Microbial decolorization of azo dyes and dye industry effluent by Fomes lividus

  • K. Selvam
  • K. Swaminathan
  • Keon-Sang Chae


The white rot fungus, Fomes lividus, was isolated from the logs of Shorea robusta in the Western Ghats region of Tamil Nadu, India. The fungus was tested for decolorization of azo dyes such as orange G (50 μM) congo red (50 μM) amido black 10B (25 μM) and also for colour removal from dye industry effluents. The results revealed that the fungus could remove only 30.8% of orange G in the synthetic solution, whereas congo red and amido black 10B were removed by 74.0 and 98.9% respectively. A dye industry effluent was treated by the fungus in batch and continuous mode. In batch mode treatment, a maximum decolorization of 84.4% was achieved on day 4, and in continuous mode a maximum decolorization of 37.5% was obtained on day 5. The colour removal by the basidiomycete fungus might be due to adsorption of the dyes to the mycelial surface and metabolic breakdown. These results suggested that the batch mode treatment of Fomes lividus is one of the most efficient ways for colour removal in dye industry effluents.

Azo dyes decolorization dye industry effluent Fomes lividus treatment 


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  1. Abadulla, E., Tzanov, T., Costa, S., Robra, K.H., Paulo, A.C. & Gubitz, G.M. 2000 Decolorization and detoxification of textile dyes with a laccase from Trametes hirsute. Applied and Environmental Microbiology 66, 3357–3362.PubMedGoogle Scholar
  2. Bakshi, B.K. 1971 Indian Polyporaceae-on Trees and Timbers. New Delhi: Indian Council for Agricultural Research (ICAR) publication. pp. 80–81.Google Scholar
  3. Cripps, C., Bumpus, J.A. & Aust, S.D. 1990 Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysosporium. Applied and Environmental Microbiology 56, 1114–1118.PubMedGoogle Scholar
  4. Gilbertson, R.L. & Ryvarden, L. 1986 North American Polypores, Olso: Fungiflora, vol. 1, p. 433.Google Scholar
  5. Glenn, J.K. & Gold, M.H. 1983 Decolorization of several polymeric dyes by the lignin degrading basidiomycete Phanerochaete chrysosporium. Applied and Environmental Microbiology 45, 1741–1747.Google Scholar
  6. Heinfling, A., Bergbaur, M. & Szewzyk, U. 1997 Biodegradation of azo and phthalocyanine dyes by Trametes versicolor and Bjerkandera adusta. Applied Microbiology and Biotechnology 48, 261–266.Google Scholar
  7. Janshekar, H. & Fiechter, A. 1988 Cultivation of Phanerochaete chrysosporium and production of lignin peroxidase in submerged stirred tank reactors. Journal of Biotechnology 8, 97–112.Google Scholar
  8. Kling, S.H. & Neto, J.S.A. 1991 Oxidation of methylene blue by crude lignin peroxidase from Phanerochaete chrysosporium. Journal of Biotechnology 21, 295–300.Google Scholar
  9. Knapp, J.S., Newby, P.S. & Reece, L.P. 1995 Decolorization of dye by wood rotting basidiomycete fungi. Enzyme and Microbial Technology 17, 664–668.Google Scholar
  10. Matthew, T. & Bumpus, J.A. 1998 Biodegradation of congo red by Phanerochaete chrysosporium. Water Research 32, 1713–1717.Google Scholar
  11. Ollikka, P., Alhonmaki, K., Leppanen, V.M., Gluumoff, T., Raijola, T. & Suominent, I. 1993 Decolorization of azo triphenylmethane, heterocyclic and polymeric dyes by the lignin peroxidase isozymes from Phanerochaete chrysosporium. Applied and Environmental Microbiology 59, 4010–4016.Google Scholar
  12. Paszezynski, A., Pasti, A., Goszezynski, M.S., Crawford, D.L. & Crawford, R.I. 1991 New approach to improve degradation of recalcitrant azo dyes by Streptomyces spp. and Phanerochaete chrysosporium. Enzyme and Microbial Technology 13, 378–384.Google Scholar
  13. Pierce, J. 1994 Colour in textile effluents-the origins of the problem. Journal of the Society of Dyers and Colourists 110, 131–134.Google Scholar
  14. Rafii, F., Franklin, W. & Cermiglia, C.E. 1990 Azoreductase activity of anaerobic bacteria isolated from human intestinal microflora. Applied and Environmental Microbiology 56, 2146–2151.PubMedGoogle Scholar
  15. Rodriguez, E., Pickard, M.A. & Duhalt, R.V. 1999 Industrial decolorization by laccase from ligninolytic fungi. Current Microbiology 38, 27–32.PubMedGoogle Scholar
  16. Spadaro, J.T., Gold, M.H. & Renganathan, V. 1992 Degradation of azo dyes by the lignin degrading fungus Phanerochaete chrysosporium. Applied and Environmental Microbiology 58, 2397–2401.PubMedGoogle Scholar
  17. Spadaro, J.T., Lorne, I. & Renganathan, V. 1994 Hydroxyl radical mediated degradation or azo dyes: evidence for benzene generation. Environmental Science and Technology 28, 1389–1393.Google Scholar
  18. Vyas, B.R.M. & Molitoris, H.P. 1995 Involvement of an extracellular H2O2-dependent ligninolytic activity of the white rot fungus Pleurotus ostrus in the decolourisation of remazol brilliant blue R. Applied and Environmental Microbiology 61, 3919–3927.PubMedGoogle Scholar
  19. Wang, Y. & Yu, J. 1998 Adsorption and degradation of synthetic dyes on the mycelium of Trametes versicolor. Water Science and Technology 38, 233–238.Google Scholar
  20. Watling, R. 1971 Basiodiomycetes: homobasidiomycetidae. In Methods in Microbiology, ed. Booth, C. pp. 219–236. London and New York: Academic press. ISBN 0–12–521504–5.Google Scholar
  21. Young, L. & Yu, J. 1997 Ligninase catalysed decolorization of synthetic dyes. Water Research 31, 1187–1193.Google Scholar
  22. Zollinger, H. 1987 Color Chemistry Synthesis, Properties and Applications of Organic Dyes and Pigments. New York: VCH Publishers. pp. 92–102. ISBN 0–89573421–4.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • K. Selvam
    • 1
  • K. Swaminathan
    • 1
  • Keon-Sang Chae
    • 2
  1. 1.Department of BiotechnologyBharathiar UniversityCoimbatoreIndia
  2. 2.Division of Biological Sciencesand Basic Science Research Institute, Chonbuk National UniversityChonju, ChonbukRepublic of Korea

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