Journal of Applied Phycology

, Volume 15, Issue 4, pp 345–349 | Cite as

Transient expression of lacZ in particle bombarded Gracilaria changii (Gracilariales, Rhodophyta)

  • Sook-Yee Gan
  • Song Qin
  • Rofina Yasmin Othman
  • Daozhan Yu
  • Siew-Moi Phang
Note

Abstract

Using a Biolistic PDS 1000/He system, healthy thalli of Gracilaria changii were bombarded with gold particles coated with plasmid DNA containing the lacZ reporter gene. Transient expression of lacZ was observed in bombarded thalli under the rupture-disc pressures of 4482, 6206, 7584 and 8963 KPa, two days after bombardment. Although G. changii exhibits a slight blue background, positive expression and the background colour can be clearly differentiated. The results indicate that lacZ could be a useful reporter gene and that SV40 promoter could be an effective promoter for Gracilaria transformation.

Gracilaria lacZ Malaysia Particle bombardment Seaweed SV40 promoter Transient expression 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Sook-Yee Gan
    • 1
  • Song Qin
    • 2
  • Rofina Yasmin Othman
    • 3
  • Daozhan Yu
    • 2
  • Siew-Moi Phang
    • 3
  1. 1.Institute of Postgraduate StudiesUniversity of MalayaKuala LumpurMalaysia
  2. 2.Institute of OceanologyChinese Academy of ScienceQingdaoChina
  3. 3.Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia

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