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Novel maltotriose esters enhance biodegradation of Aroclor 1242 by Burkholderia cepacia LB400

  • M. Ferrer
  • P. Golyshin
  • K.N. Timmis
Article

Abstract

The objective of this research was to evaluate the effect of enzymatically synthesized maltotriose fatty acid monoesters (Ferrer, M., et al. 2000 Tetrahedron56, 4053–4061) on Aroclor 1242 solubilization and biodegradation. Three forms of the surfactant, laurate, palmitate and stearate monoester, were tested. Potential enhancement of solubilization of hydrophobic substances mediated by these non-ionic surfactants was exploited in this study. A polychlorinated biphenyl (PCB) degrading organism, Burkholderia cepacia LB400, was also selected. It was found that all surfactants were effective in solubilizing Aroclor 1242 but the rate of Aroclor 1242 biodegradation proceeded rapidly only in the presence of 6′′-O-palmitoylmaltotriose. For example, the addition of 48 mg 6′′-O-palmitoylmaltotriose/l increased the apparent solubility from 140 to 305 μg/l. As a result, only 8% of the Aroclor remained at the end of 24 h incubation. In contrast, 49.2% of the Aroclor 1242 remained in the absence of surfactant. It appears that maltotriose fatty acid monoesters can significantly increase the bioavailability, and thereby accelerate the biodegradation of highly chlorinated PCBs, particularly Aroclor 1242, by Burkholderia cepacia LB400. The possibility of obtaining these biodegradable surfactants with high yield, easy recovery and high purity by using a new enzymatic methodology, makes maltotriose esters available for bioremediation purposes.

Aroclor 1242 bioremediation Burkholderia cepacia LB400 lipase sugar esters 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • M. Ferrer
    • 1
  • P. Golyshin
    • 1
  • K.N. Timmis
    • 1
  1. 1.Department of MicrobiologyGBF – German Research Center for Biotechnology, Mascheroder Weg 1, D-BraunschweigGermany; Tel.:

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