Environmental Science 3, 49–51.Google Scholar
  2. Catino, S.G. & Farris, R.E. 1978 Azo dyes. In Kirk-Othmer Encyclopedia of Chemical Technology, 3rd edn. vol. 3, pp. 387–433. New York: Wiley-Interscience. ISBN 0–471–02039–7.Google Scholar
  3. Chung, K.T. 1983 The significance of azoreduction in the mutagenesis and carcinogenesis of azo dyes. Mutation Research 114, 269–281.PubMedGoogle Scholar
  4. Chung, K.T., Fulk, G.E. & Andrews, A.W. 1981 Mutagenicity testing of some commonly used dyes. Applied and Environmental Microbiology 42, 641–648.PubMedGoogle Scholar
  5. Coughlin, M.F., Kinkle, B.K. & Bishop, P.L. 1999 Degradation of azo dyes containing aminonaphthol by Sphingomonas sp. Strain 1CX. Journal of Industrial Microbiology and Biotechnology 23, 341–346.PubMedGoogle Scholar
  6. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.H. & Smith, F. 1956 Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–356.Google Scholar
  7. Knapp, J.S., Zhang, F. & Tapley, K.N. 1997 Decolourisation of Orange II by a wood-rotting fungus. Journal of Chemical Technology and Biotechnology 69, 289–296.Google Scholar
  8. Kulla, H.G. 1981 Aerobic bacterial degradation of azo dyes. In Microbial Degradation of Xenobiotic and Recalcitrant Compounds, eds. Leisinger, T., Hutter, R., Cook, A.M. & Nuesch, J. pp. 387–399. London: Academic Press. ISBN 0–12–442920–3.Google Scholar
  9. Kulla, H.G., Klausener, F., Meyer, U., Ludeke, B. & Leisinger, T. 1983 Interference of aromatic sulfo groups in the microbial degradation of azo dyes Orange I and Orange II. Archives of Microbiology 135, 1–7.Google Scholar
  10. Meyer, U. 1981 Biodegradation of synthetic organic colourants. In Microbial Degradation of Xenobiotic and Recalcitrant Compounds, eds. Leisinger, T., Hutter, R., Cook, A.M. & Nuesch, J. pp. 371–385. London: Academic Press. ISBN 0–12–442920–3.Google Scholar
  11. Michaels, G.B. & Lewis, D.L. 1986 Microbial transformation rates of azo and triphenylmethane dyes. Environmental Toxicology and Chemistry 5, 161–166.Google Scholar
  12. Paszczynski, A., Pasti-Grigsby, M.B., Goszczynski, S., Crawford, R.L. & Crawford, D.L. 1992 Mineralization of sulfonated azo dyes and sulfanilic acid by Phanerochaete chrysosporium and Streptomyces chromofuscus. Applied and Environmental Microbiology 58, 3598–3604.PubMedGoogle Scholar
  13. Rafii, F., Moore, J.D., Ruseler-van Embden, J.G.H. & Cerniglia, C.E. 1995 Bacterial reduction of azo dyes used in foods, drugs and cosmetics. Microecology and Therapy 25, 147–156.Google Scholar
  14. Rajaguru, P., Kalaiselvi, Palanivel, M. & Subburam, V. 2000 Biodegradation of azo dyes in a sequential anaerobic-aerobic system. Applied Microbiology and Biotechnology 54, 268–273.PubMedGoogle Scholar
  15. Reife, A., Betowski, D. & Freeman, H.S. 1998 Dyes and pigments, environmental chemistry. In Encyclopedia of Environmental Analysis and Remediation, ed. Meyers, R.A. vol. B. pp. 1442–1465. New York: Wiley Interscience. ISBN 0–471–117–8–0.Google Scholar
  16. Sankar, M., Sekaran, G., Sadulla, S. & Ramasami, T. 1999 Removal of diazo and triphenylmethane dyes from aqueous solutions through an adsorption process. Journal of Chemical Technology and Biotechnology 74, 337–344.Google Scholar
  17. Spadaro, J.T. & Renganathan, V. 1994 Peroxidase-catalyzed oxidation of azo dyes: mechanism of disperse yellow 3 degradation. Archieves of Biochemistry and Biophysics 312, 301–307.Google Scholar
  18. Stolz, A. 2001 Basic and applied aspects in the microbial degradation of azo dyes. Applied Microbiology and Biotechnology 56, 69–80.PubMedGoogle Scholar
  19. Suseela, R.G., Ramesh, R. & Nandy, S.C. 1991 Degradation of PCP by Pseudomonas aeruginosa. Indian Journal of Environmental Health 33, 425–432.Google Scholar
  20. Tan, N.C.G., Prenafeta-Boldu, F.X., Opsteeg, J.L., Lettinga, G. & Field, J.A. 1999 Biodegradation of azo dyes in cocultures of anaerobic granular sludge with aerobic aromatic amine degrading enrichment cultures. Applied Microbiology and Biotechnology 51, 865–871.PubMedGoogle Scholar
  21. Yatome, C., Ogawa, T., Koga, D. & Idaka, E. 1981 Biodegradability of azo and triphenylmethane dyes by Pseudomonas pseudomallei 13NA. Journal of the Society of Dyers and Colourists 97, 166–169.Google Scholar
  22. Zimmermann, T., Kulla, H.G. & Leisinger, T. 1982 Properties of purified orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF 46. European Journal of Biochemistry 129, 197–203.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • C. Valli Nachiyar
    • 1
  • G. Suseela Rajkumar
    • 1
  1. 1.Bacteriology LaboratoryCentral Leather Research InstituteChennaiIndia

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